Comparative Effects of Pterostilbene and Its Parent Compound Resveratrol on Oxidative Stress and Inflammation in Steatohepatitis Induced by High-Fat High-Fructose Feeding
Different studies have revealed that oxidative stress and inflammation are crucial in NAFLD (Non-alcoholic fatty liver disease). The aim of this study is to analyze whether pterostilbene and resveratrol are able to either avoid or delay the progression of non-alcoholic liver steatosis towards steato...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2020-10-01
|
Series: | Antioxidants |
Subjects: | |
Online Access: | https://www.mdpi.com/2076-3921/9/11/1042 |
id |
doaj-6e215d5baf7f47b59931f37dfb1152c2 |
---|---|
record_format |
Article |
spelling |
doaj-6e215d5baf7f47b59931f37dfb1152c22020-11-25T03:35:04ZengMDPI AGAntioxidants2076-39212020-10-0191042104210.3390/antiox9111042Comparative Effects of Pterostilbene and Its Parent Compound Resveratrol on Oxidative Stress and Inflammation in Steatohepatitis Induced by High-Fat High-Fructose FeedingSaioa Gómez-Zorita0Maitane González-Arceo1Jenifer Trepiana2Leixuri Aguirre3Ana B Crujeiras4Esperanza Irles5Nerea Segues6Luis Bujanda7María Puy Portillo8Nutrition and Obesity group, Department of Nutrition and Food Science, Faculty of Pharmacy, University of the Basque Country (UPV/EHU), Lucio Lascaray Research Centre, 01006 Vitoria-Gasteiz, SpainNutrition and Obesity group, Department of Nutrition and Food Science, Faculty of Pharmacy, University of the Basque Country (UPV/EHU), Lucio Lascaray Research Centre, 01006 Vitoria-Gasteiz, SpainNutrition and Obesity group, Department of Nutrition and Food Science, Faculty of Pharmacy, University of the Basque Country (UPV/EHU), Lucio Lascaray Research Centre, 01006 Vitoria-Gasteiz, SpainNutrition and Obesity group, Department of Nutrition and Food Science, Faculty of Pharmacy, University of the Basque Country (UPV/EHU), Lucio Lascaray Research Centre, 01006 Vitoria-Gasteiz, SpainCIBERobn Physiopathology of Obesity and Nutrition, Institute of Health Carlos III (ISCIII), 01006 Vitoria-Gasteiz, SpainNutrition and Obesity group, Department of Nutrition and Food Science, Faculty of Pharmacy, University of the Basque Country (UPV/EHU), Lucio Lascaray Research Centre, 01006 Vitoria-Gasteiz, SpainDepartment of Gastroenterology, University of the Basque Country (UPV/EHU), Donostia Hospital, 00685 San Sebastián, SpainDepartment of Gastroenterology, University of the Basque Country (UPV/EHU), Donostia Hospital, 00685 San Sebastián, SpainNutrition and Obesity group, Department of Nutrition and Food Science, Faculty of Pharmacy, University of the Basque Country (UPV/EHU), Lucio Lascaray Research Centre, 01006 Vitoria-Gasteiz, SpainDifferent studies have revealed that oxidative stress and inflammation are crucial in NAFLD (Non-alcoholic fatty liver disease). The aim of this study is to analyze whether pterostilbene and resveratrol are able to either avoid or delay the progression of non-alcoholic liver steatosis towards steatohepatitis. This has been performed by examining their effects on oxidative stress, inflammation, fibrosis and pre-carcinogenic stages. Rats were distributed into five experimental groups and were fed with either a standard diet or a high-fat high-fructose diet, supplemented or not with pterostilbene (15 or 30 mg/kg/d) or resveratrol (30 mg/kg/d), for 8 weeks. Liver histological analysis was carried out by haematoxylin–eosin staining. Serum and hepatic oxidative stress-related parameters were assessed using spectrophotometry, and the expression of genes related to inflammation, fibrosis and cancer by qRT-PCR. The dietary model used in this study led to the development of steatohepatitis, where rats displayed oxidative stress, inflammation and ballooning, although not fibrosis. It also modified the expression of hepatocarcinoma-related genes. The results show, for the first time, that pterostilbene was able to partially prevent these alterations, with the exception of changes in hepatocarcinoma-related genes, mainly at 30 mg/kg/d. Pterostilbene was more effective than its parent compound resveratrol, probably due to its high bioavailability and higher anti-oxidant and anti-inflammatory activities, attributable to its different chemical structure.https://www.mdpi.com/2076-3921/9/11/1042pterostilbeneresveratrol(poly)phenolsliver steatosisliver steatohepatitishepatocarcinoma |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Saioa Gómez-Zorita Maitane González-Arceo Jenifer Trepiana Leixuri Aguirre Ana B Crujeiras Esperanza Irles Nerea Segues Luis Bujanda María Puy Portillo |
spellingShingle |
Saioa Gómez-Zorita Maitane González-Arceo Jenifer Trepiana Leixuri Aguirre Ana B Crujeiras Esperanza Irles Nerea Segues Luis Bujanda María Puy Portillo Comparative Effects of Pterostilbene and Its Parent Compound Resveratrol on Oxidative Stress and Inflammation in Steatohepatitis Induced by High-Fat High-Fructose Feeding Antioxidants pterostilbene resveratrol (poly)phenols liver steatosis liver steatohepatitis hepatocarcinoma |
