Analysis of Salmonella enterica serovar Enteritidis isolates from chickens and chicken meat products in Malaysia using PFGE, and MLST
Abstract Background Salmonella is a very important foodborne pathogen causing illness in humans. The emergence of drug-resistant strains also constitutes a serious worry to global health and livestock productivity. This study investigated Salmonella isolates from chicken and chicken meat products us...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2020-10-01
|
Series: | BMC Veterinary Research |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s12917-020-02605-y |
id |
doaj-6d49543d85c84707be2b45ee467aad04 |
---|---|
record_format |
Article |
spelling |
doaj-6d49543d85c84707be2b45ee467aad042020-11-25T02:46:40ZengBMCBMC Veterinary Research1746-61482020-10-011611810.1186/s12917-020-02605-yAnalysis of Salmonella enterica serovar Enteritidis isolates from chickens and chicken meat products in Malaysia using PFGE, and MLSTZunita Zakaria0Latiffah Hassan1Zawiyah Sharif2Norazah Ahmad3Rohaya Mohd Ali4Suraya Amir Husin5Nor Hazrin binti Abd Hazis6Nor Fitriah Mohamed Sohaimi7Shafini Abu Bakar8Bashiru Garba9Institute of Bioscience, Universiti Putra MalaysiaBacteriology Laboratory, Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra MalaysiaFood Safety and Quality Division, Ministry of HealthInfectious Diseases Research Centre, Institute for Medical Research, National Institutes of HealthDiagnostic and Quality Assurance Division, Department of Veterinary Services, Ministry of Agriculture & Agro-Based IndustryInfectious Diseases Research Centre, Institute for Medical Research, National Institutes of HealthInfectious Diseases Research Centre, Institute for Medical Research, National Institutes of HealthBacteriology Laboratory, Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra MalaysiaFood Safety and Quality Division, Ministry of HealthInstitute of Bioscience, Universiti Putra MalaysiaAbstract Background Salmonella is a very important foodborne pathogen causing illness in humans. The emergence of drug-resistant strains also constitutes a serious worry to global health and livestock productivity. This study investigated Salmonella isolates from chicken and chicken meat products using the phenotypic antimicrobial screening as well as the molecular characteristics of Salmonella isolates. Upon serotyping of the isolates, the antimicrobial susceptibility profiling using a panel of 9 commonly used antimicrobials was done. Subsequently, the molecular profiles of all the isolates were further determined using Pulsed Field Gel Electrophoresis (PFGE) and the Whole Genome Multi-Locus Sequence Type (wgMLST) analysis in order to obtain the sequence types. Results The PFGE data was input into FPQuest software, and the dendrogram generated was studied for possible genetic relatedness among the isolates. All the isolates were found to belong to the Salmonella Enteritidis serotype with notable resistance to tetracycline, gentamycin, streptomycin, and sulfadimidine. The S. Enteritidis isolates tested predominantly subtyped into the ST11 and ST1925, which was found to be a single cell variant of ST11. The STs were found to occur in chicken meats, foods, and live chicken cloacal swabs, which may indicate the persistence of the bacteria in multiple foci. Conclusion The data demonstrate the presence of S. Enteritidis among chickens, indicating its preference and reservoir status for enteric Salmonella pathogens.http://link.springer.com/article/10.1186/s12917-020-02605-ySalmonella EnteritidisChicken meat productsPulse field gel electrophoresisMulti-locus sequence typingWhole-genome sequencingAntimicrobial resistance |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zunita Zakaria Latiffah Hassan Zawiyah Sharif Norazah Ahmad Rohaya Mohd Ali Suraya Amir Husin Nor Hazrin binti Abd Hazis Nor Fitriah Mohamed Sohaimi Shafini Abu Bakar Bashiru Garba |
spellingShingle |
Zunita Zakaria Latiffah Hassan Zawiyah Sharif Norazah Ahmad Rohaya Mohd Ali Suraya Amir Husin Nor Hazrin binti Abd Hazis Nor Fitriah Mohamed Sohaimi Shafini Abu Bakar Bashiru Garba Analysis of Salmonella enterica serovar Enteritidis isolates from chickens and chicken meat products in Malaysia using PFGE, and MLST BMC Veterinary Research Salmonella Enteritidis Chicken meat products Pulse field gel electrophoresis Multi-locus sequence typing Whole-genome sequencing Antimicrobial resistance |
