Bone Tissue Response to Different Grown Crystal Batches of Octacalcium Phosphate in Rat Long Bone Intramedullary Canal Area
The microstructure of biomaterials influences the cellular and biological responses in the bone. Octacalcium phosphate (OCP) exhibits higher biodegradability and osteoconductivity than hydroxyapatite (HA) during the conversion process from OCP to HA. However, the effect of the microstructure of OCP...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2021-09-01
|
Series: | International Journal of Molecular Sciences |
Subjects: | |
Online Access: | https://www.mdpi.com/1422-0067/22/18/9770 |
id |
doaj-6e366744d0404eef9074fcf3fe723539 |
---|---|
record_format |
Article |
spelling |
doaj-6e366744d0404eef9074fcf3fe7235392021-09-26T00:22:42ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-09-01229770977010.3390/ijms22189770Bone Tissue Response to Different Grown Crystal Batches of Octacalcium Phosphate in Rat Long Bone Intramedullary Canal AreaYukari Shiwaku0Ryo Hamai1Shinichi Sato2Susumu Sakai3Kaori Tsuchiya4Kazuyoshi Baba5Tetsu Takahashi6Osamu Suzuki7Division of Craniofacial Function Engineering, Tohoku University Graduate School of Dentistry, Sendai 980-8575, JapanDivision of Craniofacial Function Engineering, Tohoku University Graduate School of Dentistry, Sendai 980-8575, JapanDivision of Craniofacial Function Engineering, Tohoku University Graduate School of Dentistry, Sendai 980-8575, JapanDivision of Craniofacial Function Engineering, Tohoku University Graduate School of Dentistry, Sendai 980-8575, JapanDivision of Craniofacial Function Engineering, Tohoku University Graduate School of Dentistry, Sendai 980-8575, JapanDivision of Craniofacial Function Engineering, Tohoku University Graduate School of Dentistry, Sendai 980-8575, JapanDivision of Oral and Maxillofacial Surgery, Tohoku University Graduate School of Dentistry, Sendai 980-8575, JapanDivision of Craniofacial Function Engineering, Tohoku University Graduate School of Dentistry, Sendai 980-8575, JapanThe microstructure of biomaterials influences the cellular and biological responses in the bone. Octacalcium phosphate (OCP) exhibits higher biodegradability and osteoconductivity than hydroxyapatite (HA) during the conversion process from OCP to HA. However, the effect of the microstructure of OCP crystals on long tubular bones has not been clarified. In this study, two types of OCPs with different microstructures, fine-OCP (F-OCP) and coarse-OCP (C-OCP), were implanted in rat tibia for 4 weeks. F-OCP promoted cortical bone regeneration compared with C-OCP. The osteoclasts appearance was significantly higher in the C-OCP group than in the control group (defect only) at 1-week post-implantation. To investigate whether the solubility equilibrium depends on the different particle sizes of OCPs, Nano-OCP, which consisted of nanometer-sized OCPs, was prepared. The degree of supersaturation (DS) tended to decrease modestly in the order of C-OCP, F-OCP, and Nano-OCP with respect to HA and OCP in Tris-HCl buffer. F-OCP showed a higher phosphate ion concentration and lower calcium ion concentration after immersion in the buffer than C-OCP. The crystal structures of both OCPs tended to be converted to HA by rat abdominal implantation. These results suggest that differences in the microstructure of OCPs may affect osteoclastogenesis and result in osteoconductivity of this material in long tubular bone by altering dissolution behavior.https://www.mdpi.com/1422-0067/22/18/9770octacalcium phosphatecrystal microstructurebone formationsolubility equilibriumosteoclast-like cells |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yukari Shiwaku Ryo Hamai Shinichi Sato Susumu Sakai Kaori Tsuchiya Kazuyoshi Baba Tetsu Takahashi Osamu Suzuki |
spellingShingle |
