Pseudo-outbreak of stenotrophomonas maltophilia isolated from kidney stones and attributed to inadequate sterilization: Investigation of molecular typing and clonal relationship
Stenotrophomonas maltophilia is a gram-negative bacterium. Hospitals can be a source of S. maltophilia because it adheres to nonliving surfaces and forms a biofilm. This study was performed to investigate the clonal relationship between S. maltophilia isolates obtained from kidney stone samples. Sam...
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doaj-6d4e9cf5fcd44f69aa8cfeb98f3669d62021-03-24T11:50:38ZengSociety of TURAZ AKADEMI Medicine Science2147-06342020-09-01935899410.5455/medscience.2020.09.922380085Pseudo-outbreak of stenotrophomonas maltophilia isolated from kidney stones and attributed to inadequate sterilization: Investigation of molecular typing and clonal relationshipReyhan Yis0Selma TosunEmine Deniz BayramOzlem Gamze GulfidanTansu DegirmenciBulent GunlusoyBaris OtluCigdem KuzucuIzmir Bozyaka Training and Research Hospital, Department of Medical Microbiology, Izmir, Turkey Izmir Bozyaka Training and Research Hospital, Department of Infectious Diseases and Clinical Microbiology, Izmir, Turkey Izmir Bozyaka Training and Research Hospital, Department of Urology, Izmir, Turkey Inonu University Faculty of Medicine, Department of Medical Microbiology, Malatya, TurkeyStenotrophomonas maltophilia is a gram-negative bacterium. Hospitals can be a source of S. maltophilia because it adheres to nonliving surfaces and forms a biofilm. This study was performed to investigate the clonal relationship between S. maltophilia isolates obtained from kidney stone samples. Samples of kidney stones taken from patients and surrogate samples from nephroscopes, cleaning solution, disinfectant solution were included in the study. The clonal relationship between isolates was determined by PFGE. S. maltophilia was isolated from 34 of 94 kidney stone samples sent from the urology operating room between July 2017 and January 2018. A total of 26 S. maltophilia strains (21 from kidney stone samples, three from nephroscopes, and two from urine culture) were isolated. PFGE showed that the 21 kidney stone isolates and the 3 S. maltophilia isolates obtained from the nephroscope belonged to the same clone. The two urine culture isolates showed no clonal relationship to the outbreak isolates and were considered sporadic. Molecular typing confirmed that this pseudo-outbreak was attributed to inadequate disinfection of the nephroscopes. After disinfection protocols were reviewed and revised as needed, especially regarding the removal of organic material from nephroscopes after use, no further bacterial growth was detected from kidney stone specimens obtained with nephroscopes. [Med-Science 2020; 9(3.000): 589-94]http://www.ejmanager.com/fulltextpdf.php?mno=80085stenotrophomonas maltophiliapseudo-outbreakinadequate sterilizationmolecular typingclonal relationship |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Reyhan Yis Selma Tosun Emine Deniz Bayram Ozlem Gamze Gulfidan Tansu Degirmenci Bulent Gunlusoy Baris Otlu Cigdem Kuzucu |
spellingShingle |
Reyhan Yis Selma Tosun Emine Deniz Bayram Ozlem Gamze Gulfidan Tansu Degirmenci Bulent Gunlusoy Baris Otlu Cigdem Kuzucu Pseudo-outbreak of stenotrophomonas maltophilia isolated from kidney stones and attributed to inadequate sterilization: Investigation of molecular typing and clonal relationship Medicine Science stenotrophomonas maltophilia pseudo-outbreak inadequate sterilization molecular typing clonal relationship |
author_facet |
Reyhan Yis Selma Tosun Emine Deniz Bayram Ozlem Gamze Gulfidan Tansu Degirmenci Bulent Gunlusoy Baris Otlu Cigdem Kuzucu |
author_sort |
Reyhan Yis |
title |
Pseudo-outbreak of stenotrophomonas maltophilia isolated from kidney stones and attributed to inadequate sterilization: Investigation of molecular typing and clonal relationship |
title_short |
Pseudo-outbreak of stenotrophomonas maltophilia isolated from kidney stones and attributed to inadequate sterilization: Investigation of molecular typing and clonal relationship |
title_full |
Pseudo-outbreak of stenotrophomonas maltophilia isolated from kidney stones and attributed to inadequate sterilization: Investigation of molecular typing and clonal relationship |
title_fullStr |
Pseudo-outbreak of stenotrophomonas maltophilia isolated from kidney stones and attributed to inadequate sterilization: Investigation of molecular typing and clonal relationship |
title_full_unstemmed |
Pseudo-outbreak of stenotrophomonas maltophilia isolated from kidney stones and attributed to inadequate sterilization: Investigation of molecular typing and clonal relationship |
title_sort |
pseudo-outbreak of stenotrophomonas maltophilia isolated from kidney stones and attributed to inadequate sterilization: investigation of molecular typing and clonal relationship |
publisher |
Society of TURAZ AKADEMI |
series |
Medicine Science |
issn |
2147-0634 |
publishDate |
2020-09-01 |
description |
Stenotrophomonas maltophilia is a gram-negative bacterium. Hospitals can be a source of S. maltophilia because it adheres to nonliving surfaces and forms a biofilm. This study was performed to investigate the clonal relationship between S. maltophilia isolates obtained from kidney stone samples. Samples of kidney stones taken from patients and surrogate samples from nephroscopes, cleaning solution, disinfectant solution were included in the study. The clonal relationship between isolates was determined by PFGE. S. maltophilia was isolated from 34 of 94 kidney stone samples sent from the urology operating room between July 2017 and January 2018. A total of 26 S. maltophilia strains (21 from kidney stone samples, three from nephroscopes, and two from urine culture) were isolated. PFGE showed that the 21 kidney stone isolates and the 3 S. maltophilia isolates obtained from the nephroscope belonged to the same clone. The two urine culture isolates showed no clonal relationship to the outbreak isolates and were considered sporadic. Molecular typing confirmed that this pseudo-outbreak was attributed to inadequate disinfection of the nephroscopes. After disinfection protocols were reviewed and revised as needed, especially regarding the removal of organic material from nephroscopes after use, no further bacterial growth was detected from kidney stone specimens obtained with nephroscopes. [Med-Science 2020; 9(3.000): 589-94] |
topic |
stenotrophomonas maltophilia pseudo-outbreak inadequate sterilization molecular typing clonal relationship |
url |
http://www.ejmanager.com/fulltextpdf.php?mno=80085 |
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