Effect of Substrate and Culture Conditions on the Production of Amylase and Pullulanase by Thermophilic Clostridium thermosulforegenes SVM17 in Solid State Fermentation
The endo acting enzyme with dual specificity towards α-1,4- and α-1,6-glycosidic linkages are named as amylopullulanase. The production of extracellular thermostable amylopullulanase by Clostridium thermosulfurogenes SVM17 was investigated in solid state fermentation (SSF). Coarse type wheat bran w...
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Malaysian Society for Microbiology
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Online Access: | http://web.usm.my/mjm/issues/vol7no1/research4.pdf |
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doaj-6e516f8014d1458195874b99643349412020-11-24T23:51:08ZengMalaysian Society for MicrobiologyMalaysian Journal of Microbiology1823-82622231-75382011-01-01711925Effect of Substrate and Culture Conditions on the Production of Amylase and Pullulanase by Thermophilic Clostridium thermosulforegenes SVM17 in Solid State FermentationSeenayya, G.Reddy, G.Mrudula, S.The endo acting enzyme with dual specificity towards α-1,4- and α-1,6-glycosidic linkages are named as amylopullulanase. The production of extracellular thermostable amylopullulanase by Clostridium thermosulfurogenes SVM17 was investigated in solid state fermentation (SSF). Coarse type wheat bran was found to be the best substrate among ten easily available complex organic substrates evaluated. The production of enzyme reached a peak in 72 h. A high level of enzyme was produced in wheat bran moistened with PYE medium with a moisture content of 73 %. The optimum temperature and pH for amylopullulanase production was 60 °C and 7.5, respectively. An inoculum size of 20 % resulted in maximum production of amylopullulanase. Under the optimum conditions the strain showed a maximum of 17,227 and 21,526 U of amylase and pullulanase activity, respectively per kilogram of bacterial bran (BB). The enzyme production was high in SSF than that in SmF. The use of SSF for the production of thermostable amylopullulanase by C. thermosulfurogenes SVM17 could, therefore led to reduction in the overall cost of enzyme production. http://web.usm.my/mjm/issues/vol7no1/research4.pdfClostridium thermosulfurogenesAmylase activityPullulanase activityProcess parametersSolid state fermentation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Seenayya, G. Reddy, G. Mrudula, S. |
spellingShingle |
Seenayya, G. Reddy, G. Mrudula, S. Effect of Substrate and Culture Conditions on the Production of Amylase and Pullulanase by Thermophilic Clostridium thermosulforegenes SVM17 in Solid State Fermentation Malaysian Journal of Microbiology Clostridium thermosulfurogenes Amylase activity Pullulanase activity Process parameters Solid state fermentation |
author_facet |
Seenayya, G. Reddy, G. Mrudula, S. |
author_sort |
Seenayya, G. |
title |
Effect of Substrate and Culture Conditions on the Production of Amylase and Pullulanase by Thermophilic Clostridium thermosulforegenes SVM17 in Solid State Fermentation |
title_short |
Effect of Substrate and Culture Conditions on the Production of Amylase and Pullulanase by Thermophilic Clostridium thermosulforegenes SVM17 in Solid State Fermentation |
title_full |
Effect of Substrate and Culture Conditions on the Production of Amylase and Pullulanase by Thermophilic Clostridium thermosulforegenes SVM17 in Solid State Fermentation |
title_fullStr |
Effect of Substrate and Culture Conditions on the Production of Amylase and Pullulanase by Thermophilic Clostridium thermosulforegenes SVM17 in Solid State Fermentation |
title_full_unstemmed |
Effect of Substrate and Culture Conditions on the Production of Amylase and Pullulanase by Thermophilic Clostridium thermosulforegenes SVM17 in Solid State Fermentation |
title_sort |
effect of substrate and culture conditions on the production of amylase and pullulanase by thermophilic clostridium thermosulforegenes svm17 in solid state fermentation |
publisher |
Malaysian Society for Microbiology |
series |
Malaysian Journal of Microbiology |
issn |
1823-8262 2231-7538 |
publishDate |
2011-01-01 |
description |
The endo acting enzyme with dual specificity towards α-1,4- and α-1,6-glycosidic linkages are named as amylopullulanase. The production of extracellular thermostable amylopullulanase by Clostridium thermosulfurogenes SVM17 was investigated in solid state fermentation (SSF). Coarse type wheat bran was found to be the best substrate among ten easily available complex organic substrates evaluated. The production of enzyme reached a peak in 72 h. A high level of enzyme was produced in wheat bran moistened with PYE medium with a moisture content of 73 %. The optimum temperature and pH for amylopullulanase production was 60 °C and 7.5, respectively. An inoculum size of 20 % resulted in maximum production of amylopullulanase. Under the optimum conditions the strain showed a maximum of 17,227 and 21,526 U of amylase and pullulanase activity, respectively per kilogram of bacterial bran (BB). The enzyme production was high in SSF than that in SmF. The use of SSF for the production of thermostable amylopullulanase by C. thermosulfurogenes SVM17 could, therefore led to reduction in the overall cost of enzyme production. |
topic |
Clostridium thermosulfurogenes Amylase activity Pullulanase activity Process parameters Solid state fermentation |
url |
http://web.usm.my/mjm/issues/vol7no1/research4.pdf |
work_keys_str_mv |
AT seenayyag effectofsubstrateandcultureconditionsontheproductionofamylaseandpullulanasebythermophilicclostridiumthermosulforegenessvm17insolidstatefermentation AT reddyg effectofsubstrateandcultureconditionsontheproductionofamylaseandpullulanasebythermophilicclostridiumthermosulforegenessvm17insolidstatefermentation AT mrudulas effectofsubstrateandcultureconditionsontheproductionofamylaseandpullulanasebythermophilicclostridiumthermosulforegenessvm17insolidstatefermentation |
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1725477271613997056 |