Protein tyrosine phosphatase 1B regulates migration of ARPE-19 cells through EGFR/ERK signaling pathway
<b>AIM:</b> To evaluate whether protein tyrosine phosphatase 1B (PTP1B) contributed to initiate human retinal pigment epithelium cells (A)-19 migration and investigate the signaling pathways involved in this process.<b>METHODS:</b>ARPE-19 cells were cultured and treated with...
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doaj-6e146dfa48764fe3aaec16202d8bdd7e2020-11-24T23:10:29ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982015-10-018589189710.3980/j.issn.2222-3959.2015.05.07Protein tyrosine phosphatase 1B regulates migration of ARPE-19 cells through EGFR/ERK signaling pathwayZhao-Dong Du0Li-Ting Hu1Gui-Qiu Zhao2Qian Wang3Qiang Xu4Nan Jiang5Jing Lin6Department of Ophthalmology, Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong Province, ChinaDepartment of Ophthalmology, Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong Province, ChinaDepartment of Ophthalmology, Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong Province, ChinaDepartment of Ophthalmology, Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong Province, ChinaDepartment of Ophthalmology, Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong Province, ChinaDepartment of Ophthalmology, Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong Province, ChinaDepartment of Ophthalmology, Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong Province, China<b>AIM:</b> To evaluate whether protein tyrosine phosphatase 1B (PTP1B) contributed to initiate human retinal pigment epithelium cells (A)-19 migration and investigate the signaling pathways involved in this process.<b>METHODS:</b>ARPE-19 cells were cultured and treated with the siRNA-PTP1B. Expression of PTP1B was confirmed by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). AG1478 [a selective inhibitor of epidermal growth factor receptor (EGFR)] and PD98059 (a specific inhibitor of the activation of mitogen-activated protein kinase) were used to help to determine the PTP1B signaling mechanism. Western blot analysis verified expression of EGFR and extracellular signal-regulated kinase (ERK) in ARPE-19 cells. The effect of siRNA-PTP1B on cell differentiation was confirmed by immunostaining for α-smooth muscle actin (α-SMA) and qRT-PCR. Cell migration ability was analyzed by transwell chamber assay.<b>RESULTS:</b> The mRNA levels of PTP1B were reduced by siRNA-PTP1B as determined by qRT-PCR assay. SiRNA-PTP1B activated EGFR and ERK phosphorylation. α-SMA staining and qRT-PCR assay demonstrated that siRNA-PTP1B induced retinal pigment epithelium (RPE) cells to differentiate toward better contractility and motility. Transwell chamber assay proved that PTP1B inhibition improved migration activity of RPE cells. Treatment with AG1478 and PD98059 abolished siRNA-PTP1B-induced activation of EGFR and ERK, α-SMA expression and cell migration.<b>CONCLUSION:</b> PTP1B inhibition promoted myofibroblast differentiation and migration of ARPE-19 cells, and EGFR/ERK signaling pathway played important role in migration process.http://www.ijo.cn/en_publish/2015/5/20150507.pdfprotein tyrosine phosphatase 1Bretinal pigment epitheliumcell migrationepidermal growth factor receptorextracellular signal-regulated kinase |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhao-Dong Du Li-Ting Hu Gui-Qiu Zhao Qian Wang Qiang Xu Nan Jiang Jing Lin |
spellingShingle |
Zhao-Dong Du Li-Ting Hu Gui-Qiu Zhao Qian Wang Qiang Xu Nan Jiang Jing Lin Protein tyrosine phosphatase 1B regulates migration of ARPE-19 cells through EGFR/ERK signaling pathway International Journal of Ophthalmology protein tyrosine phosphatase 1B retinal pigment epithelium cell migration epidermal growth factor receptor extracellular signal-regulated kinase |
author_facet |
Zhao-Dong Du Li-Ting Hu Gui-Qiu Zhao Qian Wang Qiang Xu Nan Jiang Jing Lin |
author_sort |
Zhao-Dong Du |
title |
Protein tyrosine phosphatase 1B regulates migration of ARPE-19 cells through EGFR/ERK signaling pathway |
title_short |
Protein tyrosine phosphatase 1B regulates migration of ARPE-19 cells through EGFR/ERK signaling pathway |
title_full |
Protein tyrosine phosphatase 1B regulates migration of ARPE-19 cells through EGFR/ERK signaling pathway |
title_fullStr |
Protein tyrosine phosphatase 1B regulates migration of ARPE-19 cells through EGFR/ERK signaling pathway |
title_full_unstemmed |
Protein tyrosine phosphatase 1B regulates migration of ARPE-19 cells through EGFR/ERK signaling pathway |
title_sort |
protein tyrosine phosphatase 1b regulates migration of arpe-19 cells through egfr/erk signaling pathway |
publisher |
Press of International Journal of Ophthalmology (IJO PRESS) |
series |
International Journal of Ophthalmology |
issn |
2222-3959 2227-4898 |
publishDate |
2015-10-01 |
description |
<b>AIM:</b> To evaluate whether protein tyrosine phosphatase 1B (PTP1B) contributed to initiate human retinal pigment epithelium cells (A)-19 migration and investigate the signaling pathways involved in this process.<b>METHODS:</b>ARPE-19 cells were cultured and treated with the siRNA-PTP1B. Expression of PTP1B was confirmed by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). AG1478 [a selective inhibitor of epidermal growth factor receptor (EGFR)] and PD98059 (a specific inhibitor of the activation of mitogen-activated protein kinase) were used to help to determine the PTP1B signaling mechanism. Western blot analysis verified expression of EGFR and extracellular signal-regulated kinase (ERK) in ARPE-19 cells. The effect of siRNA-PTP1B on cell differentiation was confirmed by immunostaining for α-smooth muscle actin (α-SMA) and qRT-PCR. Cell migration ability was analyzed by transwell chamber assay.<b>RESULTS:</b> The mRNA levels of PTP1B were reduced by siRNA-PTP1B as determined by qRT-PCR assay. SiRNA-PTP1B activated EGFR and ERK phosphorylation. α-SMA staining and qRT-PCR assay demonstrated that siRNA-PTP1B induced retinal pigment epithelium (RPE) cells to differentiate toward better contractility and motility. Transwell chamber assay proved that PTP1B inhibition improved migration activity of RPE cells. Treatment with AG1478 and PD98059 abolished siRNA-PTP1B-induced activation of EGFR and ERK, α-SMA expression and cell migration.<b>CONCLUSION:</b> PTP1B inhibition promoted myofibroblast differentiation and migration of ARPE-19 cells, and EGFR/ERK signaling pathway played important role in migration process. |
topic |
protein tyrosine phosphatase 1B retinal pigment epithelium cell migration epidermal growth factor receptor extracellular signal-regulated kinase |
url |
http://www.ijo.cn/en_publish/2015/5/20150507.pdf |
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