Anti-tumoral Effects of Recombinant Lactococcus lactis Strain Secreting IL-17A Cytokine
Interleukin-17A (IL-17A) is a pro-inflammatory cytokine produced by TH17 cells that participates and contributes in host defense and autoimmune disease. We have recently reported antitumor properties of the probiotic strain of Lactobacillus casei BL23 in mice and TH17 cells was shown to play an impo...
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doaj-6d4eaedc332247348d97c2f4ab193cbd2020-11-25T00:26:09ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-01-01910.3389/fmicb.2018.03355400430Anti-tumoral Effects of Recombinant Lactococcus lactis Strain Secreting IL-17A CytokineElsa Jacouton0Edgar Torres Maravilla1Anne-Sophie Boucard2Nicolas Pouderous3Ana Paula Pessoa Vilela4Ana Paula Pessoa Vilela5Isabelle Naas6Florian Chain7Vasco Azevedo8Philippe Langella9Luis G. Bermúdez-Humarán10Micalis Institute, AgroParisTech, INRA, Université Paris-Saclay, Jouy-en-Josas, FranceMicalis Institute, AgroParisTech, INRA, Université Paris-Saclay, Jouy-en-Josas, FranceMicalis Institute, AgroParisTech, INRA, Université Paris-Saclay, Jouy-en-Josas, FranceMicalis Institute, AgroParisTech, INRA, Université Paris-Saclay, Jouy-en-Josas, FranceMicalis Institute, AgroParisTech, INRA, Université Paris-Saclay, Jouy-en-Josas, FranceInstituto de Ciências Biológicas, Federal University of Minas Gerais, Belo Horizonte, BrazilMicalis Institute, AgroParisTech, INRA, Université Paris-Saclay, Jouy-en-Josas, FranceMicalis Institute, AgroParisTech, INRA, Université Paris-Saclay, Jouy-en-Josas, FranceInstituto de Ciências Biológicas, Federal University of Minas Gerais, Belo Horizonte, BrazilMicalis Institute, AgroParisTech, INRA, Université Paris-Saclay, Jouy-en-Josas, FranceMicalis Institute, AgroParisTech, INRA, Université Paris-Saclay, Jouy-en-Josas, FranceInterleukin-17A (IL-17A) is a pro-inflammatory cytokine produced by TH17 cells that participates and contributes in host defense and autoimmune disease. We have recently reported antitumor properties of the probiotic strain of Lactobacillus casei BL23 in mice and TH17 cells was shown to play an important role in this beneficial effect. In order to better understand the role of IL-17A in cancer, we constructed a recombinant strain of Lactococcus lactis producing this cytokine and we determined its biological activity in: (i) a bioassay test for the induction of IL-6 production by murine fibroblasts 3T3 L1 cells line and (ii) in a mouse allograft model of human papilloma virus (HPV)-induced cancer. Our data show that recombinant L. lactis produces and efficiently secretes biologically active IL-17A cytokine. Interestingly, ∼26% of mice intranasally treated with L. lactis-IL-17A and challenged with TC-1 cells remained tumor free over the experiment, in contrast to control mice treated with the wild type strain of L. lactis which developed 100% of aggressive tumors. In addition, the median size of the ∼74% tumor-bearing mice treated with recombinant L. lactis-IL-17A, was significantly lower than mice treated with L. lactis-wt. Altogether, our results demonstrate that intranasal administration with L. lactis secreting IL-17A results in a partial protection against TC-1-induced tumors in mice, confirming antitumor effects of this cytokine in our cancer model.https://www.frontiersin.org/article/10.3389/fmicb.2018.03355/fullLactococcus lactislactic acid bacteriaIL-17AcancerHPV |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Elsa Jacouton Edgar Torres Maravilla Anne-Sophie Boucard Nicolas Pouderous Ana Paula Pessoa Vilela Ana Paula Pessoa Vilela Isabelle Naas Florian Chain Vasco Azevedo Philippe Langella Luis G. Bermúdez-Humarán |
spellingShingle |
Elsa Jacouton Edgar Torres Maravilla Anne-Sophie Boucard Nicolas Pouderous Ana Paula Pessoa Vilela Ana Paula Pessoa Vilela Isabelle Naas Florian Chain Vasco Azevedo Philippe Langella Luis G. Bermúdez-Humarán Anti-tumoral Effects of Recombinant Lactococcus lactis Strain Secreting IL-17A Cytokine Frontiers in Microbiology Lactococcus lactis lactic acid bacteria IL-17A cancer HPV |
author_facet |
Elsa Jacouton Edgar Torres Maravilla Anne-Sophie Boucard Nicolas Pouderous Ana Paula Pessoa Vilela Ana Paula Pessoa Vilela Isabelle Naas Florian Chain Vasco Azevedo Philippe Langella Luis G. Bermúdez-Humarán |
author_sort |
Elsa Jacouton |
title |
Anti-tumoral Effects of Recombinant Lactococcus lactis Strain Secreting IL-17A Cytokine |
title_short |
Anti-tumoral Effects of Recombinant Lactococcus lactis Strain Secreting IL-17A Cytokine |
title_full |
Anti-tumoral Effects of Recombinant Lactococcus lactis Strain Secreting IL-17A Cytokine |
title_fullStr |
Anti-tumoral Effects of Recombinant Lactococcus lactis Strain Secreting IL-17A Cytokine |
title_full_unstemmed |
Anti-tumoral Effects of Recombinant Lactococcus lactis Strain Secreting IL-17A Cytokine |
title_sort |
anti-tumoral effects of recombinant lactococcus lactis strain secreting il-17a cytokine |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2019-01-01 |
description |
Interleukin-17A (IL-17A) is a pro-inflammatory cytokine produced by TH17 cells that participates and contributes in host defense and autoimmune disease. We have recently reported antitumor properties of the probiotic strain of Lactobacillus casei BL23 in mice and TH17 cells was shown to play an important role in this beneficial effect. In order to better understand the role of IL-17A in cancer, we constructed a recombinant strain of Lactococcus lactis producing this cytokine and we determined its biological activity in: (i) a bioassay test for the induction of IL-6 production by murine fibroblasts 3T3 L1 cells line and (ii) in a mouse allograft model of human papilloma virus (HPV)-induced cancer. Our data show that recombinant L. lactis produces and efficiently secretes biologically active IL-17A cytokine. Interestingly, ∼26% of mice intranasally treated with L. lactis-IL-17A and challenged with TC-1 cells remained tumor free over the experiment, in contrast to control mice treated with the wild type strain of L. lactis which developed 100% of aggressive tumors. In addition, the median size of the ∼74% tumor-bearing mice treated with recombinant L. lactis-IL-17A, was significantly lower than mice treated with L. lactis-wt. Altogether, our results demonstrate that intranasal administration with L. lactis secreting IL-17A results in a partial protection against TC-1-induced tumors in mice, confirming antitumor effects of this cytokine in our cancer model. |
topic |
Lactococcus lactis lactic acid bacteria IL-17A cancer HPV |
url |
https://www.frontiersin.org/article/10.3389/fmicb.2018.03355/full |
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