Acquisition of exogenous haem is essential for tick reproduction
Haem and iron homeostasis in most eukaryotic cells is based on a balanced flux between haem biosynthesis and haem oxygenase-mediated degradation. Unlike most eukaryotes, ticks possess an incomplete haem biosynthetic pathway and, together with other (non-haematophagous) mites, lack a gene encoding ha...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
eLife Sciences Publications Ltd
2016-03-01
|
Series: | eLife |
Subjects: | |
Online Access: | https://elifesciences.org/articles/12318 |
id |
doaj-6d8adb7c53ae4648b7652798c487c0cd |
---|---|
record_format |
Article |
spelling |
doaj-6d8adb7c53ae4648b7652798c487c0cd2021-05-05T00:17:54ZengeLife Sciences Publications LtdeLife2050-084X2016-03-01510.7554/eLife.12318Acquisition of exogenous haem is essential for tick reproductionJan Perner0Roman Sobotka1Radek Sima2Jitka Konvickova3Daniel Sojka4Pedro Lagerblad de Oliveira5Ondrej Hajdusek6Petr Kopacek7Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Ceske Budejovice, Czech Republic; Faculty of Science, University of South Bohemia, Ceske Budejovice, Czech RepublicInstitute of Microbiology, Czech Academy of Sciences, Trebon, Czech RepublicInstitute of Parasitology, Biology Centre of the Czech Academy of Sciences, Ceske Budejovice, Czech RepublicInstitute of Parasitology, Biology Centre of the Czech Academy of Sciences, Ceske Budejovice, Czech Republic; Faculty of Science, University of South Bohemia, Ceske Budejovice, Czech RepublicInstitute of Parasitology, Biology Centre of the Czech Academy of Sciences, Ceske Budejovice, Czech RepublicInstituto de Bioquímica Médica Leopoldo de Meis, Programa de Biologia Molecular e Biotecnologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil; Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular, Brasil, BrazilInstitute of Parasitology, Biology Centre of the Czech Academy of Sciences, Ceske Budejovice, Czech RepublicInstitute of Parasitology, Biology Centre of the Czech Academy of Sciences, Ceske Budejovice, Czech RepublicHaem and iron homeostasis in most eukaryotic cells is based on a balanced flux between haem biosynthesis and haem oxygenase-mediated degradation. Unlike most eukaryotes, ticks possess an incomplete haem biosynthetic pathway and, together with other (non-haematophagous) mites, lack a gene encoding haem oxygenase. We demonstrated, by membrane feeding, that ticks do not acquire bioavailable iron from haemoglobin-derived haem. However, ticks require dietary haemoglobin as an exogenous source of haem since, feeding with haemoglobin-depleted serum led to aborted embryogenesis. Supplementation of serum with haemoglobin fully restored egg fertility. Surprisingly, haemoglobin could be completely substituted by serum proteins for the provision of amino-acids in vitellogenesis. Acquired haem is distributed by haemolymph carrier protein(s) and sequestered by vitellins in the developing oocytes. This work extends, substantially, current knowledge of haem auxotrophy in ticks and underscores the importance of haem and iron metabolism as rational targets for anti-tick interventions.https://elifesciences.org/articles/12318haem auxotrophyhaem oxygenaseiron metabolismhaematophagy |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jan Perner Roman Sobotka Radek Sima Jitka Konvickova Daniel Sojka Pedro Lagerblad de Oliveira Ondrej Hajdusek Petr Kopacek |
spellingShingle |
Jan Perner Roman Sobotka Radek Sima Jitka Konvickova Daniel Sojka Pedro Lagerblad de Oliveira Ondrej Hajdusek Petr Kopacek Acquisition of exogenous haem is essential for tick reproduction eLife haem auxotrophy haem oxygenase iron metabolism haematophagy |
author_facet |
Jan Perner Roman Sobotka Radek Sima Jitka Konvickova Daniel Sojka Pedro Lagerblad de Oliveira Ondrej Hajdusek Petr Kopacek |
author_sort |
Jan Perner |
title |
Acquisition of exogenous haem is essential for tick reproduction |
title_short |
Acquisition of exogenous haem is essential for tick reproduction |
title_full |
Acquisition of exogenous haem is essential for tick reproduction |
title_fullStr |
Acquisition of exogenous haem is essential for tick reproduction |
title_full_unstemmed |
Acquisition of exogenous haem is essential for tick reproduction |
title_sort |
acquisition of exogenous haem is essential for tick reproduction |
publisher |
eLife Sciences Publications Ltd |
series |
eLife |
issn |
2050-084X |
publishDate |
2016-03-01 |
description |
Haem and iron homeostasis in most eukaryotic cells is based on a balanced flux between haem biosynthesis and haem oxygenase-mediated degradation. Unlike most eukaryotes, ticks possess an incomplete haem biosynthetic pathway and, together with other (non-haematophagous) mites, lack a gene encoding haem oxygenase. We demonstrated, by membrane feeding, that ticks do not acquire bioavailable iron from haemoglobin-derived haem. However, ticks require dietary haemoglobin as an exogenous source of haem since, feeding with haemoglobin-depleted serum led to aborted embryogenesis. Supplementation of serum with haemoglobin fully restored egg fertility. Surprisingly, haemoglobin could be completely substituted by serum proteins for the provision of amino-acids in vitellogenesis. Acquired haem is distributed by haemolymph carrier protein(s) and sequestered by vitellins in the developing oocytes. This work extends, substantially, current knowledge of haem auxotrophy in ticks and underscores the importance of haem and iron metabolism as rational targets for anti-tick interventions. |
topic |
haem auxotrophy haem oxygenase iron metabolism haematophagy |
url |
https://elifesciences.org/articles/12318 |
work_keys_str_mv |
AT janperner acquisitionofexogenoushaemisessentialfortickreproduction AT romansobotka acquisitionofexogenoushaemisessentialfortickreproduction AT radeksima acquisitionofexogenoushaemisessentialfortickreproduction AT jitkakonvickova acquisitionofexogenoushaemisessentialfortickreproduction AT danielsojka acquisitionofexogenoushaemisessentialfortickreproduction AT pedrolagerbladdeoliveira acquisitionofexogenoushaemisessentialfortickreproduction AT ondrejhajdusek acquisitionofexogenoushaemisessentialfortickreproduction AT petrkopacek acquisitionofexogenoushaemisessentialfortickreproduction |
_version_ |
1721476389655281664 |