A novel role for aquaporin-5 in enhancing microtubule organization and stability.
Aquaporin-5 (AQP5) is a water-specific channel located on the apical surface of airway epithelial cells. In addition to regulating transcellular water permeability, AQP5 can regulate paracellular permeability, though the mechanisms by which this occurs have not been determined. Microtubules also reg...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2012-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC3371026?pdf=render |
id |
doaj-6da7d456340049f78f7a90c7b65ade55 |
---|---|
record_format |
Article |
spelling |
doaj-6da7d456340049f78f7a90c7b65ade552020-11-25T01:04:22ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0176e3871710.1371/journal.pone.0038717A novel role for aquaporin-5 in enhancing microtubule organization and stability.Venkataramana K SidhayeEric ChauVasudha SrivastavaSrinivas SirimalleChinmayee BalabhadrapatruniNeil R AggarwalFranco R D'AlessioDouglas N RobinsonLandon S KingAquaporin-5 (AQP5) is a water-specific channel located on the apical surface of airway epithelial cells. In addition to regulating transcellular water permeability, AQP5 can regulate paracellular permeability, though the mechanisms by which this occurs have not been determined. Microtubules also regulate paracellular permeability. Here, we report that AQP5 promotes microtubule assembly and helps maintain the assembled microtubule steady state levels with slower turnover dynamics in cells. Specifically, reduced levels of AQP5 correlated with lower levels of assembled microtubules and decreased paracellular permeability. In contrast, overexpression of AQP5 increased assembly of microtubules, with evidence of increased MT stability, and promoted the formation of long straight microtubules in the apical domain of the epithelial cells. These findings indicate that AQP5-mediated regulation of microtubule dynamics modulates airway epithelial barrier properties and epithelial function.http://europepmc.org/articles/PMC3371026?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Venkataramana K Sidhaye Eric Chau Vasudha Srivastava Srinivas Sirimalle Chinmayee Balabhadrapatruni Neil R Aggarwal Franco R D'Alessio Douglas N Robinson Landon S King |
spellingShingle |
Venkataramana K Sidhaye Eric Chau Vasudha Srivastava Srinivas Sirimalle Chinmayee Balabhadrapatruni Neil R Aggarwal Franco R D'Alessio Douglas N Robinson Landon S King A novel role for aquaporin-5 in enhancing microtubule organization and stability. PLoS ONE |
author_facet |
Venkataramana K Sidhaye Eric Chau Vasudha Srivastava Srinivas Sirimalle Chinmayee Balabhadrapatruni Neil R Aggarwal Franco R D'Alessio Douglas N Robinson Landon S King |
author_sort |
Venkataramana K Sidhaye |
title |
A novel role for aquaporin-5 in enhancing microtubule organization and stability. |
title_short |
A novel role for aquaporin-5 in enhancing microtubule organization and stability. |
title_full |
A novel role for aquaporin-5 in enhancing microtubule organization and stability. |
title_fullStr |
A novel role for aquaporin-5 in enhancing microtubule organization and stability. |
title_full_unstemmed |
A novel role for aquaporin-5 in enhancing microtubule organization and stability. |
title_sort |
novel role for aquaporin-5 in enhancing microtubule organization and stability. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2012-01-01 |
description |
Aquaporin-5 (AQP5) is a water-specific channel located on the apical surface of airway epithelial cells. In addition to regulating transcellular water permeability, AQP5 can regulate paracellular permeability, though the mechanisms by which this occurs have not been determined. Microtubules also regulate paracellular permeability. Here, we report that AQP5 promotes microtubule assembly and helps maintain the assembled microtubule steady state levels with slower turnover dynamics in cells. Specifically, reduced levels of AQP5 correlated with lower levels of assembled microtubules and decreased paracellular permeability. In contrast, overexpression of AQP5 increased assembly of microtubules, with evidence of increased MT stability, and promoted the formation of long straight microtubules in the apical domain of the epithelial cells. These findings indicate that AQP5-mediated regulation of microtubule dynamics modulates airway epithelial barrier properties and epithelial function. |
url |
http://europepmc.org/articles/PMC3371026?pdf=render |
work_keys_str_mv |
AT venkataramanaksidhaye anovelroleforaquaporin5inenhancingmicrotubuleorganizationandstability AT ericchau anovelroleforaquaporin5inenhancingmicrotubuleorganizationandstability AT vasudhasrivastava anovelroleforaquaporin5inenhancingmicrotubuleorganizationandstability AT srinivassirimalle anovelroleforaquaporin5inenhancingmicrotubuleorganizationandstability AT chinmayeebalabhadrapatruni anovelroleforaquaporin5inenhancingmicrotubuleorganizationandstability AT neilraggarwal anovelroleforaquaporin5inenhancingmicrotubuleorganizationandstability AT francordalessio anovelroleforaquaporin5inenhancingmicrotubuleorganizationandstability AT douglasnrobinson anovelroleforaquaporin5inenhancingmicrotubuleorganizationandstability AT landonsking anovelroleforaquaporin5inenhancingmicrotubuleorganizationandstability AT venkataramanaksidhaye novelroleforaquaporin5inenhancingmicrotubuleorganizationandstability AT ericchau novelroleforaquaporin5inenhancingmicrotubuleorganizationandstability AT vasudhasrivastava novelroleforaquaporin5inenhancingmicrotubuleorganizationandstability AT srinivassirimalle novelroleforaquaporin5inenhancingmicrotubuleorganizationandstability AT chinmayeebalabhadrapatruni novelroleforaquaporin5inenhancingmicrotubuleorganizationandstability AT neilraggarwal novelroleforaquaporin5inenhancingmicrotubuleorganizationandstability AT francordalessio novelroleforaquaporin5inenhancingmicrotubuleorganizationandstability AT douglasnrobinson novelroleforaquaporin5inenhancingmicrotubuleorganizationandstability AT landonsking novelroleforaquaporin5inenhancingmicrotubuleorganizationandstability |
_version_ |
1725198627910975488 |