Preparation of CaP/pDNA nanoparticles by reverse micro-emulsion method: Optimization of formulation variables using experimental design

Preparation of CaP/pDNA nanoparticles by reverse micro-emulsion method: Optimization of formulation variables using experimental design

In this study, the CaP/pDNA nanoparticles were prepared using Triton X-100/Butanol/Cyclohexane/Water reverse microemulsion system. Optimization of preparation conditions was based on evaluation of particle size by Box–Behnken design method. The particle sizes of the optimized CaP/pDNA nanoparticles...

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Bibliographic Details
Main Authors: Wenpan Li, Shasha Jing, Xiu Xin, Xirui Zhang, Kang Chen, Dawei Chen, Haiyang Hu
Format: Article
Language:English
Published: Elsevier 2017-03-01
Series:Asian Journal of Pharmaceutical Sciences
Subjects:
CaP nanoparticles
pDNA
Reverse microemulsion method
Box–Behnken design
Transfection and expression
Online Access:http://www.sciencedirect.com/science/article/pii/S1818087616301714
Description
Summary:In this study, the CaP/pDNA nanoparticles were prepared using Triton X-100/Butanol/Cyclohexane/Water reverse microemulsion system. Optimization of preparation conditions was based on evaluation of particle size by Box–Behnken design method. The particle sizes of the optimized CaP/pDNA nanoparticles were found to be 60.23 ± 4.72 nm, polydispersity index was 0.252 and pDNA encapsulate efficiency was more than 90%. The optimized CaP/pDNA nanoparticles have pH sensitivity and biocompatibility. Further, optimized CaP/pDNA nanoparticles showed higher transfection efficiency.
ISSN:1818-0876

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