Exploring the Dual Interaction of Natural Rhamnolipids with Plant and Fungal Biomimetic Plasma Membranes through Biophysical Studies
Rhamnolipids (RLs) are potential biocontrol agents for crop culture protection. Their mode of action has been proposed as dual, combining plant protection activation and antifungal activities. The present work focuses on the interaction of natural RLs with plant and fungi membrane models at the mole...
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doaj-6e6c1af935e046888d16f9c1cc34dc652020-11-25T00:35:38ZengMDPI AGInternational Journal of Molecular Sciences1422-00672019-02-01205100910.3390/ijms20051009ijms20051009Exploring the Dual Interaction of Natural Rhamnolipids with Plant and Fungal Biomimetic Plasma Membranes through Biophysical StudiesNoadya Monnier0Aurélien L. Furlan1Sébastien Buchoux2Magali Deleu3Manuel Dauchez4Sonia Rippa5Catherine Sarazin6Unité de Génie Enzymatique et Cellulaire, CNRS UMR 7025, Université de Picardie Jules Verne (UPJV), 80039 Amiens, FranceUnité de Génie Enzymatique et Cellulaire, CNRS UMR 7025, Université de Picardie Jules Verne (UPJV), 80039 Amiens, FranceUnité de Génie Enzymatique et Cellulaire, CNRS UMR 7025, Université de Picardie Jules Verne (UPJV), 80039 Amiens, FranceLaboratoire de Biophysique Moléculaire aux Interfaces, Gembloux Agro-Bio Tech, B5030 Gembloux, BelgiumMatrice Extracellulaire et Dynamique Cellulaire, UMR CNRS 7369, Chaire MAgICS, Université de Reims Champagne-Ardenne (URCA), 51687 Reims, FranceUnité de Génie Enzymatique et Cellulaire, CNRS UMR 7025, Sorbonne Universités, Université de Technologie de Compiègne, 60200 Compiègne, FranceUnité de Génie Enzymatique et Cellulaire, CNRS UMR 7025, Université de Picardie Jules Verne (UPJV), 80039 Amiens, FranceRhamnolipids (RLs) are potential biocontrol agents for crop culture protection. Their mode of action has been proposed as dual, combining plant protection activation and antifungal activities. The present work focuses on the interaction of natural RLs with plant and fungi membrane models at the molecular scale. Representative models were constructed and the interaction with RLs was studied by Fourier transform infrared (FTIR) and deuterium nuclear magnetic resonance (<sup>2</sup>H NMR) spectroscopic measurements. Molecular dynamic (MD) simulations were performed to investigate RL insertion in lipid bilayers. Our results showed that the RLs fit into the membrane models and were located near the lipid phosphate group of the phospholipid bilayers, nearby phospholipid glycerol backbones. The results obtained with plant plasma membrane models suggest that the insertion of RLs inside the lipid bilayer did not significantly affect lipid dynamics. Oppositely, a clear fluidity increase of fungi membrane models was observed. This effect was related to the presence and the specific structure of ergosterol. The nature of the phytosterols could also influence the RL effect on plant plasma membrane destabilization. Subtle changes in lipid dynamics could then be linked with plant defense induction and the more drastic effects associated with fungal membrane destabilization.https://www.mdpi.com/1422-0067/20/5/1009rhamnolipidmembrane insertion and dynamicbiomimetic lipid membraneplant plasma membranefungi plasma membranemembrane sterolphytosterolsergosterol |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Noadya Monnier Aurélien L. Furlan Sébastien Buchoux Magali Deleu Manuel Dauchez Sonia Rippa Catherine Sarazin |
spellingShingle |
Noadya Monnier Aurélien L. Furlan Sébastien Buchoux Magali Deleu Manuel Dauchez Sonia Rippa Catherine Sarazin Exploring the Dual Interaction of Natural Rhamnolipids with Plant and Fungal Biomimetic Plasma Membranes through Biophysical Studies International Journal of Molecular Sciences rhamnolipid membrane insertion and dynamic biomimetic lipid membrane plant plasma membrane fungi plasma membrane membrane sterol phytosterols ergosterol |
author_facet |
Noadya Monnier Aurélien L. Furlan Sébastien Buchoux Magali Deleu Manuel Dauchez Sonia Rippa Catherine Sarazin |
author_sort |
Noadya Monnier |
title |
Exploring the Dual Interaction of Natural Rhamnolipids with Plant and Fungal Biomimetic Plasma Membranes through Biophysical Studies |
title_short |
Exploring the Dual Interaction of Natural Rhamnolipids with Plant and Fungal Biomimetic Plasma Membranes through Biophysical Studies |
title_full |
Exploring the Dual Interaction of Natural Rhamnolipids with Plant and Fungal Biomimetic Plasma Membranes through Biophysical Studies |
title_fullStr |
Exploring the Dual Interaction of Natural Rhamnolipids with Plant and Fungal Biomimetic Plasma Membranes through Biophysical Studies |
title_full_unstemmed |
Exploring the Dual Interaction of Natural Rhamnolipids with Plant and Fungal Biomimetic Plasma Membranes through Biophysical Studies |
title_sort |
exploring the dual interaction of natural rhamnolipids with plant and fungal biomimetic plasma membranes through biophysical studies |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2019-02-01 |
description |
Rhamnolipids (RLs) are potential biocontrol agents for crop culture protection. Their mode of action has been proposed as dual, combining plant protection activation and antifungal activities. The present work focuses on the interaction of natural RLs with plant and fungi membrane models at the molecular scale. Representative models were constructed and the interaction with RLs was studied by Fourier transform infrared (FTIR) and deuterium nuclear magnetic resonance (<sup>2</sup>H NMR) spectroscopic measurements. Molecular dynamic (MD) simulations were performed to investigate RL insertion in lipid bilayers. Our results showed that the RLs fit into the membrane models and were located near the lipid phosphate group of the phospholipid bilayers, nearby phospholipid glycerol backbones. The results obtained with plant plasma membrane models suggest that the insertion of RLs inside the lipid bilayer did not significantly affect lipid dynamics. Oppositely, a clear fluidity increase of fungi membrane models was observed. This effect was related to the presence and the specific structure of ergosterol. The nature of the phytosterols could also influence the RL effect on plant plasma membrane destabilization. Subtle changes in lipid dynamics could then be linked with plant defense induction and the more drastic effects associated with fungal membrane destabilization. |
topic |
rhamnolipid membrane insertion and dynamic biomimetic lipid membrane plant plasma membrane fungi plasma membrane membrane sterol phytosterols ergosterol |
url |
https://www.mdpi.com/1422-0067/20/5/1009 |
work_keys_str_mv |
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