author_facet |
Saioa Gómez-Zorita Maitane González-Arceo Jenifer Trepiana Leixuri Aguirre Ana B Crujeiras Esperanza Irles Nerea Segues Luis Bujanda María Puy Portillo |
author_sort |
Saioa Gómez-Zorita |
title |
Comparative Effects of Pterostilbene and Its Parent Compound Resveratrol on Oxidative Stress and Inflammation in Steatohepatitis Induced by High-Fat High-Fructose Feeding |
title_short |
Comparative Effects of Pterostilbene and Its Parent Compound Resveratrol on Oxidative Stress and Inflammation in Steatohepatitis Induced by High-Fat High-Fructose Feeding |
title_full |
Comparative Effects of Pterostilbene and Its Parent Compound Resveratrol on Oxidative Stress and Inflammation in Steatohepatitis Induced by High-Fat High-Fructose Feeding |
title_fullStr |
Comparative Effects of Pterostilbene and Its Parent Compound Resveratrol on Oxidative Stress and Inflammation in Steatohepatitis Induced by High-Fat High-Fructose Feeding |
title_full_unstemmed |
Comparative Effects of Pterostilbene and Its Parent Compound Resveratrol on Oxidative Stress and Inflammation in Steatohepatitis Induced by High-Fat High-Fructose Feeding |
title_sort |
comparative effects of pterostilbene and its parent compound resveratrol on oxidative stress and inflammation in steatohepatitis induced by high-fat high-fructose feeding |
publisher |
MDPI AG |
series |
Antioxidants |
issn |
2076-3921 |
publishDate |
2020-10-01 |
description |
Different studies have revealed that oxidative stress and inflammation are crucial in NAFLD (Non-alcoholic fatty liver disease). The aim of this study is to analyze whether pterostilbene and resveratrol are able to either avoid or delay the progression of non-alcoholic liver steatosis towards steatohepatitis. This has been performed by examining their effects on oxidative stress, inflammation, fibrosis and pre-carcinogenic stages. Rats were distributed into five experimental groups and were fed with either a standard diet or a high-fat high-fructose diet, supplemented or not with pterostilbene (15 or 30 mg/kg/d) or resveratrol (30 mg/kg/d), for 8 weeks. Liver histological analysis was carried out by haematoxylin–eosin staining. Serum and hepatic oxidative stress-related parameters were assessed using spectrophotometry, and the expression of genes related to inflammation, fibrosis and cancer by qRT-PCR. The dietary model used in this study led to the development of steatohepatitis, where rats displayed oxidative stress, inflammation and ballooning, although not fibrosis. It also modified the expression of hepatocarcinoma-related genes. The results show, for the first time, that pterostilbene was able to partially prevent these alterations, with the exception of changes in hepatocarcinoma-related genes, mainly at 30 mg/kg/d. Pterostilbene was more effective than its parent compound resveratrol, probably due to its high bioavailability and higher anti-oxidant and anti-inflammatory activities, attributable to its different chemical structure. |
topic |
pterostilbene resveratrol (poly)phenols liver steatosis liver steatohepatitis hepatocarcinoma |
url |
https://www.mdpi.com/2076-3921/9/11/1042 |
work_keys_str_mv |
AT saioagomezzorita comparativeeffectsofpterostilbeneanditsparentcompoundresveratrolonoxidativestressandinflammationinsteatohepatitisinducedbyhighfathighfructosefeeding AT maitanegonzalezarceo comparativeeffectsofpterostilbeneanditsparentcompoundresveratrolonoxidativestressandinflammationinsteatohepatitisinducedbyhighfathighfructosefeeding AT jenifertrepiana comparativeeffectsofpterostilbeneanditsparentcompoundresveratrolonoxidativestressandinflammationinsteatohepatitisinducedbyhighfathighfructosefeeding AT leixuriaguirre comparativeeffectsofpterostilbeneanditsparentcompoundresveratrolonoxidativestressandinflammationinsteatohepatitisinducedbyhighfathighfructosefeeding AT anabcrujeiras comparativeeffectsofpterostilbeneanditsparentcompoundresveratrolonoxidativestressandinflammationinsteatohepatitisinducedbyhighfathighfructosefeeding AT esperanzairles comparativeeffectsofpterostilbeneanditsparentcompoundresveratrolonoxidativestressandinflammationinsteatohepatitisinducedbyhighfathighfructosefeeding AT nereasegues comparativeeffectsofpterostilbeneanditsparentcompoundresveratrolonoxidativestressandinflammationinsteatohepatitisinducedbyhighfathighfructosefeeding AT luisbujanda comparativeeffectsofpterostilbeneanditsparentcompoundresveratrolonoxidativestressandinflammationinsteatohepatitisinducedbyhighfathighfructosefeeding AT mariapuyportillo comparativeeffectsofpterostilbeneanditsparentcompoundresveratrolonoxidativestressandinflammationinsteatohepatitisinducedbyhighfathighfructosefeeding |
_version_ |
1724555736115052544 |