Four methods of preparing mRNA 5' end libraries using the Illumina sequencing platform.

BACKGROUND: The 5' untranslated regions of mRNA play an important role in their translation. RESULTS: Here, we describe the development of four methods of profiling mRNA 5' ends using the Illumina sequencing platform; the first method utilizes SMART (Switching Mechanism At 5' end of R...

Full description

Bibliographic Details
Main Authors: Ryuji J Machida, Ya-Ying Lin
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4086933?pdf=render