Study of Biofilm Formation Among Clinical Staphylococcal Isolates
Introduction: Biofilm forming microorganisms are related to chronic and recurrent human infections as well as are highly resistant to antimicrobial agents. Various methods have been used in medical field for the detection of biofilm production which includes visual assessment by electron microsc...
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doaj-6e69a3303e124df6be69a2edf0f851bb2020-11-25T02:13:23ZengJCDR Research and Publications Pvt. Ltd.National Journal of Laboratory Medicine2277-85512455-68822015-10-0144242710.7860/NJLM/2015/13975:2062Study of Biofilm Formation Among Clinical Staphylococcal Isolates Saumya Singh 0 Ashish K Asthana1Molly Madan2S. J. Ghosh3Assistant Professor, Department of Microbiology, Subharti Medical College, Swami Vivekanand Subharti University, Meerut, Uttar Pradesh, India.Assistant Professor, Department of Microbiology, Subharti Medical College, Swami Vivekanand Subharti University, Meerut, Uttar Pradesh, India.Professor, Department of Microbiology, Subharti Medical College, Swami Vivekanand Subharti University, Meerut, Uttar Pradesh, India.Professor, Department of Microbiology. D. Y. Patil Medical College. D. Y. Patil University, Kolhapur, India.Introduction: Biofilm forming microorganisms are related to chronic and recurrent human infections as well as are highly resistant to antimicrobial agents. Various methods have been used in medical field for the detection of biofilm production which includes visual assessment by electron microscopy and polymerase chain reaction, quantitative method like Tissue Culture Plate (TCP) method and two qualitative methods such as Tube Method (TM) and Congo Red Agar (CRA) method. Objectives: To detect the prevalence of biofilm formation in Staphylococci, to evaluate two different methods i.e. TM and CRA for the detection of biofilms and to see its relation with antimicrobial resistance. Materials and Method: The study was conducted at Department of Microbiology, Dr. D.Y. Patil Hospital and Research Centre during the period June 2011 to June 2013. A total of 130 clinical isolates were subjected to biofilm detection methods. Standard microbiological procedure was done to identify the isolates. Biofilm detection was tested by TM and CRA. Kirby-Bauer disc diffusion technique was performed to do antibiotic susceptibility test of biofilm producing bacteria according to CLSI guidelines. Results: From the total of 130 clinical isolates, biofilm productions among Staphylococci were 59.23%. And the percentage of biofilm detection of Staphylococci isolates by Congo red method were 31.54% and by test tube method 50%. Hence, the TM method was considered to be superior to CRA. We have also observed higher antibiotic resistance in biofilm producing bacteria than non-biofilm producers. Conclusion: We can conclude from our study that the TM method is a more suitable and reliable method as compared to CRA and can be recommended as a general screening method in laboratories for detecting biofilm forming bacteria.http://www.njlm.net/articles/PDF/2062/6-%2013975_F(AK)_PF1(VSUAK)_PFA(AK)_PF2(PVSU).pdfantimicrobial resistancecongo red agar methodtest tube method |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Saumya Singh Ashish K Asthana Molly Madan S. J. Ghosh |
spellingShingle |
Saumya Singh Ashish K Asthana Molly Madan S. J. Ghosh Study of Biofilm Formation Among Clinical Staphylococcal Isolates National Journal of Laboratory Medicine antimicrobial resistance congo red agar method test tube method |
author_facet |
Saumya Singh Ashish K Asthana Molly Madan S. J. Ghosh |
author_sort |
Saumya Singh |
title |
Study of Biofilm Formation Among Clinical Staphylococcal Isolates |
title_short |
Study of Biofilm Formation Among Clinical Staphylococcal Isolates |
title_full |
Study of Biofilm Formation Among Clinical Staphylococcal Isolates |
title_fullStr |
Study of Biofilm Formation Among Clinical Staphylococcal Isolates |
title_full_unstemmed |
Study of Biofilm Formation Among Clinical Staphylococcal Isolates |
title_sort |
study of biofilm formation among clinical staphylococcal isolates |
publisher |
JCDR Research and Publications Pvt. Ltd. |
series |
National Journal of Laboratory Medicine |
issn |
2277-8551 2455-6882 |
publishDate |
2015-10-01 |
description |
Introduction: Biofilm forming microorganisms are related to
chronic and recurrent human infections as well as are highly
resistant to antimicrobial agents. Various methods have been
used in medical field for the detection of biofilm production
which includes visual assessment by electron microscopy and
polymerase chain reaction, quantitative method like Tissue
Culture Plate (TCP) method and two qualitative methods such
as Tube Method (TM) and Congo Red Agar (CRA) method.
Objectives: To detect the prevalence of biofilm formation in
Staphylococci, to evaluate two different methods i.e. TM and
CRA for the detection of biofilms and to see its relation with
antimicrobial resistance.
Materials and Method: The study was conducted at
Department of Microbiology, Dr. D.Y. Patil Hospital and
Research Centre during the period June 2011 to June 2013.
A total of 130 clinical isolates were subjected to biofilm detection
methods. Standard microbiological procedure was done to
identify the isolates. Biofilm detection was tested by TM and
CRA. Kirby-Bauer disc diffusion technique was performed to
do antibiotic susceptibility test of biofilm producing bacteria
according to CLSI guidelines.
Results: From the total of 130 clinical isolates, biofilm
productions among Staphylococci were 59.23%. And the
percentage of biofilm detection of Staphylococci isolates by
Congo red method were 31.54% and by test tube method
50%. Hence, the TM method was considered to be superior
to CRA. We have also observed higher antibiotic resistance in
biofilm producing bacteria than non-biofilm producers.
Conclusion: We can conclude from our study that the TM
method is a more suitable and reliable method as compared
to CRA and can be recommended as a general screening
method in laboratories for detecting biofilm forming bacteria. |
topic |
antimicrobial resistance congo red agar method test tube method |
url |
http://www.njlm.net/articles/PDF/2062/6-%2013975_F(AK)_PF1(VSUAK)_PFA(AK)_PF2(PVSU).pdf |
work_keys_str_mv |
AT saumyasingh studyofbiofilmformationamongclinicalstaphylococcalisolates AT ashishkasthana studyofbiofilmformationamongclinicalstaphylococcalisolates AT mollymadan studyofbiofilmformationamongclinicalstaphylococcalisolates AT sjghosh studyofbiofilmformationamongclinicalstaphylococcalisolates |
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