Neutrophils kill the parasite Trichomonas vaginalis using trogocytosis.
T. vaginalis, a human-infective parasite, causes the most common nonviral sexually transmitted infection (STI) worldwide and contributes to adverse inflammatory disorders. The immune response to T. vaginalis is poorly understood. Neutrophils (polymorphonuclear cells [PMNs]) are the major immune cell...
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2018-02-01
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Online Access: | https://doi.org/10.1371/journal.pbio.2003885 |
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doaj-6eb27ef1b83d46f2837ef61bc0d1f6682021-07-02T17:07:46ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852018-02-01162e200388510.1371/journal.pbio.2003885Neutrophils kill the parasite Trichomonas vaginalis using trogocytosis.Frances MercerShek Hang NgTaylor M BrownGrace BoatmanPatricia J JohnsonT. vaginalis, a human-infective parasite, causes the most common nonviral sexually transmitted infection (STI) worldwide and contributes to adverse inflammatory disorders. The immune response to T. vaginalis is poorly understood. Neutrophils (polymorphonuclear cells [PMNs]) are the major immune cell present at the T. vaginalis-host interface and are thought to clear T. vaginalis. However, the mechanism of PMN clearance of T. vaginalis has not been characterized. We demonstrate that human PMNs rapidly kill T. vaginalis in a dose-dependent, contact-dependent, and neutrophil extracellular trap (NET)-independent manner. In contrast to phagocytosis, we observed that PMN killing of T. vaginalis involves taking "bites" of T. vaginalis prior to parasite death, using trogocytosis to achieve pathogen killing. Both trogocytosis and parasite killing are dependent on the presence of PMN serine proteases and human serum factors. Our analyses provide the first demonstration, to our knowledge, of a mammalian phagocyte using trogocytosis for pathogen clearance and reveal a novel mechanism used by PMNs to kill a large, highly motile target.https://doi.org/10.1371/journal.pbio.2003885 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Frances Mercer Shek Hang Ng Taylor M Brown Grace Boatman Patricia J Johnson |
spellingShingle |
Frances Mercer Shek Hang Ng Taylor M Brown Grace Boatman Patricia J Johnson Neutrophils kill the parasite Trichomonas vaginalis using trogocytosis. PLoS Biology |
author_facet |
Frances Mercer Shek Hang Ng Taylor M Brown Grace Boatman Patricia J Johnson |
author_sort |
Frances Mercer |
title |
Neutrophils kill the parasite Trichomonas vaginalis using trogocytosis. |
title_short |
Neutrophils kill the parasite Trichomonas vaginalis using trogocytosis. |
title_full |
Neutrophils kill the parasite Trichomonas vaginalis using trogocytosis. |
title_fullStr |
Neutrophils kill the parasite Trichomonas vaginalis using trogocytosis. |
title_full_unstemmed |
Neutrophils kill the parasite Trichomonas vaginalis using trogocytosis. |
title_sort |
neutrophils kill the parasite trichomonas vaginalis using trogocytosis. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS Biology |
issn |
1544-9173 1545-7885 |
publishDate |
2018-02-01 |
description |
T. vaginalis, a human-infective parasite, causes the most common nonviral sexually transmitted infection (STI) worldwide and contributes to adverse inflammatory disorders. The immune response to T. vaginalis is poorly understood. Neutrophils (polymorphonuclear cells [PMNs]) are the major immune cell present at the T. vaginalis-host interface and are thought to clear T. vaginalis. However, the mechanism of PMN clearance of T. vaginalis has not been characterized. We demonstrate that human PMNs rapidly kill T. vaginalis in a dose-dependent, contact-dependent, and neutrophil extracellular trap (NET)-independent manner. In contrast to phagocytosis, we observed that PMN killing of T. vaginalis involves taking "bites" of T. vaginalis prior to parasite death, using trogocytosis to achieve pathogen killing. Both trogocytosis and parasite killing are dependent on the presence of PMN serine proteases and human serum factors. Our analyses provide the first demonstration, to our knowledge, of a mammalian phagocyte using trogocytosis for pathogen clearance and reveal a novel mechanism used by PMNs to kill a large, highly motile target. |
url |
https://doi.org/10.1371/journal.pbio.2003885 |
work_keys_str_mv |
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1721326007224369152 |