author_facet |
Zunita Zakaria Latiffah Hassan Zawiyah Sharif Norazah Ahmad Rohaya Mohd Ali Suraya Amir Husin Nor Hazrin binti Abd Hazis Nor Fitriah Mohamed Sohaimi Shafini Abu Bakar Bashiru Garba |
author_sort |
Zunita Zakaria |
title |
Analysis of Salmonella enterica serovar Enteritidis isolates from chickens and chicken meat products in Malaysia using PFGE, and MLST |
title_short |
Analysis of Salmonella enterica serovar Enteritidis isolates from chickens and chicken meat products in Malaysia using PFGE, and MLST |
title_full |
Analysis of Salmonella enterica serovar Enteritidis isolates from chickens and chicken meat products in Malaysia using PFGE, and MLST |
title_fullStr |
Analysis of Salmonella enterica serovar Enteritidis isolates from chickens and chicken meat products in Malaysia using PFGE, and MLST |
title_full_unstemmed |
Analysis of Salmonella enterica serovar Enteritidis isolates from chickens and chicken meat products in Malaysia using PFGE, and MLST |
title_sort |
analysis of salmonella enterica serovar enteritidis isolates from chickens and chicken meat products in malaysia using pfge, and mlst |
publisher |
BMC |
series |
BMC Veterinary Research |
issn |
1746-6148 |
publishDate |
2020-10-01 |
description |
Abstract Background Salmonella is a very important foodborne pathogen causing illness in humans. The emergence of drug-resistant strains also constitutes a serious worry to global health and livestock productivity. This study investigated Salmonella isolates from chicken and chicken meat products using the phenotypic antimicrobial screening as well as the molecular characteristics of Salmonella isolates. Upon serotyping of the isolates, the antimicrobial susceptibility profiling using a panel of 9 commonly used antimicrobials was done. Subsequently, the molecular profiles of all the isolates were further determined using Pulsed Field Gel Electrophoresis (PFGE) and the Whole Genome Multi-Locus Sequence Type (wgMLST) analysis in order to obtain the sequence types. Results The PFGE data was input into FPQuest software, and the dendrogram generated was studied for possible genetic relatedness among the isolates. All the isolates were found to belong to the Salmonella Enteritidis serotype with notable resistance to tetracycline, gentamycin, streptomycin, and sulfadimidine. The S. Enteritidis isolates tested predominantly subtyped into the ST11 and ST1925, which was found to be a single cell variant of ST11. The STs were found to occur in chicken meats, foods, and live chicken cloacal swabs, which may indicate the persistence of the bacteria in multiple foci. Conclusion The data demonstrate the presence of S. Enteritidis among chickens, indicating its preference and reservoir status for enteric Salmonella pathogens. |
topic |
Salmonella Enteritidis Chicken meat products Pulse field gel electrophoresis Multi-locus sequence typing Whole-genome sequencing Antimicrobial resistance |
url |
http://link.springer.com/article/10.1186/s12917-020-02605-y |
work_keys_str_mv |
AT zunitazakaria analysisofsalmonellaentericaserovarenteritidisisolatesfromchickensandchickenmeatproductsinmalaysiausingpfgeandmlst AT latiffahhassan analysisofsalmonellaentericaserovarenteritidisisolatesfromchickensandchickenmeatproductsinmalaysiausingpfgeandmlst AT zawiyahsharif analysisofsalmonellaentericaserovarenteritidisisolatesfromchickensandchickenmeatproductsinmalaysiausingpfgeandmlst AT norazahahmad analysisofsalmonellaentericaserovarenteritidisisolatesfromchickensandchickenmeatproductsinmalaysiausingpfgeandmlst AT rohayamohdali analysisofsalmonellaentericaserovarenteritidisisolatesfromchickensandchickenmeatproductsinmalaysiausingpfgeandmlst AT surayaamirhusin analysisofsalmonellaentericaserovarenteritidisisolatesfromchickensandchickenmeatproductsinmalaysiausingpfgeandmlst AT norhazrinbintiabdhazis analysisofsalmonellaentericaserovarenteritidisisolatesfromchickensandchickenmeatproductsinmalaysiausingpfgeandmlst AT norfitriahmohamedsohaimi analysisofsalmonellaentericaserovarenteritidisisolatesfromchickensandchickenmeatproductsinmalaysiausingpfgeandmlst AT shafiniabubakar analysisofsalmonellaentericaserovarenteritidisisolatesfromchickensandchickenmeatproductsinmalaysiausingpfgeandmlst AT bashirugarba analysisofsalmonellaentericaserovarenteritidisisolatesfromchickensandchickenmeatproductsinmalaysiausingpfgeandmlst |
_version_ |
1724756696033656832 |