Yukari Shiwaku Ryo Hamai Shinichi Sato Susumu Sakai Kaori Tsuchiya Kazuyoshi Baba Tetsu Takahashi Osamu Suzuki Bone Tissue Response to Different Grown Crystal Batches of Octacalcium Phosphate in Rat Long Bone Intramedullary Canal Area International Journal of Molecular Sciences octacalcium phosphate crystal microstructure bone formation solubility equilibrium osteoclast-like cells |
author_facet |
Yukari Shiwaku Ryo Hamai Shinichi Sato Susumu Sakai Kaori Tsuchiya Kazuyoshi Baba Tetsu Takahashi Osamu Suzuki |
author_sort |
Yukari Shiwaku |
title |
Bone Tissue Response to Different Grown Crystal Batches of Octacalcium Phosphate in Rat Long Bone Intramedullary Canal Area |
title_short |
Bone Tissue Response to Different Grown Crystal Batches of Octacalcium Phosphate in Rat Long Bone Intramedullary Canal Area |
title_full |
Bone Tissue Response to Different Grown Crystal Batches of Octacalcium Phosphate in Rat Long Bone Intramedullary Canal Area |
title_fullStr |
Bone Tissue Response to Different Grown Crystal Batches of Octacalcium Phosphate in Rat Long Bone Intramedullary Canal Area |
title_full_unstemmed |
Bone Tissue Response to Different Grown Crystal Batches of Octacalcium Phosphate in Rat Long Bone Intramedullary Canal Area |
title_sort |
bone tissue response to different grown crystal batches of octacalcium phosphate in rat long bone intramedullary canal area |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2021-09-01 |
description |
The microstructure of biomaterials influences the cellular and biological responses in the bone. Octacalcium phosphate (OCP) exhibits higher biodegradability and osteoconductivity than hydroxyapatite (HA) during the conversion process from OCP to HA. However, the effect of the microstructure of OCP crystals on long tubular bones has not been clarified. In this study, two types of OCPs with different microstructures, fine-OCP (F-OCP) and coarse-OCP (C-OCP), were implanted in rat tibia for 4 weeks. F-OCP promoted cortical bone regeneration compared with C-OCP. The osteoclasts appearance was significantly higher in the C-OCP group than in the control group (defect only) at 1-week post-implantation. To investigate whether the solubility equilibrium depends on the different particle sizes of OCPs, Nano-OCP, which consisted of nanometer-sized OCPs, was prepared. The degree of supersaturation (DS) tended to decrease modestly in the order of C-OCP, F-OCP, and Nano-OCP with respect to HA and OCP in Tris-HCl buffer. F-OCP showed a higher phosphate ion concentration and lower calcium ion concentration after immersion in the buffer than C-OCP. The crystal structures of both OCPs tended to be converted to HA by rat abdominal implantation. These results suggest that differences in the microstructure of OCPs may affect osteoclastogenesis and result in osteoconductivity of this material in long tubular bone by altering dissolution behavior. |
topic |
octacalcium phosphate crystal microstructure bone formation solubility equilibrium osteoclast-like cells |
url |
https://www.mdpi.com/1422-0067/22/18/9770 |
work_keys_str_mv |
AT yukarishiwaku bonetissueresponsetodifferentgrowncrystalbatchesofoctacalciumphosphateinratlongboneintramedullarycanalarea AT ryohamai bonetissueresponsetodifferentgrowncrystalbatchesofoctacalciumphosphateinratlongboneintramedullarycanalarea AT shinichisato bonetissueresponsetodifferentgrowncrystalbatchesofoctacalciumphosphateinratlongboneintramedullarycanalarea AT susumusakai bonetissueresponsetodifferentgrowncrystalbatchesofoctacalciumphosphateinratlongboneintramedullarycanalarea AT kaoritsuchiya bonetissueresponsetodifferentgrowncrystalbatchesofoctacalciumphosphateinratlongboneintramedullarycanalarea AT kazuyoshibaba bonetissueresponsetodifferentgrowncrystalbatchesofoctacalciumphosphateinratlongboneintramedullarycanalarea AT tetsutakahashi bonetissueresponsetodifferentgrowncrystalbatchesofoctacalciumphosphateinratlongboneintramedullarycanalarea AT osamusuzuki bonetissueresponsetodifferentgrowncrystalbatchesofoctacalciumphosphateinratlongboneintramedullarycanalarea |
_version_ |
1717366328806866944 |