Ordered patterning of the sensory system is susceptible to stochastic features of gene expression
Sensory neuron numbers and positions are precisely organized to accurately map environmental signals in the brain. This precision emerges from biochemical processes within and between cells that are inherently stochastic. We investigated impact of stochastic gene expression on pattern formation, foc...
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doaj-6db9f188efc343ad947952776882d9992021-05-05T20:52:10ZengeLife Sciences Publications LtdeLife2050-084X2020-02-01910.7554/eLife.53638Ordered patterning of the sensory system is susceptible to stochastic features of gene expressionRitika Giri0https://orcid.org/0000-0001-8838-0818Dimitrios K Papadopoulos1Diana M Posadas2Hemanth K Potluri3Pavel Tomancak4https://orcid.org/0000-0002-2222-9370Madhav Mani5Richard W Carthew6https://orcid.org/0000-0003-0343-0156Department of Molecular Biosciences, Northwestern University, Evanston, United States; NSF-Simons Center for Quantitative Biology, Northwestern University, Evanston, United StatesMax Planck Institute of Cell Biology and Genetics, Dresden, GermanyDepartment of Molecular Biosciences, Northwestern University, Evanston, United StatesDepartment of Molecular Biosciences, Northwestern University, Evanston, United StatesMax Planck Institute of Cell Biology and Genetics, Dresden, GermanyDepartment of Molecular Biosciences, Northwestern University, Evanston, United States; NSF-Simons Center for Quantitative Biology, Northwestern University, Evanston, United States; Department of Engineering Sciences and Applied Mathematics, Northwestern University, Evanston, United StatesDepartment of Molecular Biosciences, Northwestern University, Evanston, United States; NSF-Simons Center for Quantitative Biology, Northwestern University, Evanston, United StatesSensory neuron numbers and positions are precisely organized to accurately map environmental signals in the brain. This precision emerges from biochemical processes within and between cells that are inherently stochastic. We investigated impact of stochastic gene expression on pattern formation, focusing on senseless (sens), a key determinant of sensory fate in Drosophila. Perturbing microRNA regulation or genomic location of sens produced distinct noise signatures. Noise was greatly enhanced when both sens alleles were present in homologous loci such that each allele was regulated in trans by the other allele. This led to disordered patterning. In contrast, loss of microRNA repression of sens increased protein abundance but not sensory pattern disorder. This suggests that gene expression stochasticity is a critical feature that must be constrained during development to allow rapid yet accurate cell fate resolution.https://elifesciences.org/articles/53638stochasticitygene expressionsensory developmentmicroRNAstransvection |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ritika Giri Dimitrios K Papadopoulos Diana M Posadas Hemanth K Potluri Pavel Tomancak Madhav Mani Richard W Carthew |
spellingShingle |
Ritika Giri Dimitrios K Papadopoulos Diana M Posadas Hemanth K Potluri Pavel Tomancak Madhav Mani Richard W Carthew Ordered patterning of the sensory system is susceptible to stochastic features of gene expression eLife stochasticity gene expression sensory development microRNAs transvection |
author_facet |
Ritika Giri Dimitrios K Papadopoulos Diana M Posadas Hemanth K Potluri Pavel Tomancak Madhav Mani Richard W Carthew |
author_sort |
Ritika Giri |
title |
Ordered patterning of the sensory system is susceptible to stochastic features of gene expression |
title_short |
Ordered patterning of the sensory system is susceptible to stochastic features of gene expression |
title_full |
Ordered patterning of the sensory system is susceptible to stochastic features of gene expression |
title_fullStr |
Ordered patterning of the sensory system is susceptible to stochastic features of gene expression |
title_full_unstemmed |
Ordered patterning of the sensory system is susceptible to stochastic features of gene expression |
title_sort |
ordered patterning of the sensory system is susceptible to stochastic features of gene expression |
publisher |
eLife Sciences Publications Ltd |
series |
eLife |
issn |
2050-084X |
publishDate |
2020-02-01 |
description |
Sensory neuron numbers and positions are precisely organized to accurately map environmental signals in the brain. This precision emerges from biochemical processes within and between cells that are inherently stochastic. We investigated impact of stochastic gene expression on pattern formation, focusing on senseless (sens), a key determinant of sensory fate in Drosophila. Perturbing microRNA regulation or genomic location of sens produced distinct noise signatures. Noise was greatly enhanced when both sens alleles were present in homologous loci such that each allele was regulated in trans by the other allele. This led to disordered patterning. In contrast, loss of microRNA repression of sens increased protein abundance but not sensory pattern disorder. This suggests that gene expression stochasticity is a critical feature that must be constrained during development to allow rapid yet accurate cell fate resolution. |
topic |
stochasticity gene expression sensory development microRNAs transvection |
url |
https://elifesciences.org/articles/53638 |
work_keys_str_mv |
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