Probing the Intracellular Bio-Nano Interface in Different Cell Lines with Gold Nanostars
Gold nanostars are a versatile plasmonic nanomaterial with many applications in bioanalysis. Their interactions with animal cells of three different cell lines are studied here at the molecular and ultrastructural level at an early stage of endolysosomal processing. Using the gold nanostars themselv...
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doaj-6d273113335b411ea3999bf224fc6ea62021-04-30T23:00:52ZengMDPI AGNanomaterials2079-49912021-04-01111183118310.3390/nano11051183Probing the Intracellular Bio-Nano Interface in Different Cell Lines with Gold NanostarsCecilia Spedalieri0Gergo Péter Szekeres1Stephan Werner2Peter Guttmann3Janina Kneipp4Department of Chemistry, Humboldt-Universität zu Berlin, Brook-Taylor-Str. 2, 12489 Berlin, GermanyDepartment of Chemistry, Humboldt-Universität zu Berlin, Brook-Taylor-Str. 2, 12489 Berlin, GermanyDepartment X-ray Microscopy, Helmholtz-Zentrum Berlin für Materialien und Energie GmbH, Albert-Einstein-Str. 15, 12489 Berlin, GermanyDepartment X-ray Microscopy, Helmholtz-Zentrum Berlin für Materialien und Energie GmbH, Albert-Einstein-Str. 15, 12489 Berlin, GermanyDepartment of Chemistry, Humboldt-Universität zu Berlin, Brook-Taylor-Str. 2, 12489 Berlin, GermanyGold nanostars are a versatile plasmonic nanomaterial with many applications in bioanalysis. Their interactions with animal cells of three different cell lines are studied here at the molecular and ultrastructural level at an early stage of endolysosomal processing. Using the gold nanostars themselves as substrate for surface-enhanced Raman scattering, their protein corona and the molecules in the endolysosomal environment were characterized. Localization, morphology, and size of the nanostar aggregates in the endolysosomal compartment of the cells were probed by cryo soft-X-ray nanotomography. The processing of the nanostars by macrophages of cell line J774 differed greatly from that in the fibroblast cell line 3T3 and in the epithelial cell line HCT-116, and the structure and composition of the biomolecular corona was found to resemble that of spherical gold nanoparticles in the same cells. Data obtained with gold nanostars of varied morphology indicate that the biomolecular interactions at the surface in vivo are influenced by the spike length, with increased interaction with hydrophobic groups of proteins and lipids for longer spike lengths, and independent of the cell line. The results will support optimized nanostar synthesis and delivery for sensing, imaging, and theranostics.https://www.mdpi.com/2079-4991/11/5/1183gold nanostarsSERSnanoparticle uptakenanotomographyendocytosisprotein corona |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Cecilia Spedalieri Gergo Péter Szekeres Stephan Werner Peter Guttmann Janina Kneipp |
spellingShingle |
Cecilia Spedalieri Gergo Péter Szekeres Stephan Werner Peter Guttmann Janina Kneipp Probing the Intracellular Bio-Nano Interface in Different Cell Lines with Gold Nanostars Nanomaterials gold nanostars SERS nanoparticle uptake nanotomography endocytosis protein corona |
author_facet |
Cecilia Spedalieri Gergo Péter Szekeres Stephan Werner Peter Guttmann Janina Kneipp |
author_sort |
Cecilia Spedalieri |
title |
Probing the Intracellular Bio-Nano Interface in Different Cell Lines with Gold Nanostars |
title_short |
Probing the Intracellular Bio-Nano Interface in Different Cell Lines with Gold Nanostars |
title_full |
Probing the Intracellular Bio-Nano Interface in Different Cell Lines with Gold Nanostars |
title_fullStr |
Probing the Intracellular Bio-Nano Interface in Different Cell Lines with Gold Nanostars |
title_full_unstemmed |
Probing the Intracellular Bio-Nano Interface in Different Cell Lines with Gold Nanostars |
title_sort |
probing the intracellular bio-nano interface in different cell lines with gold nanostars |
publisher |
MDPI AG |
series |
Nanomaterials |
issn |
2079-4991 |
publishDate |
2021-04-01 |
description |
Gold nanostars are a versatile plasmonic nanomaterial with many applications in bioanalysis. Their interactions with animal cells of three different cell lines are studied here at the molecular and ultrastructural level at an early stage of endolysosomal processing. Using the gold nanostars themselves as substrate for surface-enhanced Raman scattering, their protein corona and the molecules in the endolysosomal environment were characterized. Localization, morphology, and size of the nanostar aggregates in the endolysosomal compartment of the cells were probed by cryo soft-X-ray nanotomography. The processing of the nanostars by macrophages of cell line J774 differed greatly from that in the fibroblast cell line 3T3 and in the epithelial cell line HCT-116, and the structure and composition of the biomolecular corona was found to resemble that of spherical gold nanoparticles in the same cells. Data obtained with gold nanostars of varied morphology indicate that the biomolecular interactions at the surface in vivo are influenced by the spike length, with increased interaction with hydrophobic groups of proteins and lipids for longer spike lengths, and independent of the cell line. The results will support optimized nanostar synthesis and delivery for sensing, imaging, and theranostics. |
topic |
gold nanostars SERS nanoparticle uptake nanotomography endocytosis protein corona |
url |
https://www.mdpi.com/2079-4991/11/5/1183 |
work_keys_str_mv |
AT ceciliaspedalieri probingtheintracellularbionanointerfaceindifferentcelllineswithgoldnanostars AT gergopeterszekeres probingtheintracellularbionanointerfaceindifferentcelllineswithgoldnanostars AT stephanwerner probingtheintracellularbionanointerfaceindifferentcelllineswithgoldnanostars AT peterguttmann probingtheintracellularbionanointerfaceindifferentcelllineswithgoldnanostars AT janinakneipp probingtheintracellularbionanointerfaceindifferentcelllineswithgoldnanostars |
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1721497397009317888 |