The earthworm Dendrobaena veneta (Annelida): A new experimental-organism for photobiomodulation and wound healing
Photobiomodulation (PBM) is a manipulation of cellular behavior using non-ablative low intensity light sources. This manipulation triggers a cascade of metabolic effects and physiological changes resulting in improved tissue repair, of benefit in the treatment of tissue injury, degenerative or autoi...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
PAGEPress Publications
2018-02-01
|
Series: | European Journal of Histochemistry |
Subjects: | |
Online Access: | http://www.ejh.it/index.php/ejh/article/view/2867 |
id |
doaj-6e9fff39d32e4c2bb1999d10171699b9 |
---|---|
record_format |
Article |
spelling |
doaj-6e9fff39d32e4c2bb1999d10171699b92020-11-25T02:44:06ZengPAGEPress PublicationsEuropean Journal of Histochemistry 1121-760X2038-83062018-02-0162110.4081/ejh.2018.28671599The earthworm Dendrobaena veneta (Annelida): A new experimental-organism for photobiomodulation and wound healingAndrea Amaroli0Sara Ferrando1Marina Pozzolini2Lorenzo Gallus3Steven Parker4Stefano Benedicenti5University of Genoa, Department of Surgical Sciences and Integrated DiagnosticUniversity of Genoa, Department of Earth, Environmental and Life SciencesUniversity of Genoa, Department of Earth, Environmental and Life SciencesUniversity of Genoa, Department of Earth, Environmental and Life SciencesUniversity of Genoa, Department of Surgical Sciences and Integrated DiagnosticUniversity of Genoa, Department of Surgical Sciences and Integrated DiagnosticPhotobiomodulation (PBM) is a manipulation of cellular behavior using non-ablative low intensity light sources. This manipulation triggers a cascade of metabolic effects and physiological changes resulting in improved tissue repair, of benefit in the treatment of tissue injury, degenerative or autoimmune diseases. PBM has witnessed an exponential increase in both clinical instrument technology and applications. It is therefore of benefit to find reliable experimental models to test the burgeoning laser technology for medical applications. In our work, we proposed the earthworm Dendrobaena veneta for the study of non-ablative laser-light effects on wound healing. In our preliminary work, D. veneta has been shown to be positively affected by PBM. New tests using D. veneta were set up to evaluate the effectiveness of a chosen 808 nm-64 J/cm2–1W-CW laser therapy using the AB2799 hand-piece with flat-top bean profile, on the wound healing process of the earthworm. Effective outcome was assimilated through examining the macroscopic, histological, and molecular changes on the irradiated posterior-segment of excised-earthworms with respect to controls. Three successive treatments, one every 24 hours, were concluded as sufficient to promote the wound healing, by effects on muscular and blood vessel contraction, decrement of bacteria load, reduction of inflammatory processes and tissue degeneration. D. veneta was demonstrated to be a reliable experimental organism that meets well the 3Rs principles and the National Science Foundation statement. Through their genetic and evolutionary peculiarity, comparable to those of scientifically accredited models, D. veneta allows the effect of laser therapies by multidisciplinary methods, at various degree of complexity and costs to be investigated.http://www.ejh.it/index.php/ejh/article/view/2867Low Level Laser Therapylaser irradiationscarringanti-inflammatorytissue repairHeat Shock Proteincell proliferation. |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Andrea Amaroli Sara Ferrando Marina Pozzolini Lorenzo Gallus Steven Parker Stefano Benedicenti |
spellingShingle |
Andrea Amaroli Sara Ferrando Marina Pozzolini Lorenzo Gallus Steven Parker Stefano Benedicenti The earthworm Dendrobaena veneta (Annelida): A new experimental-organism for photobiomodulation and wound healing European Journal of Histochemistry Low Level Laser Therapy laser irradiation scarring anti-inflammatory tissue repair Heat Shock Protein cell proliferation. |
author_facet |
Andrea Amaroli Sara Ferrando Marina Pozzolini Lorenzo Gallus Steven Parker Stefano Benedicenti |
author_sort |
Andrea Amaroli |
title |
The earthworm Dendrobaena veneta (Annelida): A new experimental-organism for photobiomodulation and wound healing |
