MicroRNA-221 promotes papillary thyroid carcinoma cells migration and invasion via targeting RECK and regulating epithelial–mesenchymal transition
Zhao-li Wei,1 Ai-bin Gao,1 Qing Wang,1 Xiu-e Lou,1 Jing Zhao,2 Qing-jun Lu3 1Department of Endocrinology, Binzhou Central Hospital, Binzhou Medical University, Binzhou 251700, Shandong Province, People’s Republic of China; 2Department of Oncology, Provincal Hospital of Shandong University...
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doaj-6ee2e63127bd44eaab49ae3236d14c162020-11-25T01:00:57ZengDove Medical PressOncoTargets and Therapy1178-69302019-03-01Volume 122323233344828MicroRNA-221 promotes papillary thyroid carcinoma cells migration and invasion via targeting RECK and regulating epithelial–mesenchymal transitionWei ZGao AWang QLou XZhao JLu QZhao-li Wei,1 Ai-bin Gao,1 Qing Wang,1 Xiu-e Lou,1 Jing Zhao,2 Qing-jun Lu3 1Department of Endocrinology, Binzhou Central Hospital, Binzhou Medical University, Binzhou 251700, Shandong Province, People’s Republic of China; 2Department of Oncology, Provincal Hospital of Shandong University, Jinan 250000, Shandong Province, People’s Republic of China; 3Department of Oncology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200001, People’s Republic of China Aim: The aim of this study was to detect the effects and potential mechanisms of microRNA-221 on a series of biological behaviors of papillary thyroid carcinoma (PTC) cells in vitro and in vivo. Methods: First, we analyzed the relationship between the expression of miR-221 and several clinicopathological features of PTC patients and then detected the expression of the miR-221 in tumor tissues and cell lines. The effects of miR-221 on proliferation and invasion of PTC cells were verified by cell counting kit-8 (CCK-8) assay, wound healing assay and transwell assay. Western blot assay was applied to explore the correlation between miR-221 and RECK expression in PTC K1 cells. Finally, a xenograft model was established to further confirm the tumor-promoting effects of miR-221 in vivo. Results: Our data indicated that miR-221 was relatively upregulated in metastatic PTC tissues. MiR-221 promoted the proliferation, migration and invasion activities of PTC K1 cells, following variations of epithelial–mesenchymal transition (EMT)-related protein expression. We identified RECK as a direct target of miR-221, revealed its expression to be inversely correlated with miR-221 in PTC samples and showed that its reintroduction reverses miR-221-induced PTC invasiveness. In addition, miR-221 was also verified to promote tumor growth and increase tumor volume and weight in vivo. Taken together, miR-221/RECK axis could be an effective way to regulate biological behaviors of PTC. Conclusion: MiR-221 may be involved in PTC cell invasion and metastasis by targeting RECK, indicating that the miR-221/RECK pathway could be studied further as a potential new diagnostic or prognostic biomarker for PTC. Keywords: miR-221, papillary thyroid carcinoma, invasion, RECKhttps://www.dovepress.com/microrna-221-promotes-papillary-thyroid-carcinoma-cells-migration-and--peer-reviewed-article-OTTPapillary Thyroid CarcinomamiR-221ProliferationMetastasisRECK |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wei Z Gao A Wang Q Lou X Zhao J Lu Q |
spellingShingle |
Wei Z Gao A Wang Q Lou X Zhao J Lu Q MicroRNA-221 promotes papillary thyroid carcinoma cells migration and invasion via targeting RECK and regulating epithelial–mesenchymal transition OncoTargets and Therapy Papillary Thyroid Carcinoma miR-221 Proliferation Metastasis RECK |
author_facet |
Wei Z Gao A Wang Q Lou X Zhao J Lu Q |
author_sort |
Wei Z |
title |
MicroRNA-221 promotes papillary thyroid carcinoma cells migration and invasion via targeting RECK and regulating epithelial–mesenchymal transition |
title_short |
MicroRNA-221 promotes papillary thyroid carcinoma cells migration and invasion via targeting RECK and regulating epithelial–mesenchymal transition |
title_full |
MicroRNA-221 promotes papillary thyroid carcinoma cells migration and invasion via targeting RECK and regulating epithelial–mesenchymal transition |
title_fullStr |
MicroRNA-221 promotes papillary thyroid carcinoma cells migration and invasion via targeting RECK and regulating epithelial–mesenchymal transition |
title_full_unstemmed |
MicroRNA-221 promotes papillary thyroid carcinoma cells migration and invasion via targeting RECK and regulating epithelial–mesenchymal transition |
title_sort |
microrna-221 promotes papillary thyroid carcinoma cells migration and invasion via targeting reck and regulating epithelial–mesenchymal transition |
publisher |
Dove Medical Press |
series |
OncoTargets and Therapy |
issn |
1178-6930 |
publishDate |
2019-03-01 |
description |
Zhao-li Wei,1 Ai-bin Gao,1 Qing Wang,1 Xiu-e Lou,1 Jing Zhao,2 Qing-jun Lu3 1Department of Endocrinology, Binzhou Central Hospital, Binzhou Medical University, Binzhou 251700, Shandong Province, People’s Republic of China; 2Department of Oncology, Provincal Hospital of Shandong University, Jinan 250000, Shandong Province, People’s Republic of China; 3Department of Oncology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200001, People’s Republic of China Aim: The aim of this study was to detect the effects and potential mechanisms of microRNA-221 on a series of biological behaviors of papillary thyroid carcinoma (PTC) cells in vitro and in vivo. Methods: First, we analyzed the relationship between the expression of miR-221 and several clinicopathological features of PTC patients and then detected the expression of the miR-221 in tumor tissues and cell lines. The effects of miR-221 on proliferation and invasion of PTC cells were verified by cell counting kit-8 (CCK-8) assay, wound healing assay and transwell assay. Western blot assay was applied to explore the correlation between miR-221 and RECK expression in PTC K1 cells. Finally, a xenograft model was established to further confirm the tumor-promoting effects of miR-221 in vivo. Results: Our data indicated that miR-221 was relatively upregulated in metastatic PTC tissues. MiR-221 promoted the proliferation, migration and invasion activities of PTC K1 cells, following variations of epithelial–mesenchymal transition (EMT)-related protein expression. We identified RECK as a direct target of miR-221, revealed its expression to be inversely correlated with miR-221 in PTC samples and showed that its reintroduction reverses miR-221-induced PTC invasiveness. In addition, miR-221 was also verified to promote tumor growth and increase tumor volume and weight in vivo. Taken together, miR-221/RECK axis could be an effective way to regulate biological behaviors of PTC. Conclusion: MiR-221 may be involved in PTC cell invasion and metastasis by targeting RECK, indicating that the miR-221/RECK pathway could be studied further as a potential new diagnostic or prognostic biomarker for PTC. Keywords: miR-221, papillary thyroid carcinoma, invasion, RECK |
topic |
Papillary Thyroid Carcinoma miR-221 Proliferation Metastasis RECK |
url |
https://www.dovepress.com/microrna-221-promotes-papillary-thyroid-carcinoma-cells-migration-and--peer-reviewed-article-OTT |
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