Glycosylation Modulates Plasma Membrane Trafficking of CD24 in Breast Cancer Cells
In breast cancer, expression of Cluster of Differentiation 24 (CD24), a small GPI-anchored glycoprotein at the cell periphery, is associated with metastasis and immune escape, while its absence is associated with tumor-initiating capacity. Since the mechanism of CD24 sorting is unknown, we investiga...
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doaj-6dec006e09cb4a30b16910538fd614f02021-08-06T15:25:44ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-07-01228165816510.3390/ijms22158165Glycosylation Modulates Plasma Membrane Trafficking of CD24 in Breast Cancer CellsAmanda Chantziou0Kostas Theodorakis1Hara Polioudaki2Eelco de Bree3Marilena Kampa4Dimitris Mavroudis5Elias Castanas6Panayiotis A. Theodoropoulos7Laboratory of Biochemistry, School of Medicine, University of Crete, 70013 Heraklion, GreeceInstitute of Molecular Biology & Biotechnology—FoRTH, Heraklion, 70013 Heraklion, GreeceLaboratory of Biochemistry, School of Medicine, University of Crete, 70013 Heraklion, GreeceDepartment of Surgical Oncology, University General Hospital of Heraklion, 70013 Heraklion, GreeceLaboratory of Experimental Endocrinology, School of Medicine, University of Crete, 70013 Heraklion, GreeceDepartment of Medical Oncology, University General Hospital of Heraklion, 70013 Heraklion, GreeceLaboratory of Experimental Endocrinology, School of Medicine, University of Crete, 70013 Heraklion, GreeceLaboratory of Biochemistry, School of Medicine, University of Crete, 70013 Heraklion, GreeceIn breast cancer, expression of Cluster of Differentiation 24 (CD24), a small GPI-anchored glycoprotein at the cell periphery, is associated with metastasis and immune escape, while its absence is associated with tumor-initiating capacity. Since the mechanism of CD24 sorting is unknown, we investigated the role of glycosylation in the subcellular localization of CD24. Expression and localization of wild type N36- and/or N52-mutated CD24 were analyzed using immunofluorescence in luminal (MCF-7) and basal B (MDA-MB-231 and Hs578T) breast cancer cells lines, as well as HEK293T cells. Endogenous and exogenously expressed wild type and mutated CD24 were found localized at the plasma membrane and the cytoplasm, but not the nucleoplasm. The cell lines showed different kinetics for the sorting of CD24 through the secretory/endocytic pathway. N-glycosylation, especially at N52, and its processing in the Golgi were critical for the sorting and expression of CD24 at the plasma membrane of HEK293T and basal B type cells, but not of MCF-7 cells. In conclusion, our study highlights the contribution of N-glycosylation for the subcellular localization of CD24. Aberrant N-glycosylation at N52 of CD24 could account for the lack of CD24 expression at the cell surface of basal B breast cancer cells.https://www.mdpi.com/1422-0067/22/15/8165CD24glycosylationbreast cancerluminal and basal B cell linesendocytic sorting |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Amanda Chantziou Kostas Theodorakis Hara Polioudaki Eelco de Bree Marilena Kampa Dimitris Mavroudis Elias Castanas Panayiotis A. Theodoropoulos |
spellingShingle |
Amanda Chantziou Kostas Theodorakis Hara Polioudaki Eelco de Bree Marilena Kampa Dimitris Mavroudis Elias Castanas Panayiotis A. Theodoropoulos Glycosylation Modulates Plasma Membrane Trafficking of CD24 in Breast Cancer Cells International Journal of Molecular Sciences CD24 glycosylation breast cancer luminal and basal B cell lines endocytic sorting |
author_facet |
Amanda Chantziou Kostas Theodorakis Hara Polioudaki Eelco de Bree Marilena Kampa Dimitris Mavroudis Elias Castanas Panayiotis A. Theodoropoulos |
author_sort |
Amanda Chantziou |
title |
Glycosylation Modulates Plasma Membrane Trafficking of CD24 in Breast Cancer Cells |
title_short |
Glycosylation Modulates Plasma Membrane Trafficking of CD24 in Breast Cancer Cells |
title_full |
Glycosylation Modulates Plasma Membrane Trafficking of CD24 in Breast Cancer Cells |
title_fullStr |
Glycosylation Modulates Plasma Membrane Trafficking of CD24 in Breast Cancer Cells |
title_full_unstemmed |
Glycosylation Modulates Plasma Membrane Trafficking of CD24 in Breast Cancer Cells |
title_sort |
glycosylation modulates plasma membrane trafficking of cd24 in breast cancer cells |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2021-07-01 |
description |
In breast cancer, expression of Cluster of Differentiation 24 (CD24), a small GPI-anchored glycoprotein at the cell periphery, is associated with metastasis and immune escape, while its absence is associated with tumor-initiating capacity. Since the mechanism of CD24 sorting is unknown, we investigated the role of glycosylation in the subcellular localization of CD24. Expression and localization of wild type N36- and/or N52-mutated CD24 were analyzed using immunofluorescence in luminal (MCF-7) and basal B (MDA-MB-231 and Hs578T) breast cancer cells lines, as well as HEK293T cells. Endogenous and exogenously expressed wild type and mutated CD24 were found localized at the plasma membrane and the cytoplasm, but not the nucleoplasm. The cell lines showed different kinetics for the sorting of CD24 through the secretory/endocytic pathway. N-glycosylation, especially at N52, and its processing in the Golgi were critical for the sorting and expression of CD24 at the plasma membrane of HEK293T and basal B type cells, but not of MCF-7 cells. In conclusion, our study highlights the contribution of N-glycosylation for the subcellular localization of CD24. Aberrant N-glycosylation at N52 of CD24 could account for the lack of CD24 expression at the cell surface of basal B breast cancer cells. |
topic |
CD24 glycosylation breast cancer luminal and basal B cell lines endocytic sorting |
url |
https://www.mdpi.com/1422-0067/22/15/8165 |
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