Antifungal activity and genetic diversity of selected Pseudomonas spp. from maize rhizosphere in Vojvodina

• Main Authors: Jošić Dragana, Pivić Radmila, Miladinović Miroslav, Starović Mira, Pavlović Snežana, Đurić Simonida, Jarak Mirjana
• Format: Article
• Language: English
• Published: Serbian Genetics Society 2012-01-01
• Series: Genetika
• Subjects: antifungal activity; ERIC; (GTG)5; PGPR; phenazines; Pseudomonas
• Online Access: http://www.doiserbia.nb.rs/img/doi/0534-0012/2012/0534-00121202377J.pdf

Antibiotic production by plant-associated microorganisms represents an environmentally compatible method of disease control in agriculture. However, a vide application of bacterial strains needs careful selection and genetic characterization. In this investigation, selected Pseudomonas strains were characterized by rep-PCR methods using ERIC and (GTG)5 primers, and partial 16S rDNA sequence analysis. None of strains produced homoserine lactones (C4, C6, C8) as quorum sensing signal molecules. Very poor production of phenazines and no significant fungal inhibition was observed for PS4 and PS6 strains. High amount of phenazines were produced by Pseudomonas sp. strain PS2, which inhibited mycelial growth of 10 phytopatogenic fungi in percent of 25 (Verticillium sp.) to 65 (Fusarium equiseti). Genetic characterization of the Pseudomonas sp. PS2 and evaluation of phenazines production, as the main trait for growth inhibition of phytopathogenic fungi, will allow its application as a biosafe PGPR for field experiments of plant disease control. [Projekat Ministarstva nauke Republike Srbije, br. III 46007: New indigenous bacterial isolates Lysobacter and Pseudomonas as an important sources of metabolites useful for biotechnology, plant growth stimulation and disease control: From isolates to inoculants]