Pathological Study of Pigmented Dermatofibrosarcoma Protuberans
In this paper we performed morphological studies of Bednar tumor, which is a rare pigmented variant of dermatofibrosarcoma protuberans (DFSP) for evidence of histogenetic origin. Presence of melanosome-containing cells is the only histologic criterion of Bednar tumor, which is different from the or...
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doaj-6dccf31c72e34fa1bc12e466b10df8842021-08-13T09:42:12ZengMahidol UniversitySiriraj Medical Journal2228-80822020-08-01538Pathological Study of Pigmented Dermatofibrosarcoma ProtuberansMongkol Uiprasertkul0Punkae Mahaisavariya1Vanneet Khawcharoenporn2Nusara Wongvisitsak3Department of Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700Department of Dermatology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700Department of Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700Department of Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700 In this paper we performed morphological studies of Bednar tumor, which is a rare pigmented variant of dermatofibrosarcoma protuberans (DFSP) for evidence of histogenetic origin. Presence of melanosome-containing cells is the only histologic criterion of Bednar tumor, which is different from the ordinary DFSP. Immunohistochemical and ultrastructural examinations revealed that the melanosome-containing cells gave positive reactivity to antibodies to vimentin and S-100 protein, but negative to HMB-45, CD38, Factor VIII, NSE, and KP-1. The melanosomes are in Stage II, III, and IV, suggesting that the pigment was produced within the cell rather than phagocytosed from other cells. The rest of the tumor had similar pattern of immunoreactivity to DFSP such as positivity for CD34 and vimentin, indicating that Bednar tumor and DFSP originated from the same cell line. The ultrastructural feature of non-pigmented cells was similar to perineural cells in the first group and similar to fibroblasts in the second group. DNA flow cytometry in our cases revealed diploid cells with low to intermediate S-phase fraction and no aneuploid cells which were reported in a previous study. https://he02.tci-thaijo.org/index.php/sirirajmedj/article/view/244991Pigmented dermatofibrosarcoma protuberansBednarCD 34UltrastructureDNA flow cytometry |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mongkol Uiprasertkul Punkae Mahaisavariya Vanneet Khawcharoenporn Nusara Wongvisitsak |
spellingShingle |
Mongkol Uiprasertkul Punkae Mahaisavariya Vanneet Khawcharoenporn Nusara Wongvisitsak Pathological Study of Pigmented Dermatofibrosarcoma Protuberans Siriraj Medical Journal Pigmented dermatofibrosarcoma protuberans Bednar CD 34 Ultrastructure DNA flow cytometry |
author_facet |
Mongkol Uiprasertkul Punkae Mahaisavariya Vanneet Khawcharoenporn Nusara Wongvisitsak |
author_sort |
Mongkol Uiprasertkul |
title |
Pathological Study of Pigmented Dermatofibrosarcoma Protuberans |
title_short |
Pathological Study of Pigmented Dermatofibrosarcoma Protuberans |
title_full |
Pathological Study of Pigmented Dermatofibrosarcoma Protuberans |
title_fullStr |
Pathological Study of Pigmented Dermatofibrosarcoma Protuberans |
title_full_unstemmed |
Pathological Study of Pigmented Dermatofibrosarcoma Protuberans |
title_sort |
pathological study of pigmented dermatofibrosarcoma protuberans |
publisher |
Mahidol University |
series |
Siriraj Medical Journal |
issn |
2228-8082 |
publishDate |
2020-08-01 |
description |
In this paper we performed morphological studies of Bednar tumor, which is a rare pigmented variant of dermatofibrosarcoma protuberans (DFSP) for evidence of histogenetic origin. Presence of melanosome-containing cells is the only histologic criterion of Bednar tumor, which is different from the ordinary DFSP. Immunohistochemical and ultrastructural examinations revealed that the melanosome-containing cells gave positive reactivity to antibodies to vimentin and S-100 protein, but negative to HMB-45, CD38, Factor VIII, NSE, and KP-1. The melanosomes are in Stage II, III, and IV, suggesting that the pigment was produced within the cell rather than phagocytosed from other cells. The rest of the tumor had similar pattern of immunoreactivity to DFSP such as positivity for CD34 and vimentin, indicating that Bednar tumor and DFSP originated from the same cell line. The ultrastructural feature of non-pigmented cells was similar to perineural cells in the first group and similar to fibroblasts in the second group. DNA flow cytometry in our cases revealed diploid cells with low to intermediate S-phase fraction and no aneuploid cells which were reported in a previous study.
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topic |
Pigmented dermatofibrosarcoma protuberans Bednar CD 34 Ultrastructure DNA flow cytometry |
url |
https://he02.tci-thaijo.org/index.php/sirirajmedj/article/view/244991 |
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