Characterization and Genome Analysis of a Nicotine and Nicotinic Acid-Degrading Strain Pseudomonas putida JQ581 Isolated from Marine
The presence of nicotine and nicotinic acid (NA) in the marine environment has caused great harm to human health and the natural environment. Therefore, there is an urgent need to use efficient and economical methods to remove such pollutants from the environment. In this study, a nicotine and NA-de...
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doaj-6da9640572bc480cb6c009bc0a1c3c7d2020-11-25T00:02:15ZengMDPI AGMarine Drugs1660-33972017-05-0115615610.3390/md15060156md15060156Characterization and Genome Analysis of a Nicotine and Nicotinic Acid-Degrading Strain Pseudomonas putida JQ581 Isolated from MarineAiwen Li0Jiguo Qiu1Dongzhi Chen2Jiexu Ye3Yuhong Wang4Lu Tong5Jiandong Jiang6Jianmeng Chen7College of Environment, Zhejiang University of Technology, Hangzhou 310014, ChinaCollege of Environment, Zhejiang University of Technology, Hangzhou 310014, ChinaCollege of Environment, Zhejiang University of Technology, Hangzhou 310014, ChinaCollege of Environment, Zhejiang University of Technology, Hangzhou 310014, ChinaNanjing Yuanheng Institute for Environmental Studies Co., Ltd., Nanjing 210049, ChinaNanjing Yuanheng Institute for Environmental Studies Co., Ltd., Nanjing 210049, ChinaCollege of Life Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Environment, Zhejiang University of Technology, Hangzhou 310014, ChinaThe presence of nicotine and nicotinic acid (NA) in the marine environment has caused great harm to human health and the natural environment. Therefore, there is an urgent need to use efficient and economical methods to remove such pollutants from the environment. In this study, a nicotine and NA-degrading bacterium—strain JQ581—was isolated from sediment from the East China Sea and identified as a member of Pseudomonas putida based on morphology, physio-biochemical characteristics, and 16S rDNA gene analysis. The relationship between growth and nicotine/NA degradation suggested that strain JQ581 was a good candidate for applications in the bioaugmentation treatment of nicotine/NA contamination. The degradation intermediates of nicotine are pseudooxynicotine (PN) and 3-succinoyl-pyridine (SP) based on UV, high performance liquid chromatography, and liquid chromatography-mass spectrometry analyses. However, 6-hydroxy-3-succinoyl-pyridine (HSP) was not detected. NA degradation intermediates were identified as 6-hydroxynicotinic acid (6HNA). The whole genome of strain JQ581 was sequenced and analyzed. Genome sequence analysis revealed that strain JQ581 contained the gene clusters for nicotine and NA degradation. This is the first report where a marine-derived Pseudomonas strain had the ability to degrade nicotine and NA simultaneously.http://www.mdpi.com/1660-3397/15/6/156nicotinenicotinic acidbiodegradationgenomePseudomonas putidamarine-derived bacterium |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Aiwen Li Jiguo Qiu Dongzhi Chen Jiexu Ye Yuhong Wang Lu Tong Jiandong Jiang Jianmeng Chen |
spellingShingle |
Aiwen Li Jiguo Qiu Dongzhi Chen Jiexu Ye Yuhong Wang Lu Tong Jiandong Jiang Jianmeng Chen Characterization and Genome Analysis of a Nicotine and Nicotinic Acid-Degrading Strain Pseudomonas putida JQ581 Isolated from Marine Marine Drugs nicotine nicotinic acid biodegradation genome Pseudomonas putida marine-derived bacterium |
author_facet |
Aiwen Li Jiguo Qiu Dongzhi Chen Jiexu Ye Yuhong Wang Lu Tong Jiandong Jiang Jianmeng Chen |
author_sort |
Aiwen Li |
title |
Characterization and Genome Analysis of a Nicotine and Nicotinic Acid-Degrading Strain Pseudomonas putida JQ581 Isolated from Marine |
title_short |
Characterization and Genome Analysis of a Nicotine and Nicotinic Acid-Degrading Strain Pseudomonas putida JQ581 Isolated from Marine |
title_full |
Characterization and Genome Analysis of a Nicotine and Nicotinic Acid-Degrading Strain Pseudomonas putida JQ581 Isolated from Marine |
title_fullStr |
Characterization and Genome Analysis of a Nicotine and Nicotinic Acid-Degrading Strain Pseudomonas putida JQ581 Isolated from Marine |
title_full_unstemmed |
Characterization and Genome Analysis of a Nicotine and Nicotinic Acid-Degrading Strain Pseudomonas putida JQ581 Isolated from Marine |
title_sort |
characterization and genome analysis of a nicotine and nicotinic acid-degrading strain pseudomonas putida jq581 isolated from marine |
publisher |
MDPI AG |
series |
Marine Drugs |
issn |
1660-3397 |
publishDate |
2017-05-01 |
description |
The presence of nicotine and nicotinic acid (NA) in the marine environment has caused great harm to human health and the natural environment. Therefore, there is an urgent need to use efficient and economical methods to remove such pollutants from the environment. In this study, a nicotine and NA-degrading bacterium—strain JQ581—was isolated from sediment from the East China Sea and identified as a member of Pseudomonas putida based on morphology, physio-biochemical characteristics, and 16S rDNA gene analysis. The relationship between growth and nicotine/NA degradation suggested that strain JQ581 was a good candidate for applications in the bioaugmentation treatment of nicotine/NA contamination. The degradation intermediates of nicotine are pseudooxynicotine (PN) and 3-succinoyl-pyridine (SP) based on UV, high performance liquid chromatography, and liquid chromatography-mass spectrometry analyses. However, 6-hydroxy-3-succinoyl-pyridine (HSP) was not detected. NA degradation intermediates were identified as 6-hydroxynicotinic acid (6HNA). The whole genome of strain JQ581 was sequenced and analyzed. Genome sequence analysis revealed that strain JQ581 contained the gene clusters for nicotine and NA degradation. This is the first report where a marine-derived Pseudomonas strain had the ability to degrade nicotine and NA simultaneously. |
topic |
nicotine nicotinic acid biodegradation genome Pseudomonas putida marine-derived bacterium |
url |
http://www.mdpi.com/1660-3397/15/6/156 |
work_keys_str_mv |
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