Human papillomavirus type 18 oncoproteins exert their oncogenicity in esophageal and tongue squamous cell carcinoma cell lines distinctly
Abstract Background Increasing evidence indicates an etiological role of human papillomavirus (HPV) in head and neck cancers, particularly oropharyngeal squamous cell carcinoma (OPSCC). However, the association between HPV and other cancers, including esophageal and tongue remains unclear. This stud...
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doaj-6e3411ee9ab647b2b6c39c460de912e72020-12-13T12:40:01ZengBMCBMC Cancer1471-24072019-12-0119111210.1186/s12885-019-6413-7Human papillomavirus type 18 oncoproteins exert their oncogenicity in esophageal and tongue squamous cell carcinoma cell lines distinctlySiaw Shi Boon0Zigui Chen1Jintao Li2Karen Y. C. Lee3Liuyang Cai4Rugang Zhong5Paul K. S. Chan6Department of Microbiology, Faculty of Medicine, The Chinese University of Hong KongDepartment of Microbiology, Faculty of Medicine, The Chinese University of Hong KongBeijing Key Laboratory of Environmental and Viral Oncology, College of Life Science and Bio-engineering, Beijing University of TechnologyDepartment of Microbiology, Faculty of Medicine, The Chinese University of Hong KongDepartment of Microbiology, Faculty of Medicine, The Chinese University of Hong KongBeijing Key Laboratory of Environmental and Viral Oncology, College of Life Science and Bio-engineering, Beijing University of TechnologyDepartment of Microbiology, Faculty of Medicine, The Chinese University of Hong KongAbstract Background Increasing evidence indicates an etiological role of human papillomavirus (HPV) in head and neck cancers, particularly oropharyngeal squamous cell carcinoma (OPSCC). However, the association between HPV and other cancers, including esophageal and tongue remains unclear. This study delineated the molecular characteristics of HPV18 E6 and E7 in esophageal (EC109 and EC9706) and tongue (Tca83) cancer cell lines with reference to cervical cancer (HeLa). Methods We analysed the HPV transcription profiles of esophageal and tongue cancer cells through Next-generation RNA sequencing, and the role of HPV18 E6 and E7 in these cells was assessed via siRNA approach, Western blotting and immunofluorescence assays. Results Overall, the HPV transcription profiles of esophageal and tongue cancer cells mimicked that of cervical cancer cells, with notable disruption of E2, and expression of E6, spliced E6 (E6*), E7, E1 and L1 transcripts. As with cervical cancer cells, p53 and its downstream transactivation target, p21, were found to be the major targets of E6 in esophageal and tongue cancer cell lines. Intriguingly, E7 preferentially targeted p130 in the two esophageal cancer cell lines, instead of pRb as in cervical cancer. Tca83 exhibited an E7 to E6 transcript ratio comparable to HeLa (cervix), targeted the ERK1/2 and MMP2 pathways, and was dependent on E6 and E7 to survive and proliferate. In contrast, both the esophageal cancer cell lines were distinct from HeLa in these aspects. Conclusions This is the first study that delineates transcript expression and protein interaction of HPV18 E6 and E7 in esophageal and tongue cancer cell lines, suggesting that HPV plays a role in inducing these cancers, albeit via distinct pathways than those observed in cervical cancer.https://doi.org/10.1186/s12885-019-6413-7HPV18Esophageal squamous cell carcinomaTongue squamous cell carcinomaE6E7 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Siaw Shi Boon Zigui Chen Jintao Li Karen Y. C. Lee Liuyang Cai Rugang Zhong Paul K. S. Chan |
spellingShingle |
Siaw Shi Boon Zigui Chen Jintao Li Karen Y. C. Lee Liuyang Cai Rugang Zhong Paul K. S. Chan Human papillomavirus type 18 oncoproteins exert their oncogenicity in esophageal and tongue squamous cell carcinoma cell lines distinctly BMC Cancer HPV18 Esophageal squamous cell carcinoma Tongue squamous cell carcinoma E6 E7 |
author_facet |
Siaw Shi Boon Zigui Chen Jintao Li Karen Y. C. Lee Liuyang Cai Rugang Zhong Paul K. S. Chan |
author_sort |
Siaw Shi Boon |
title |
Human papillomavirus type 18 oncoproteins exert their oncogenicity in esophageal and tongue squamous cell carcinoma cell lines distinctly |
title_short |
Human papillomavirus type 18 oncoproteins exert their oncogenicity in esophageal and tongue squamous cell carcinoma cell lines distinctly |
title_full |
Human papillomavirus type 18 oncoproteins exert their oncogenicity in esophageal and tongue squamous cell carcinoma cell lines distinctly |
title_fullStr |
Human papillomavirus type 18 oncoproteins exert their oncogenicity in esophageal and tongue squamous cell carcinoma cell lines distinctly |
title_full_unstemmed |
Human papillomavirus type 18 oncoproteins exert their oncogenicity in esophageal and tongue squamous cell carcinoma cell lines distinctly |
title_sort |
human papillomavirus type 18 oncoproteins exert their oncogenicity in esophageal and tongue squamous cell carcinoma cell lines distinctly |
publisher |
BMC |
series |
BMC Cancer |
issn |
1471-2407 |
publishDate |
2019-12-01 |
description |
Abstract Background Increasing evidence indicates an etiological role of human papillomavirus (HPV) in head and neck cancers, particularly oropharyngeal squamous cell carcinoma (OPSCC). However, the association between HPV and other cancers, including esophageal and tongue remains unclear. This study delineated the molecular characteristics of HPV18 E6 and E7 in esophageal (EC109 and EC9706) and tongue (Tca83) cancer cell lines with reference to cervical cancer (HeLa). Methods We analysed the HPV transcription profiles of esophageal and tongue cancer cells through Next-generation RNA sequencing, and the role of HPV18 E6 and E7 in these cells was assessed via siRNA approach, Western blotting and immunofluorescence assays. Results Overall, the HPV transcription profiles of esophageal and tongue cancer cells mimicked that of cervical cancer cells, with notable disruption of E2, and expression of E6, spliced E6 (E6*), E7, E1 and L1 transcripts. As with cervical cancer cells, p53 and its downstream transactivation target, p21, were found to be the major targets of E6 in esophageal and tongue cancer cell lines. Intriguingly, E7 preferentially targeted p130 in the two esophageal cancer cell lines, instead of pRb as in cervical cancer. Tca83 exhibited an E7 to E6 transcript ratio comparable to HeLa (cervix), targeted the ERK1/2 and MMP2 pathways, and was dependent on E6 and E7 to survive and proliferate. In contrast, both the esophageal cancer cell lines were distinct from HeLa in these aspects. Conclusions This is the first study that delineates transcript expression and protein interaction of HPV18 E6 and E7 in esophageal and tongue cancer cell lines, suggesting that HPV plays a role in inducing these cancers, albeit via distinct pathways than those observed in cervical cancer. |
topic |
HPV18 Esophageal squamous cell carcinoma Tongue squamous cell carcinoma E6 E7 |
url |
https://doi.org/10.1186/s12885-019-6413-7 |
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