Gut microbiota of patients with different subtypes of gastric cancer and gastrointestinal stromal tumors
Abstract Background Gastric adenocarcinoma is associated with H. pylori infection and inflammation that can result in the dysbiosis of gastric microbiota. The association of intestinal microbiota with gastric adenocarcinoma subtypes or with gastric gastrointestinal stromal tumors (GIST) is however n...
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doaj-6dbd39c97cbe44fda1546f2b84f3d1692021-02-21T12:23:04ZengBMCGut Pathogens1757-47492021-02-011311910.1186/s13099-021-00403-xGut microbiota of patients with different subtypes of gastric cancer and gastrointestinal stromal tumorsVirinder Sarhadi0Binu Mathew1Arto Kokkola2Tiina Karla3Milja Tikkanen4Hilpi Rautelin5Leo Lahti6Pauli Puolakkainen7Sakari Knuutila8Faculty of Medicine, Department of Pathology, University of HelsinkiDepartment of Computing, University of TurkuThe HUCH Gastrointestinal Clinic, University Central Hospital of HelsinkiThermo Fisher ScientificThermo Fisher ScientificDepartment of Medical Sciences, Clinical Microbiology, Uppsala UniversityDepartment of Computing, University of TurkuThe HUCH Gastrointestinal Clinic, University Central Hospital of HelsinkiFaculty of Medicine, Department of Pathology, University of HelsinkiAbstract Background Gastric adenocarcinoma is associated with H. pylori infection and inflammation that can result in the dysbiosis of gastric microbiota. The association of intestinal microbiota with gastric adenocarcinoma subtypes or with gastric gastrointestinal stromal tumors (GIST) is however not well known. Therefore, we performed 16S rRNA gene sequencing on DNA isolated from stool samples of Finnish patients and controls to study differences in microbiota among different histological subtypes of gastric adenocarcinoma, gastric GIST and healthy controls. Results We found that gut microbiota alpha diversity was lowest in diffuse adenocarcinoma patients, followed by intestinal type and GIST patients, although the differences were not significant compared to controls. Beta-diversity analysis however showed significant differences in microbiota composition for all subtypes compared to controls. Significantly higher abundance of Enterobacteriaceae was observed in both adenocarcinoma subtypes, whereas lower abundance of Bifidobacteriaceae was seen only in diffuse adenocarcinoma and of Oscillibacter in intestinal adenocarcinoma. Both GIST and adenocarcinoma patients had higher abundance of Enterobacteriaceae and lower abundance of Lactobacillaceae and Oscillibacter while lower abundance of Lachnoclostridium, Bifidobacterium, Parabacteroides and Barnesiella was seen only in the adenocarcinoma patients. Conclusions Our analysis shows association of higher Enterobacteriaceae abundance with all types of gastric tumors. Therefore it could be potentially useful as a marker of gastric malignancies. Lower gut microbiota diversity might be indicative of poorly differentiated, invasive, advanced or aggressive tumors and could possibly be a prognostic marker for gastric tumors.https://doi.org/10.1186/s13099-021-00403-xDiffuse gastric adenocarcinomaIntestinal gastric adenocarcinomaGISTGut microbiota |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Virinder Sarhadi Binu Mathew Arto Kokkola Tiina Karla Milja Tikkanen Hilpi Rautelin Leo Lahti Pauli Puolakkainen Sakari Knuutila |
spellingShingle |
Virinder Sarhadi Binu Mathew Arto Kokkola Tiina Karla Milja Tikkanen Hilpi Rautelin Leo Lahti Pauli Puolakkainen Sakari Knuutila Gut microbiota of patients with different subtypes of gastric cancer and gastrointestinal stromal tumors Gut Pathogens Diffuse gastric adenocarcinoma Intestinal gastric adenocarcinoma GIST Gut microbiota |
author_facet |
Virinder Sarhadi Binu Mathew Arto Kokkola Tiina Karla Milja Tikkanen Hilpi Rautelin Leo Lahti Pauli Puolakkainen Sakari Knuutila |
author_sort |
Virinder Sarhadi |
title |
Gut microbiota of patients with different subtypes of gastric cancer and gastrointestinal stromal tumors |
title_short |
Gut microbiota of patients with different subtypes of gastric cancer and gastrointestinal stromal tumors |
title_full |
Gut microbiota of patients with different subtypes of gastric cancer and gastrointestinal stromal tumors |
title_fullStr |
Gut microbiota of patients with different subtypes of gastric cancer and gastrointestinal stromal tumors |
title_full_unstemmed |
Gut microbiota of patients with different subtypes of gastric cancer and gastrointestinal stromal tumors |
title_sort |
gut microbiota of patients with different subtypes of gastric cancer and gastrointestinal stromal tumors |
publisher |
BMC |
series |
Gut Pathogens |
issn |
1757-4749 |
publishDate |
2021-02-01 |
description |
Abstract Background Gastric adenocarcinoma is associated with H. pylori infection and inflammation that can result in the dysbiosis of gastric microbiota. The association of intestinal microbiota with gastric adenocarcinoma subtypes or with gastric gastrointestinal stromal tumors (GIST) is however not well known. Therefore, we performed 16S rRNA gene sequencing on DNA isolated from stool samples of Finnish patients and controls to study differences in microbiota among different histological subtypes of gastric adenocarcinoma, gastric GIST and healthy controls. Results We found that gut microbiota alpha diversity was lowest in diffuse adenocarcinoma patients, followed by intestinal type and GIST patients, although the differences were not significant compared to controls. Beta-diversity analysis however showed significant differences in microbiota composition for all subtypes compared to controls. Significantly higher abundance of Enterobacteriaceae was observed in both adenocarcinoma subtypes, whereas lower abundance of Bifidobacteriaceae was seen only in diffuse adenocarcinoma and of Oscillibacter in intestinal adenocarcinoma. Both GIST and adenocarcinoma patients had higher abundance of Enterobacteriaceae and lower abundance of Lactobacillaceae and Oscillibacter while lower abundance of Lachnoclostridium, Bifidobacterium, Parabacteroides and Barnesiella was seen only in the adenocarcinoma patients. Conclusions Our analysis shows association of higher Enterobacteriaceae abundance with all types of gastric tumors. Therefore it could be potentially useful as a marker of gastric malignancies. Lower gut microbiota diversity might be indicative of poorly differentiated, invasive, advanced or aggressive tumors and could possibly be a prognostic marker for gastric tumors. |
topic |
Diffuse gastric adenocarcinoma Intestinal gastric adenocarcinoma GIST Gut microbiota |
url |
https://doi.org/10.1186/s13099-021-00403-x |
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