Applications of CRISPR/Cas to Improve Crop Disease Resistance: Beyond Inactivation of Susceptibility Factors
Summary: Current crop production systems are prone to increasing pathogen pressure. Fundamental understanding of molecular plant-pathogen interactions, the availability of crop and pathogen genomic information, as well as emerging genome editing permits a novel approach for breeding of crop disease...
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2020-09-01
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Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004220306702 |
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doaj-6efa2df64d25432e9533d25f8818366a2020-11-25T03:27:52ZengElsevieriScience2589-00422020-09-01239101478Applications of CRISPR/Cas to Improve Crop Disease Resistance: Beyond Inactivation of Susceptibility FactorsDirk Schenke0Daguang Cai1Institute of Phytopathology, Department of Molecular Phytopathology and Biotechnology, Christian-Albrechts-University of Kiel, Hermann Rodewald Str. 9, 24118 Kiel, Germany; Corresponding authorInstitute of Phytopathology, Department of Molecular Phytopathology and Biotechnology, Christian-Albrechts-University of Kiel, Hermann Rodewald Str. 9, 24118 Kiel, GermanySummary: Current crop production systems are prone to increasing pathogen pressure. Fundamental understanding of molecular plant-pathogen interactions, the availability of crop and pathogen genomic information, as well as emerging genome editing permits a novel approach for breeding of crop disease resistance. We describe here strategies to identify new targets for resistance breeding with focus on interruption of the compatible plant-pathogen interaction by CRISPR/Cas-mediated genome editing. Basically, crop genome editing can be applied in several ways to achieve this goal. The most common approach focuses on the “simple” knockout by non-homologous end joining repair of plant susceptibility factors required for efficient host colonization. However, genome re-writing via homology-directed repair or base editing can also prevent host manipulation by changing the targets of pathogen-derived effectors or molecules beyond recognition, which also decreases plant susceptibility. We conclude that genome editing by CRISPR/Cas will become increasingly indispensable to generate in relatively short time beneficial resistance traits in crops to meet upcoming challenges.http://www.sciencedirect.com/science/article/pii/S2589004220306702Biological SciencesBiotechnologyPlant BiotechnologyPlant BiologyPlant Genetics |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Dirk Schenke Daguang Cai |
spellingShingle |
Dirk Schenke Daguang Cai Applications of CRISPR/Cas to Improve Crop Disease Resistance: Beyond Inactivation of Susceptibility Factors iScience Biological Sciences Biotechnology Plant Biotechnology Plant Biology Plant Genetics |
author_facet |
Dirk Schenke Daguang Cai |
author_sort |
Dirk Schenke |
title |
Applications of CRISPR/Cas to Improve Crop Disease Resistance: Beyond Inactivation of Susceptibility Factors |
title_short |
Applications of CRISPR/Cas to Improve Crop Disease Resistance: Beyond Inactivation of Susceptibility Factors |
title_full |
Applications of CRISPR/Cas to Improve Crop Disease Resistance: Beyond Inactivation of Susceptibility Factors |
title_fullStr |
Applications of CRISPR/Cas to Improve Crop Disease Resistance: Beyond Inactivation of Susceptibility Factors |
title_full_unstemmed |
Applications of CRISPR/Cas to Improve Crop Disease Resistance: Beyond Inactivation of Susceptibility Factors |
title_sort |
applications of crispr/cas to improve crop disease resistance: beyond inactivation of susceptibility factors |
publisher |
Elsevier |
series |
iScience |
issn |
2589-0042 |
publishDate |
2020-09-01 |
description |
Summary: Current crop production systems are prone to increasing pathogen pressure. Fundamental understanding of molecular plant-pathogen interactions, the availability of crop and pathogen genomic information, as well as emerging genome editing permits a novel approach for breeding of crop disease resistance. We describe here strategies to identify new targets for resistance breeding with focus on interruption of the compatible plant-pathogen interaction by CRISPR/Cas-mediated genome editing. Basically, crop genome editing can be applied in several ways to achieve this goal. The most common approach focuses on the “simple” knockout by non-homologous end joining repair of plant susceptibility factors required for efficient host colonization. However, genome re-writing via homology-directed repair or base editing can also prevent host manipulation by changing the targets of pathogen-derived effectors or molecules beyond recognition, which also decreases plant susceptibility. We conclude that genome editing by CRISPR/Cas will become increasingly indispensable to generate in relatively short time beneficial resistance traits in crops to meet upcoming challenges. |
topic |
Biological Sciences Biotechnology Plant Biotechnology Plant Biology Plant Genetics |
url |
http://www.sciencedirect.com/science/article/pii/S2589004220306702 |
work_keys_str_mv |
AT dirkschenke applicationsofcrisprcastoimprovecropdiseaseresistancebeyondinactivationofsusceptibilityfactors AT daguangcai applicationsofcrisprcastoimprovecropdiseaseresistancebeyondinactivationofsusceptibilityfactors |
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1724586672294723584 |