Molecular identification of Trichuris trichiura and Hymenolepis diminuta in long-tailed macaques (Macaca fascicularis) in Lopburi, Thailand
Background and Aim: Trichuris trichiura and Hymenolepis diminuta are helminthic intestinal parasites that infect humans and other animals, including non-human primates. However, molecular detection of these parasites remains scarce in long-tailed macaques (Macaca fascicularis), which coexist with hu...
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doaj-6e626eb57433457a83ab933c788892722021-08-02T20:48:37ZengVeterinary WorldVeterinary World0972-89882231-09162021-04-0114488488810.14202/vetworld.2021.884-888Molecular identification of Trichuris trichiura and Hymenolepis diminuta in long-tailed macaques (Macaca fascicularis) in Lopburi, ThailandWanat Sricharern0https://orcid.org/0000-0001-9552-2335Tawin Inpankaew1https://orcid.org/0000-0001-5982-4811Sarawan Kaewmongkol2https://orcid.org/0000-0002-6203-1633Thitichai Jarudecha3https://orcid.org/0000-0001-5052-2358Natnaree Inthong4https://orcid.org/0000-0002-3853-8899Center for Agricultural Biotechnology, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom, Thailand; Center of Excellence on Agricultural Biotechnology, Science and Technology Postgraduate Education and Research Development Office, Commission on Higher Education, Ministry of Education, Science, Research Innovation (AG-BIO/PERDO-CHE), Bangkok Thailand; Department of Veterinary Technology, Faculty of Veterinary Technology, Kasetsart University, Bangkok, Thailand.Center for Agricultural Biotechnology, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom, Thailand; 2. Center of Excellence on Agricultural Biotechnology, Science and Technology Postgraduate Education and Research Development Office, Commission on Higher Education, Ministry of Education, Science, Research Innovation (AG-BIO/PERDO-CHE), Bangkok Thailand; Department of Parasitology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand.Department of Veterinary Technology, Faculty of Veterinary Technology, Kasetsart University, Bangkok, Thailand.Department of Veterinary Technology, Faculty of Veterinary Technology, Kasetsart University, Bangkok, Thailand.Department of Veterinary Technology, Faculty of Veterinary Technology, Kasetsart University, Bangkok, Thailand.Background and Aim: Trichuris trichiura and Hymenolepis diminuta are helminthic intestinal parasites that infect humans and other animals, including non-human primates. However, molecular detection of these parasites remains scarce in long-tailed macaques (Macaca fascicularis), which coexist with human communities in Thailand. Thus, this study aimed to molecularly confirm the occurrence of Trichuris spp. and Hymenolepis spp. infection and determine the species of both parasites that were found in long-tailed macaques. Materials and Methods: A total of 200 fecal samples were randomly collected from long-tailed macaques living in Lopburi, Thailand, and tested based on polymerase chain reaction (PCR) assays for Trichuris spp. and Hymenolepis spp. infections. The PCR products were submitted for DNA purification and sequencing. Phylogenetic analysis was performed using the maximum likelihood method. Results: Of 200 tested samples, three (1.5%) were positive for Trichuris spp. Sequence analysis of all positive samples revealed the presence of T. trichiura, while eight samples (8/200, 4%) positive for Hymenolepis spp. were classified as H. diminuta. No significant associations were found between parasite infection and sex of macaques. Conclusion: This study revealed that long-tailed macaques harbor T. trichiura and H. diminuta. These results suggested that local residents and tourists must pay attention to limiting contact with long-tailed macaques and take hygienic precautions to reduce the risk of zoonotic and anthroponotic transmission of these parasites between humans and long-tailed macaques.http://www.veterinaryworld.org/Vol.14/April-2021/11.pdfhymenolepis diminutalong-tailed macaquemacaca fascicularisthailandtrichuris trichiura |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wanat Sricharern Tawin Inpankaew Sarawan Kaewmongkol Thitichai Jarudecha Natnaree Inthong |
spellingShingle |
Wanat Sricharern Tawin Inpankaew Sarawan Kaewmongkol Thitichai Jarudecha Natnaree Inthong Molecular identification of Trichuris trichiura and Hymenolepis diminuta in long-tailed macaques (Macaca fascicularis) in Lopburi, Thailand Veterinary World hymenolepis diminuta long-tailed macaque macaca fascicularis thailand trichuris trichiura |
author_facet |
Wanat Sricharern Tawin Inpankaew Sarawan Kaewmongkol Thitichai Jarudecha Natnaree Inthong |
author_sort |
Wanat Sricharern |
title |
Molecular identification of Trichuris trichiura and Hymenolepis diminuta in long-tailed macaques (Macaca fascicularis) in Lopburi, Thailand |
title_short |
Molecular identification of Trichuris trichiura and Hymenolepis diminuta in long-tailed macaques (Macaca fascicularis) in Lopburi, Thailand |
title_full |
Molecular identification of Trichuris trichiura and Hymenolepis diminuta in long-tailed macaques (Macaca fascicularis) in Lopburi, Thailand |
title_fullStr |
Molecular identification of Trichuris trichiura and Hymenolepis diminuta in long-tailed macaques (Macaca fascicularis) in Lopburi, Thailand |
title_full_unstemmed |
Molecular identification of Trichuris trichiura and Hymenolepis diminuta in long-tailed macaques (Macaca fascicularis) in Lopburi, Thailand |
title_sort |
molecular identification of trichuris trichiura and hymenolepis diminuta in long-tailed macaques (macaca fascicularis) in lopburi, thailand |
publisher |
Veterinary World |
series |
Veterinary World |
issn |
0972-8988 2231-0916 |
publishDate |
2021-04-01 |
description |
Background and Aim: Trichuris trichiura and Hymenolepis diminuta are helminthic intestinal parasites that infect humans and other animals, including non-human primates. However, molecular detection of these parasites remains scarce in long-tailed macaques (Macaca fascicularis), which coexist with human communities in Thailand. Thus, this study aimed to molecularly confirm the occurrence of Trichuris spp. and Hymenolepis spp. infection and determine the species of both parasites that were found in long-tailed macaques.
Materials and Methods: A total of 200 fecal samples were randomly collected from long-tailed macaques living in Lopburi, Thailand, and tested based on polymerase chain reaction (PCR) assays for Trichuris spp. and Hymenolepis spp. infections. The PCR products were submitted for DNA purification and sequencing. Phylogenetic analysis was performed using the maximum likelihood method.
Results: Of 200 tested samples, three (1.5%) were positive for Trichuris spp. Sequence analysis of all positive samples revealed the presence of T. trichiura, while eight samples (8/200, 4%) positive for Hymenolepis spp. were classified as H. diminuta. No significant associations were found between parasite infection and sex of macaques.
Conclusion: This study revealed that long-tailed macaques harbor T. trichiura and H. diminuta. These results suggested that local residents and tourists must pay attention to limiting contact with long-tailed macaques and take hygienic precautions to reduce the risk of zoonotic and anthroponotic transmission of these parasites between humans and long-tailed macaques. |
topic |
hymenolepis diminuta long-tailed macaque macaca fascicularis thailand trichuris trichiura |
url |
http://www.veterinaryworld.org/Vol.14/April-2021/11.pdf |
work_keys_str_mv |
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