Characterization of cry1Cb3 and cry1Fb7 from Bacillus thuringiensis subsp. galleriae
Two cry1-type genes encoding insecticidal crystal proteins (ICPs) were detected by PCR-RFLP and cloned from Bacillus thuringiensis subsp. galleriae 87. The nucleotide sequences were deposited in GenBank with accession numbers EU679501 and EU679502, and designated as cry1Fb7 and cry1Cb3 respectively...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
De Gruyter
2015-11-01
|
Series: | Open Life Sciences |
Subjects: | |
Online Access: | http://www.degruyter.com/view/j/biol.2015.10.issue-1/biol-2015-0054/biol-2015-0054.xml?format=INT |
id |
doaj-6e3f4424dfae405ca9a07194b5856354 |
---|---|
record_format |
Article |
spelling |
doaj-6e3f4424dfae405ca9a07194b58563542020-11-24T22:17:53ZengDe GruyterOpen Life Sciences2391-54122015-11-0110110.1515/biol-2015-0054biol-2015-0054Characterization of cry1Cb3 and cry1Fb7 from Bacillus thuringiensis subsp. galleriaeHuang Tianpei0Xiao Ying1Pan Jieru2Zhang Lingling3Gelbič Ivan4Guan Xiong5Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education & Fujian- Taiwan Joint Center for Ecological Control of Crop Pests, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, People’s Republic of ChinaKey Laboratory of Biopesticide and Chemical Biology, Ministry of Education & Fujian- Taiwan Joint Center for Ecological Control of Crop Pests, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, People’s Republic of ChinaFuzhou Center for Disease Control and Prevention, Fuzhou, Fujian 350004, People’s Republic of ChinaKey Laboratory of Biopesticide and Chemical Biology, Ministry of Education & Fujian- Taiwan Joint Center for Ecological Control of Crop Pests, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, People’s Republic of ChinaBiology Centre of the Academy of Sciences of the Czech Republic, Institute of Entomology, Branišovská 31, 370 05 České Budějovice, Czech RepublicKey Laboratory of Biopesticide and Chemical Biology, Ministry of Education & Fujian- Taiwan Joint Center for Ecological Control of Crop Pests, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, People’s Republic of ChinaTwo cry1-type genes encoding insecticidal crystal proteins (ICPs) were detected by PCR-RFLP and cloned from Bacillus thuringiensis subsp. galleriae 87. The nucleotide sequences were deposited in GenBank with accession numbers EU679501 and EU679502, and designated as cry1Fb7 and cry1Cb3 respectively by B. thuringiensis Delta- Endotoxin Nomenclature Committee. cry1Cb3 shared 99% homology with other cry1Cb genes. The existence of two additional stop codons indicated cry1Cb3 was a silent gene. The cry1Cb3 was 3531 bp with 38.98% G+C content and its first open reading frame (ORF) encoded a protein of 213 amino acid residues with a calculated molecular weight of 23.8 kDa and a predicted pI value of 4.63. Five amino acid sequence blocks (block 1, block 2, block 3, block 4 and block 5) were found in Cry1Cb3. Translation of cry1Fb7 revealed an ORF of 3525 bp with 39.12% G+C content and a protein with a calculated molecular weight of 133.2 kDa and a predicted pI value of 5.18. Cry1Fb7 had five amino acid sequence blocks (blocks 1, 2, 3, 4 and 5) and three domains (I, II and III), which consisted of 218 residues (Leu34 to Ala252), 197 residues (Thr257 to Asp454), and 138 residues (Ile464 to Glu600), respectively.http://www.degruyter.com/view/j/biol.2015.10.issue-1/biol-2015-0054/biol-2015-0054.xml?format=INTBacillus thuringiensis subsp. galleriaePCRRFLP cloning cry1Cb cry1Fb |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Huang Tianpei Xiao Ying Pan Jieru Zhang Lingling Gelbič Ivan Guan Xiong |
spellingShingle |
Huang Tianpei Xiao Ying Pan Jieru Zhang Lingling Gelbič Ivan Guan Xiong Characterization of cry1Cb3 and cry1Fb7 from Bacillus thuringiensis subsp. galleriae Open Life Sciences Bacillus thuringiensis subsp. galleriae PCRRFLP cloning cry1Cb cry1Fb |
author_facet |
Huang Tianpei Xiao Ying Pan Jieru Zhang Lingling Gelbič Ivan Guan Xiong |
author_sort |
Huang Tianpei |
title |
Characterization of cry1Cb3 and cry1Fb7 from
Bacillus thuringiensis subsp. galleriae |
title_short |
Characterization of cry1Cb3 and cry1Fb7 from
Bacillus thuringiensis subsp. galleriae |
title_full |
Characterization of cry1Cb3 and cry1Fb7 from
Bacillus thuringiensis subsp. galleriae |
title_fullStr |
Characterization of cry1Cb3 and cry1Fb7 from
Bacillus thuringiensis subsp. galleriae |
title_full_unstemmed |
Characterization of cry1Cb3 and cry1Fb7 from
Bacillus thuringiensis subsp. galleriae |
title_sort |
characterization of cry1cb3 and cry1fb7 from
bacillus thuringiensis subsp. galleriae |
publisher |
De Gruyter |
series |
Open Life Sciences |
issn |
2391-5412 |
publishDate |
2015-11-01 |
description |
Two cry1-type genes encoding insecticidal crystal
proteins (ICPs) were detected by PCR-RFLP and cloned from
Bacillus thuringiensis subsp. galleriae 87. The nucleotide
sequences were deposited in GenBank with accession
numbers EU679501 and EU679502, and designated as
cry1Fb7 and cry1Cb3 respectively by B. thuringiensis Delta-
Endotoxin Nomenclature Committee. cry1Cb3 shared 99%
homology with other cry1Cb genes. The existence of two
additional stop codons indicated cry1Cb3 was a silent gene.
The cry1Cb3 was 3531 bp with 38.98% G+C content and its
first open reading frame (ORF) encoded a protein of 213
amino acid residues with a calculated molecular weight
of 23.8 kDa and a predicted pI value of 4.63. Five amino
acid sequence blocks (block 1, block 2, block 3, block 4
and block 5) were found in Cry1Cb3. Translation of cry1Fb7
revealed an ORF of 3525 bp with 39.12% G+C content and a
protein with a calculated molecular weight of 133.2 kDa and
a predicted pI value of 5.18. Cry1Fb7 had five amino acid
sequence blocks (blocks 1, 2, 3, 4 and 5) and three domains
(I, II and III), which consisted of 218 residues (Leu34 to
Ala252), 197 residues (Thr257 to Asp454), and 138 residues (Ile464
to Glu600), respectively. |
topic |
Bacillus thuringiensis subsp. galleriae PCRRFLP cloning cry1Cb cry1Fb |
url |
http://www.degruyter.com/view/j/biol.2015.10.issue-1/biol-2015-0054/biol-2015-0054.xml?format=INT |
work_keys_str_mv |
AT huangtianpei characterizationofcry1cb3andcry1fb7frombacillusthuringiensissubspgalleriae AT xiaoying characterizationofcry1cb3andcry1fb7frombacillusthuringiensissubspgalleriae AT panjieru characterizationofcry1cb3andcry1fb7frombacillusthuringiensissubspgalleriae AT zhanglingling characterizationofcry1cb3andcry1fb7frombacillusthuringiensissubspgalleriae AT gelbicivan characterizationofcry1cb3andcry1fb7frombacillusthuringiensissubspgalleriae AT guanxiong characterizationofcry1cb3andcry1fb7frombacillusthuringiensissubspgalleriae |
_version_ |
1725783996346925056 |