Bacterial etiology of sputum from tuberculosis suspected patients and antibiogram of the isolates
Abstract Objective The current study aims to explore the bacteriology of sputum of tuberculosis (TB) suspected patients. A cross-sectional study was carried out in the sputum samples of 150 TB suspected patients visiting District Public Health Office, Bharatpur, Nepal. The samples were subjected to...
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doaj-6d2951580c144ba38ff2385fd11921332020-11-25T04:02:18ZengBMCBMC Research Notes1756-05002020-11-011311610.1186/s13104-020-05369-8Bacterial etiology of sputum from tuberculosis suspected patients and antibiogram of the isolatesRamesh Sharma Regmi0Sujan Khadka1Sanjeep Sapkota2Sanjib Adhikari3Khuma Kumari Dhakal4Bishnu Dhakal5Bandana Lamsal6Sarad Chandra Kafle7Department of Microbiology, Birendra Multiple Campus, Tribhuvan UniversityDepartment of Microbiology, Birendra Multiple Campus, Tribhuvan UniversityDepartment of Microbiology, Birendra Multiple Campus, Tribhuvan UniversityDepartment of Microbiology, Birendra Multiple Campus, Tribhuvan UniversityDepartment of Microbiology, Birendra Multiple Campus, Tribhuvan UniversityDepartment of Microbiology, Birendra Multiple Campus, Tribhuvan UniversityDepartment of Microbiology, Birendra Multiple Campus, Tribhuvan UniversityDepartment of Microbiology, Birendra Multiple Campus, Tribhuvan UniversityAbstract Objective The current study aims to explore the bacteriology of sputum of tuberculosis (TB) suspected patients. A cross-sectional study was carried out in the sputum samples of 150 TB suspected patients visiting District Public Health Office, Bharatpur, Nepal. The samples were subjected to cultural, microscopic and biochemical analyses for the identification of the isolates. In addition, antibiotic susceptibility tests were carried out with a special focus on ESBL and MBL production following Clinical and Laboratory Standard Institute guidelines. Results Bacterial growth was recovered in 47% (71/150) of the TB suspected patients of which 12.66% (19/150) had pulmonary TB infection. Streptococcus spp. (9%) and Pseudomonas aeruginosa (9%) were the most frequently isolated bacteria. Enterobacteriaceae accounted for 35% of the total isolates. Occurrence of bacterial pathogens was more in males (69%) than in females (31%).The incidence of bacterial pathogen was seen associated with gender of the patients and with the TB infection (p < 0.05) but independent with age of the patients and HIV infection (p > 0.05). Tetracycline was effective against Streptococcus spp. whereas gentamicin was effective against Bacillus species. Imipenem and co-trimoxazole were effective drugs for Gram-negative isolates. Among 83 isolates, 35 were multi-drug resistant, 9 were ESBL producers and 4 were MBL producers.http://link.springer.com/article/10.1186/s13104-020-05369-8TuberculosisRespiratory tract infectionMDRSputum |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ramesh Sharma Regmi Sujan Khadka Sanjeep Sapkota Sanjib Adhikari Khuma Kumari Dhakal Bishnu Dhakal Bandana Lamsal Sarad Chandra Kafle |
spellingShingle |
Ramesh Sharma Regmi Sujan Khadka Sanjeep Sapkota Sanjib Adhikari Khuma Kumari Dhakal Bishnu Dhakal Bandana Lamsal Sarad Chandra Kafle Bacterial etiology of sputum from tuberculosis suspected patients and antibiogram of the isolates BMC Research Notes Tuberculosis Respiratory tract infection MDR Sputum |
author_facet |
Ramesh Sharma Regmi Sujan Khadka Sanjeep Sapkota Sanjib Adhikari Khuma Kumari Dhakal Bishnu Dhakal Bandana Lamsal Sarad Chandra Kafle |
author_sort |
Ramesh Sharma Regmi |
title |
Bacterial etiology of sputum from tuberculosis suspected patients and antibiogram of the isolates |
title_short |
Bacterial etiology of sputum from tuberculosis suspected patients and antibiogram of the isolates |
title_full |
Bacterial etiology of sputum from tuberculosis suspected patients and antibiogram of the isolates |
title_fullStr |
Bacterial etiology of sputum from tuberculosis suspected patients and antibiogram of the isolates |
title_full_unstemmed |
Bacterial etiology of sputum from tuberculosis suspected patients and antibiogram of the isolates |
title_sort |
bacterial etiology of sputum from tuberculosis suspected patients and antibiogram of the isolates |
publisher |
BMC |
series |
BMC Research Notes |
issn |
1756-0500 |
publishDate |
2020-11-01 |
description |
Abstract Objective The current study aims to explore the bacteriology of sputum of tuberculosis (TB) suspected patients. A cross-sectional study was carried out in the sputum samples of 150 TB suspected patients visiting District Public Health Office, Bharatpur, Nepal. The samples were subjected to cultural, microscopic and biochemical analyses for the identification of the isolates. In addition, antibiotic susceptibility tests were carried out with a special focus on ESBL and MBL production following Clinical and Laboratory Standard Institute guidelines. Results Bacterial growth was recovered in 47% (71/150) of the TB suspected patients of which 12.66% (19/150) had pulmonary TB infection. Streptococcus spp. (9%) and Pseudomonas aeruginosa (9%) were the most frequently isolated bacteria. Enterobacteriaceae accounted for 35% of the total isolates. Occurrence of bacterial pathogens was more in males (69%) than in females (31%).The incidence of bacterial pathogen was seen associated with gender of the patients and with the TB infection (p < 0.05) but independent with age of the patients and HIV infection (p > 0.05). Tetracycline was effective against Streptococcus spp. whereas gentamicin was effective against Bacillus species. Imipenem and co-trimoxazole were effective drugs for Gram-negative isolates. Among 83 isolates, 35 were multi-drug resistant, 9 were ESBL producers and 4 were MBL producers. |
topic |
Tuberculosis Respiratory tract infection MDR Sputum |
url |
http://link.springer.com/article/10.1186/s13104-020-05369-8 |
work_keys_str_mv |
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