The Potential for Reassortment between Oropouche and Schmallenberg Orthobunyaviruses
A number of viruses within the Peribunyaviridae family are naturally occurring reassortants, a common phenomenon for segmented viruses. Using a minigenome-reporter and virus-like particle (VLP) production assay, we have accessed the potential of Oropouche virus (OROV), Schmallenberg virus (SBV), and...
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doaj-6ebe46e9374046bdb9aa50b2b2f84a8a2020-11-25T00:30:20ZengMDPI AGViruses1999-49152017-08-019822010.3390/v9080220v9080220The Potential for Reassortment between Oropouche and Schmallenberg OrthobunyavirusesNatasha L. Tilston-Lunel0Xiaohong Shi1Richard M. Elliott2Gustavo Olszanski Acrani3MRC-University of Glasgow Centre for Virus Research, 464 Bearsden Road, Glasgow G61 1QH, UKMRC-University of Glasgow Centre for Virus Research, 464 Bearsden Road, Glasgow G61 1QH, UKMRC-University of Glasgow Centre for Virus Research, 464 Bearsden Road, Glasgow G61 1QH, UKUniversidade Federal da Fronteira Sul, Campus Passo Fundo. Rodovia RS 153 - Km 03, Jardim América, CEP 99034-600 Passo Fundo—RS, BrasilA number of viruses within the Peribunyaviridae family are naturally occurring reassortants, a common phenomenon for segmented viruses. Using a minigenome-reporter and virus-like particle (VLP) production assay, we have accessed the potential of Oropouche virus (OROV), Schmallenberg virus (SBV), and other orthobunyaviruses within the Simbu serogroup to reassort. We found that the untranslated region (UTR) in the medium segment is a potential contributing factor for reassortment by the tested viruses. We demonstrate that for promoter activity to occur it was essential that the viral RNA polymerase (L) and nucleocapsid (N) proteins were from the same virus, reinforcing the hypothesis that the large and small segments that encode these proteins segregate together during genome reassortment. Our results indicate that, given the right epidemiological setting, reassortment between SBV and OROV would potentially be feasible and could contribute to the emergence of a new Simbu virus.https://www.mdpi.com/1999-4915/9/8/220Oropouche virusSchmallenberg virusbunyavirusOrthobunyavirusSimbu serogroupminigenomereassortment |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Natasha L. Tilston-Lunel Xiaohong Shi Richard M. Elliott Gustavo Olszanski Acrani |
spellingShingle |
Natasha L. Tilston-Lunel Xiaohong Shi Richard M. Elliott Gustavo Olszanski Acrani The Potential for Reassortment between Oropouche and Schmallenberg Orthobunyaviruses Viruses Oropouche virus Schmallenberg virus bunyavirus Orthobunyavirus Simbu serogroup minigenome reassortment |
author_facet |
Natasha L. Tilston-Lunel Xiaohong Shi Richard M. Elliott Gustavo Olszanski Acrani |
author_sort |
Natasha L. Tilston-Lunel |
title |
The Potential for Reassortment between Oropouche and Schmallenberg Orthobunyaviruses |
title_short |
The Potential for Reassortment between Oropouche and Schmallenberg Orthobunyaviruses |
title_full |
The Potential for Reassortment between Oropouche and Schmallenberg Orthobunyaviruses |
title_fullStr |
The Potential for Reassortment between Oropouche and Schmallenberg Orthobunyaviruses |
title_full_unstemmed |
The Potential for Reassortment between Oropouche and Schmallenberg Orthobunyaviruses |
title_sort |
potential for reassortment between oropouche and schmallenberg orthobunyaviruses |
publisher |
MDPI AG |
series |
Viruses |
issn |
1999-4915 |
publishDate |
2017-08-01 |
description |
A number of viruses within the Peribunyaviridae family are naturally occurring reassortants, a common phenomenon for segmented viruses. Using a minigenome-reporter and virus-like particle (VLP) production assay, we have accessed the potential of Oropouche virus (OROV), Schmallenberg virus (SBV), and other orthobunyaviruses within the Simbu serogroup to reassort. We found that the untranslated region (UTR) in the medium segment is a potential contributing factor for reassortment by the tested viruses. We demonstrate that for promoter activity to occur it was essential that the viral RNA polymerase (L) and nucleocapsid (N) proteins were from the same virus, reinforcing the hypothesis that the large and small segments that encode these proteins segregate together during genome reassortment. Our results indicate that, given the right epidemiological setting, reassortment between SBV and OROV would potentially be feasible and could contribute to the emergence of a new Simbu virus. |
topic |
Oropouche virus Schmallenberg virus bunyavirus Orthobunyavirus Simbu serogroup minigenome reassortment |
url |
https://www.mdpi.com/1999-4915/9/8/220 |
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