De Novo Transcriptome Analysis of Plant Pathogenic Fungus Myrothecium roridum and Identification of Genes Associated with Trichothecene Mycotoxin Biosynthesis
Myrothecium roridum is a plant pathogenic fungus that infects different crops and decreases the yield of economical crops, including soybean, cotton, corn, pepper, and tomato. Until now, the pathogenic mechanism of M. roridum has remained unclear. Different types of trichothecene mycotoxins were iso...
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Language: | English |
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MDPI AG
2017-02-01
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Series: | International Journal of Molecular Sciences |
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Online Access: | http://www.mdpi.com/1422-0067/18/3/497 |
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doaj-6e199748ebe043659ccebfd997c04094 |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wei Ye Taomei Liu Muzi Zhu Weimin Zhang Haohua Li Zilei Huang Saini Li |
spellingShingle |
Wei Ye Taomei Liu Muzi Zhu Weimin Zhang Haohua Li Zilei Huang Saini Li De Novo Transcriptome Analysis of Plant Pathogenic Fungus Myrothecium roridum and Identification of Genes Associated with Trichothecene Mycotoxin Biosynthesis International Journal of Molecular Sciences Myrothecium roridum plant pathogenic fungus transcriptome trichothecene biosynthesis MAPK signal pathway |
author_facet |
Wei Ye Taomei Liu Muzi Zhu Weimin Zhang Haohua Li Zilei Huang Saini Li |
author_sort |
Wei Ye |
title |
De Novo Transcriptome Analysis of Plant Pathogenic Fungus Myrothecium roridum and Identification of Genes Associated with Trichothecene Mycotoxin Biosynthesis |
title_short |
De Novo Transcriptome Analysis of Plant Pathogenic Fungus Myrothecium roridum and Identification of Genes Associated with Trichothecene Mycotoxin Biosynthesis |
title_full |
De Novo Transcriptome Analysis of Plant Pathogenic Fungus Myrothecium roridum and Identification of Genes Associated with Trichothecene Mycotoxin Biosynthesis |
title_fullStr |
De Novo Transcriptome Analysis of Plant Pathogenic Fungus Myrothecium roridum and Identification of Genes Associated with Trichothecene Mycotoxin Biosynthesis |
title_full_unstemmed |
De Novo Transcriptome Analysis of Plant Pathogenic Fungus Myrothecium roridum and Identification of Genes Associated with Trichothecene Mycotoxin Biosynthesis |
title_sort |
de novo transcriptome analysis of plant pathogenic fungus myrothecium roridum and identification of genes associated with trichothecene mycotoxin biosynthesis |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2017-02-01 |
description |
Myrothecium roridum is a plant pathogenic fungus that infects different crops and decreases the yield of economical crops, including soybean, cotton, corn, pepper, and tomato. Until now, the pathogenic mechanism of M. roridum has remained unclear. Different types of trichothecene mycotoxins were isolated from M. roridum, and trichothecene was considered as a plant pathogenic factor of M. roridum. In this study, the transcriptome of M. roridum in different incubation durations was sequenced using an Illumina Hiseq 2000. A total of 35,485 transcripts and 25,996 unigenes for M. roridum were obtained from 8.0 Gb clean reads. The protein–protein network of the M. roridum transcriptome indicated that the mitogen-activated protein kinases signal pathway also played an important role in the pathogenicity of M. roridum. The genes related to trichothecene biosynthesis were annotated. The expression levels of these genes were also predicted and validated through quantitative real-time polymerase chain reaction. Tri5 gene encoding trichodiene synthase was cloned and expressed, and the purified trichodiene synthase was able to catalyze farnesyl pyrophosphate into different kinds of sesquiterpenoids.Tri4 and Tri11 genes were expressed in Escherichia coli, and their corresponding enzymatic properties were characterized. The phylogenetic tree of trichodiene synthase showed a great discrepancy between the trichodiene synthase from M. roridum and other species. Our study on the genes related to trichothecene biosynthesis establishes a foundation for the M. roridum hazard prevention, thus improving the yields of economical crops. |
topic |
