Role of cyclic nucleotide-gated channels in the modulation of mouse hippocampal neurogenesis.
Neural stem cells generate neurons in the hippocampal dentate gyrus in mammals, including humans, throughout adulthood. Adult hippocampal neurogenesis has been the focus of many studies due to its relevance in processes such as learning and memory and its documented impairment in some neurodegenerat...
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doaj-6ddb38f71d3e4d7fbc00357a3ff273e02020-11-25T02:52:57ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0188e7324610.1371/journal.pone.0073246Role of cyclic nucleotide-gated channels in the modulation of mouse hippocampal neurogenesis.Maria Vittoria PoddaRoberto PiacentiniSaviana Antonella BarbatiAlessia MastrodonatoDaniela PuzzoMarcello D'AscenzoLucia LeoneClaudio GrassiNeural stem cells generate neurons in the hippocampal dentate gyrus in mammals, including humans, throughout adulthood. Adult hippocampal neurogenesis has been the focus of many studies due to its relevance in processes such as learning and memory and its documented impairment in some neurodegenerative diseases. However, we are still far from having a complete picture of the mechanism regulating this process. Our study focused on the possible role of cyclic nucleotide-gated (CNG) channels. These voltage-independent channels activated by cyclic nucleotides, first described in retinal and olfactory receptors, have been receiving increasing attention for their involvement in several brain functions. Here we show that the rod-type, CNGA1, and olfactory-type, CNGA2, subunits are expressed in hippocampal neural stem cells in culture and in situ in the hippocampal neurogenic niche of adult mice. Pharmacological blockade of CNG channels did not affect cultured neural stem cell proliferation but reduced their differentiation towards the neuronal phenotype. The membrane permeant cGMP analogue, 8-Br-cGMP, enhanced neural stem cell differentiation to neurons and this effect was prevented by CNG channel blockade. In addition, patch-clamp recording from neuron-like differentiating neural stem cells revealed cGMP-activated currents attributable to ion flow through CNG channels. The current work provides novel insights into the role of CNG channels in promoting hippocampal neurogenesis, which may prove to be relevant for stem cell-based treatment of cognitive impairment and brain damage.http://europepmc.org/articles/PMC3750014?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Maria Vittoria Podda Roberto Piacentini Saviana Antonella Barbati Alessia Mastrodonato Daniela Puzzo Marcello D'Ascenzo Lucia Leone Claudio Grassi |
spellingShingle |
Maria Vittoria Podda Roberto Piacentini Saviana Antonella Barbati Alessia Mastrodonato Daniela Puzzo Marcello D'Ascenzo Lucia Leone Claudio Grassi Role of cyclic nucleotide-gated channels in the modulation of mouse hippocampal neurogenesis. PLoS ONE |
author_facet |
Maria Vittoria Podda Roberto Piacentini Saviana Antonella Barbati Alessia Mastrodonato Daniela Puzzo Marcello D'Ascenzo Lucia Leone Claudio Grassi |
author_sort |
Maria Vittoria Podda |
title |
Role of cyclic nucleotide-gated channels in the modulation of mouse hippocampal neurogenesis. |
title_short |
Role of cyclic nucleotide-gated channels in the modulation of mouse hippocampal neurogenesis. |
title_full |
Role of cyclic nucleotide-gated channels in the modulation of mouse hippocampal neurogenesis. |
title_fullStr |
Role of cyclic nucleotide-gated channels in the modulation of mouse hippocampal neurogenesis. |
title_full_unstemmed |
Role of cyclic nucleotide-gated channels in the modulation of mouse hippocampal neurogenesis. |
title_sort |
role of cyclic nucleotide-gated channels in the modulation of mouse hippocampal neurogenesis. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2013-01-01 |
description |
Neural stem cells generate neurons in the hippocampal dentate gyrus in mammals, including humans, throughout adulthood. Adult hippocampal neurogenesis has been the focus of many studies due to its relevance in processes such as learning and memory and its documented impairment in some neurodegenerative diseases. However, we are still far from having a complete picture of the mechanism regulating this process. Our study focused on the possible role of cyclic nucleotide-gated (CNG) channels. These voltage-independent channels activated by cyclic nucleotides, first described in retinal and olfactory receptors, have been receiving increasing attention for their involvement in several brain functions. Here we show that the rod-type, CNGA1, and olfactory-type, CNGA2, subunits are expressed in hippocampal neural stem cells in culture and in situ in the hippocampal neurogenic niche of adult mice. Pharmacological blockade of CNG channels did not affect cultured neural stem cell proliferation but reduced their differentiation towards the neuronal phenotype. The membrane permeant cGMP analogue, 8-Br-cGMP, enhanced neural stem cell differentiation to neurons and this effect was prevented by CNG channel blockade. In addition, patch-clamp recording from neuron-like differentiating neural stem cells revealed cGMP-activated currents attributable to ion flow through CNG channels. The current work provides novel insights into the role of CNG channels in promoting hippocampal neurogenesis, which may prove to be relevant for stem cell-based treatment of cognitive impairment and brain damage. |
url |
http://europepmc.org/articles/PMC3750014?pdf=render |
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