title_short |
The earthworm Dendrobaena veneta (Annelida): A new experimental-organism for photobiomodulation and wound healing |
title_full |
The earthworm Dendrobaena veneta (Annelida): A new experimental-organism for photobiomodulation and wound healing |
title_fullStr |
The earthworm Dendrobaena veneta (Annelida): A new experimental-organism for photobiomodulation and wound healing |
title_full_unstemmed |
The earthworm Dendrobaena veneta (Annelida): A new experimental-organism for photobiomodulation and wound healing |
title_sort |
earthworm dendrobaena veneta (annelida): a new experimental-organism for photobiomodulation and wound healing |
publisher |
PAGEPress Publications |
series |
European Journal of Histochemistry |
issn |
1121-760X 2038-8306 |
publishDate |
2018-02-01 |
description |
Photobiomodulation (PBM) is a manipulation of cellular behavior using non-ablative low intensity light sources. This manipulation triggers a cascade of metabolic effects and physiological changes resulting in improved tissue repair, of benefit in the treatment of tissue injury, degenerative or autoimmune diseases. PBM has witnessed an exponential increase in both clinical instrument technology and applications. It is therefore of benefit to find reliable experimental models to test the burgeoning laser technology for medical applications. In our work, we proposed the earthworm Dendrobaena veneta for the study of non-ablative laser-light effects on wound healing. In our preliminary work, D. veneta has been shown to be positively affected by PBM. New tests using D. veneta were set up to evaluate the effectiveness of a chosen 808 nm-64 J/cm2–1W-CW laser therapy using the AB2799 hand-piece with flat-top bean profile, on the wound healing process of the earthworm. Effective outcome was assimilated through examining the macroscopic, histological, and molecular changes on the irradiated posterior-segment of excised-earthworms with respect to controls. Three successive treatments, one every 24 hours, were concluded as sufficient to promote the wound healing, by effects on muscular and blood vessel contraction, decrement of bacteria load, reduction of inflammatory processes and tissue degeneration. D. veneta was demonstrated to be a reliable experimental organism that meets well the 3Rs principles and the National Science Foundation statement. Through their genetic and evolutionary peculiarity, comparable to those of scientifically accredited models, D. veneta allows the effect of laser therapies by multidisciplinary methods, at various degree of complexity and costs to be investigated. |
topic |
Low Level Laser Therapy laser irradiation scarring anti-inflammatory tissue repair Heat Shock Protein cell proliferation. |
url |
http://www.ejh.it/index.php/ejh/article/view/2867 |
work_keys_str_mv |
AT andreaamaroli theearthwormdendrobaenavenetaannelidaanewexperimentalorganismforphotobiomodulationandwoundhealing AT saraferrando theearthwormdendrobaenavenetaannelidaanewexperimentalorganismforphotobiomodulationandwoundhealing AT marinapozzolini theearthwormdendrobaenavenetaannelidaanewexperimentalorganismforphotobiomodulationandwoundhealing AT lorenzogallus theearthwormdendrobaenavenetaannelidaanewexperimentalorganismforphotobiomodulationandwoundhealing AT stevenparker theearthwormdendrobaenavenetaannelidaanewexperimentalorganismforphotobiomodulationandwoundhealing AT stefanobenedicenti theearthwormdendrobaenavenetaannelidaanewexperimentalorganismforphotobiomodulationandwoundhealing AT andreaamaroli earthwormdendrobaenavenetaannelidaanewexperimentalorganismforphotobiomodulationandwoundhealing AT saraferrando earthwormdendrobaenavenetaannelidaanewexperimentalorganismforphotobiomodulationandwoundhealing AT marinapozzolini earthwormdendrobaenavenetaannelidaanewexperimentalorganismforphotobiomodulationandwoundhealing AT lorenzogallus earthwormdendrobaenavenetaannelidaanewexperimentalorganismforphotobiomodulationandwoundhealing AT stevenparker earthwormdendrobaenavenetaannelidaanewexperimentalorganismforphotobiomodulationandwoundhealing AT stefanobenedicenti earthwormdendrobaenavenetaannelidaanewexperimentalorganismforphotobiomodulationandwoundhealing |
_version_ |
1724767391469010944 |