Diversity of dehalorespiring bacteria and selective enrichment of aryl halides-dechlorinating consortium from sedimentary environment near an oil refinery
This study demonstrated the potential for reductive dechlorination of aryl halides in a sedimentary environment by naturally occurring bacteria. A laboratory sediment microcosm and highly enriched, stable dechlorinating cultures were established using hydrogen as electron donor and 2,3-dichloropheno...
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doaj-6dac2f85f6af4ad7b66892a508eaeec12020-11-25T00:37:39ZengTaylor & Francis GroupJournal of Taibah University for Science1658-36552018-11-0112671172210.1080/16583655.2018.14958691495869Diversity of dehalorespiring bacteria and selective enrichment of aryl halides-dechlorinating consortium from sedimentary environment near an oil refineryWael S. El-Sayed0Saleh R. Al-Senani1Yasser Elbahloul2Taibah UniversityIslamic University of MedinaTaibah UniversityThis study demonstrated the potential for reductive dechlorination of aryl halides in a sedimentary environment by naturally occurring bacteria. A laboratory sediment microcosm and highly enriched, stable dechlorinating cultures were established using hydrogen as electron donor and 2,3-dichlorophenol (2,3DCP), monochlorophenol (MCP) and dichlorobenzene (DCB) as electron acceptors. 16S rRNA gene sequencing of dominant DGGE bands assigned detected phylotypes in chlorophenols (CPs) amended sediment microcosms to the genera Anaerospora, Pseudomonas, Desulfitobacterium, Clostridium, Mycobacterium, and Peptoclostridium, beside uncultured marine Bacterioplankton, Halochromatium and Sedimentibacter. Chlorobenzene (CB) amended sediments showed the same community in addition to Bacillus sp. Major operational taxonomic units (OTUs) in CPs enrichment cultures were assigned to Anaerospora hongkongensis, Pseudomonas stutzeri, Pseudomonas pseudoalcaligenes, Clostridium sp., Desulfitobacterium dichloroeliminans, beside unidentified marine bacterioplankton. Enrichment on DCB resulted in predominance of the same populations in addition to Peptoclostridium sp. and Dehalococcoides mccartyi. Dechlorination in enrichment cultures was mainly assigned to Desulfitobacterium and Dehalococcoides, which are both known for their ability to couple dechlorination to growth in a halorespiration way. Enrichment cultures containing Desulfitobacterium showed ortho dechlorination activity of 2,3DCP to 2 chlorophenol (2CP) and 3 chlorophenol (3CP). These results provided compelling evidence that sedimentary environments in the vicinity of oil refineries do harbor dehalorespiring bacteria capable of reductive dechlorination of various aryl halides pollutants.http://dx.doi.org/10.1080/16583655.2018.1495869Aryl halidesreductive dechlorinationdehalorespirationDesulfitobacteriumDehalococcoides |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wael S. El-Sayed Saleh R. Al-Senani Yasser Elbahloul |
spellingShingle |
Wael S. El-Sayed Saleh R. Al-Senani Yasser Elbahloul Diversity of dehalorespiring bacteria and selective enrichment of aryl halides-dechlorinating consortium from sedimentary environment near an oil refinery Journal of Taibah University for Science Aryl halides reductive dechlorination dehalorespiration Desulfitobacterium Dehalococcoides |
author_facet |
Wael S. El-Sayed Saleh R. Al-Senani Yasser Elbahloul |
author_sort |
Wael S. El-Sayed |
title |
Diversity of dehalorespiring bacteria and selective enrichment of aryl halides-dechlorinating consortium from sedimentary environment near an oil refinery |
title_short |
Diversity of dehalorespiring bacteria and selective enrichment of aryl halides-dechlorinating consortium from sedimentary environment near an oil refinery |
title_full |
Diversity of dehalorespiring bacteria and selective enrichment of aryl halides-dechlorinating consortium from sedimentary environment near an oil refinery |
title_fullStr |
Diversity of dehalorespiring bacteria and selective enrichment of aryl halides-dechlorinating consortium from sedimentary environment near an oil refinery |
title_full_unstemmed |
Diversity of dehalorespiring bacteria and selective enrichment of aryl halides-dechlorinating consortium from sedimentary environment near an oil refinery |
title_sort |
diversity of dehalorespiring bacteria and selective enrichment of aryl halides-dechlorinating consortium from sedimentary environment near an oil refinery |
publisher |
Taylor & Francis Group |
series |
Journal of Taibah University for Science |
issn |
1658-3655 |
publishDate |
2018-11-01 |
description |
This study demonstrated the potential for reductive dechlorination of aryl halides in a sedimentary environment by naturally occurring bacteria. A laboratory sediment microcosm and highly enriched, stable dechlorinating cultures were established using hydrogen as electron donor and 2,3-dichlorophenol (2,3DCP), monochlorophenol (MCP) and dichlorobenzene (DCB) as electron acceptors. 16S rRNA gene sequencing of dominant DGGE bands assigned detected phylotypes in chlorophenols (CPs) amended sediment microcosms to the genera Anaerospora, Pseudomonas, Desulfitobacterium, Clostridium, Mycobacterium, and Peptoclostridium, beside uncultured marine Bacterioplankton, Halochromatium and Sedimentibacter. Chlorobenzene (CB) amended sediments showed the same community in addition to Bacillus sp. Major operational taxonomic units (OTUs) in CPs enrichment cultures were assigned to Anaerospora hongkongensis, Pseudomonas stutzeri, Pseudomonas pseudoalcaligenes, Clostridium sp., Desulfitobacterium dichloroeliminans, beside unidentified marine bacterioplankton. Enrichment on DCB resulted in predominance of the same populations in addition to Peptoclostridium sp. and Dehalococcoides mccartyi. Dechlorination in enrichment cultures was mainly assigned to Desulfitobacterium and Dehalococcoides, which are both known for their ability to couple dechlorination to growth in a halorespiration way. Enrichment cultures containing Desulfitobacterium showed ortho dechlorination activity of 2,3DCP to 2 chlorophenol (2CP) and 3 chlorophenol (3CP). These results provided compelling evidence that sedimentary environments in the vicinity of oil refineries do harbor dehalorespiring bacteria capable of reductive dechlorination of various aryl halides pollutants. |
topic |
Aryl halides reductive dechlorination dehalorespiration Desulfitobacterium Dehalococcoides |
url |
http://dx.doi.org/10.1080/16583655.2018.1495869 |
work_keys_str_mv |
AT waelselsayed diversityofdehalorespiringbacteriaandselectiveenrichmentofarylhalidesdechlorinatingconsortiumfromsedimentaryenvironmentnearanoilrefinery AT salehralsenani diversityofdehalorespiringbacteriaandselectiveenrichmentofarylhalidesdechlorinatingconsortiumfromsedimentaryenvironmentnearanoilrefinery AT yasserelbahloul diversityofdehalorespiringbacteriaandselectiveenrichmentofarylhalidesdechlorinatingconsortiumfromsedimentaryenvironmentnearanoilrefinery |
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1725300128536854528 |