Myrothecium roridum plant pathogenic fungus transcriptome trichothecene biosynthesis MAPK signal pathway |
url |
http://www.mdpi.com/1422-0067/18/3/497 |
work_keys_str_mv |
AT weiye denovotranscriptomeanalysisofplantpathogenicfungusmyrotheciumroridumandidentificationofgenesassociatedwithtrichothecenemycotoxinbiosynthesis AT taomeiliu denovotranscriptomeanalysisofplantpathogenicfungusmyrotheciumroridumandidentificationofgenesassociatedwithtrichothecenemycotoxinbiosynthesis AT muzizhu denovotranscriptomeanalysisofplantpathogenicfungusmyrotheciumroridumandidentificationofgenesassociatedwithtrichothecenemycotoxinbiosynthesis AT weiminzhang denovotranscriptomeanalysisofplantpathogenicfungusmyrotheciumroridumandidentificationofgenesassociatedwithtrichothecenemycotoxinbiosynthesis AT haohuali denovotranscriptomeanalysisofplantpathogenicfungusmyrotheciumroridumandidentificationofgenesassociatedwithtrichothecenemycotoxinbiosynthesis AT zileihuang denovotranscriptomeanalysisofplantpathogenicfungusmyrotheciumroridumandidentificationofgenesassociatedwithtrichothecenemycotoxinbiosynthesis AT sainili denovotranscriptomeanalysisofplantpathogenicfungusmyrotheciumroridumandidentificationofgenesassociatedwithtrichothecenemycotoxinbiosynthesis |
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spelling |
doaj-6e199748ebe043659ccebfd997c040942020-11-24T22:50:02ZengMDPI AGInternational Journal of Molecular Sciences1422-00672017-02-0118349710.3390/ijms18030497ijms18030497De Novo Transcriptome Analysis of Plant Pathogenic Fungus Myrothecium roridum and Identification of Genes Associated with Trichothecene Mycotoxin BiosynthesisWei Ye0Taomei Liu1Muzi Zhu2Weimin Zhang3Haohua Li4Zilei Huang5Saini Li6State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, No. 100 Xianlie Middle Road, Yuexiu District, Guangzhou 510070, ChinaState Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, No. 100 Xianlie Middle Road, Yuexiu District, Guangzhou 510070, ChinaState Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, No. 100 Xianlie Middle Road, Yuexiu District, Guangzhou 510070, ChinaState Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, No. 100 Xianlie Middle Road, Yuexiu District, Guangzhou 510070, ChinaState Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, No. 100 Xianlie Middle Road, Yuexiu District, Guangzhou 510070, ChinaState Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, No. 100 Xianlie Middle Road, Yuexiu District, Guangzhou 510070, ChinaState Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, No. 100 Xianlie Middle Road, Yuexiu District, Guangzhou 510070, ChinaMyrothecium roridum is a plant pathogenic fungus that infects different crops and decreases the yield of economical crops, including soybean, cotton, corn, pepper, and tomato. Until now, the pathogenic mechanism of M. roridum has remained unclear. Different types of trichothecene mycotoxins were isolated from M. roridum, and trichothecene was considered as a plant pathogenic factor of M. roridum. In this study, the transcriptome of M. roridum in different incubation durations was sequenced using an Illumina Hiseq 2000. A total of 35,485 transcripts and 25,996 unigenes for M. roridum were obtained from 8.0 Gb clean reads. The protein–protein network of the M. roridum transcriptome indicated that the mitogen-activated protein kinases signal pathway also played an important role in the pathogenicity of M. roridum. The genes related to trichothecene biosynthesis were annotated. The expression levels of these genes were also predicted and validated through quantitative real-time polymerase chain reaction. Tri5 gene encoding trichodiene synthase was cloned and expressed, and the purified trichodiene synthase was able to catalyze farnesyl pyrophosphate into different kinds of sesquiterpenoids.Tri4 and Tri11 genes were expressed in Escherichia coli, and their corresponding enzymatic properties were characterized. The phylogenetic tree of trichodiene synthase showed a great discrepancy between the trichodiene synthase from M. roridum and other species. Our study on the genes related to trichothecene biosynthesis establishes a foundation for the M. roridum hazard prevention, thus improving the yields of economical crops.http://www.mdpi.com/1422-0067/18/3/497Myrothecium roridumplant pathogenic fungustranscriptometrichothecene biosynthesisMAPK signal pathway |