Synthesis and Properties of Sulfhydryl-Reactive Near-Infrared Cyanine Fluorochromes for Fluorescence Imaging
Near-infrared fluorochromes (NIRF) are useful compounds for diverse biotechnology applications and for in vivo biomedical imaging. Such NIRF must have high quantum yield, be biocompatible, and be conjugatable to a wide variety of proteins, peptides, and other affinity ligands. Here, we describe the...
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2003-04-01
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Online Access: | https://doi.org/10.1162/15353500200303121 |
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doaj-6ecce7f63d1c4b10b3e55acc222d55572021-04-02T12:54:36ZengHindawi - SAGE PublishingMolecular Imaging1536-01212003-04-01210.1162/1535350020030312110.1162_15353500200303121Synthesis and Properties of Sulfhydryl-Reactive Near-Infrared Cyanine Fluorochromes for Fluorescence ImagingYuhui LinRalph WeisslederChing-Hsuan TungNear-infrared fluorochromes (NIRF) are useful compounds for diverse biotechnology applications and for in vivo biomedical imaging. Such NIRF must have high quantum yield, be biocompatible, and be conjugatable to a wide variety of proteins, peptides, and other affinity ligands. Here, we describe the synthesis of four new nonsymmetrical sulfhydryl-reactive cyanine NIRF with excellent optical and chemical properties. Each fluorochrome was designed to contain an iodoacetamido group that reacts specifically with sulfhydryl-containing molecules. The synthesized fluorochromes were used to label model peptides and sulfhydryl-containing biomolecules.https://doi.org/10.1162/15353500200303121 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yuhui Lin Ralph Weissleder Ching-Hsuan Tung |
spellingShingle |
Yuhui Lin Ralph Weissleder Ching-Hsuan Tung Synthesis and Properties of Sulfhydryl-Reactive Near-Infrared Cyanine Fluorochromes for Fluorescence Imaging Molecular Imaging |
author_facet |
Yuhui Lin Ralph Weissleder Ching-Hsuan Tung |
author_sort |
Yuhui Lin |
title |
Synthesis and Properties of Sulfhydryl-Reactive Near-Infrared Cyanine Fluorochromes for Fluorescence Imaging |
title_short |
Synthesis and Properties of Sulfhydryl-Reactive Near-Infrared Cyanine Fluorochromes for Fluorescence Imaging |
title_full |
Synthesis and Properties of Sulfhydryl-Reactive Near-Infrared Cyanine Fluorochromes for Fluorescence Imaging |
title_fullStr |
Synthesis and Properties of Sulfhydryl-Reactive Near-Infrared Cyanine Fluorochromes for Fluorescence Imaging |
title_full_unstemmed |
Synthesis and Properties of Sulfhydryl-Reactive Near-Infrared Cyanine Fluorochromes for Fluorescence Imaging |
title_sort |
synthesis and properties of sulfhydryl-reactive near-infrared cyanine fluorochromes for fluorescence imaging |
publisher |
Hindawi - SAGE Publishing |
series |
Molecular Imaging |
issn |
1536-0121 |
publishDate |
2003-04-01 |
description |
Near-infrared fluorochromes (NIRF) are useful compounds for diverse biotechnology applications and for in vivo biomedical imaging. Such NIRF must have high quantum yield, be biocompatible, and be conjugatable to a wide variety of proteins, peptides, and other affinity ligands. Here, we describe the synthesis of four new nonsymmetrical sulfhydryl-reactive cyanine NIRF with excellent optical and chemical properties. Each fluorochrome was designed to contain an iodoacetamido group that reacts specifically with sulfhydryl-containing molecules. The synthesized fluorochromes were used to label model peptides and sulfhydryl-containing biomolecules. |
url |
https://doi.org/10.1162/15353500200303121 |
work_keys_str_mv |
AT yuhuilin synthesisandpropertiesofsulfhydrylreactivenearinfraredcyaninefluorochromesforfluorescenceimaging AT ralphweissleder synthesisandpropertiesofsulfhydrylreactivenearinfraredcyaninefluorochromesforfluorescenceimaging AT chinghsuantung synthesisandpropertiesofsulfhydrylreactivenearinfraredcyaninefluorochromesforfluorescenceimaging |
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1721567200105463808 |