Chemical composition and enzyme inhibition of Phytolacca dioica L. seeds extracts
Phytolacca, which belongs to the family of Phytolaccaceae, are known for their use in popular medicine. Bioactivity of five extracts from Phytolacca dioica seeds were evaluated in four bioassays. A selected group of compounds from the extract that displayed the best bioactivity was analysed. The eth...
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doaj-6f00e067fb4a42a1bfbf36aaa76923f42020-11-25T02:45:28ZengTaylor & Francis GroupJournal of Enzyme Inhibition and Medicinal Chemistry1475-63661475-63742019-01-0134151952710.1080/14756366.2018.15630771563077Chemical composition and enzyme inhibition of Phytolacca dioica L. seeds extractsAmalia Di Petrillo0Ana María González-Paramás1Antonella Rosa2Valeria Ruggiero3Fabio Boylan4Amit Kumar5Francesca Pintus6Celestino Santos-Buelga7Antonella Fais8Benedetta Era9University of CagliariPolyphenols Research Group. Unit of Nutrition and Bromatology, Faculty of Pharmacy, University of Salamanca, Salamanca, SpainUniversity of CagliariUniversity of CagliariTrinity Biomedical Sciences Institute, Trinity College DublinUniversity of CagliariUniversity of CagliariPolyphenols Research Group. Unit of Nutrition and Bromatology, Faculty of Pharmacy, University of Salamanca, Salamanca, SpainUniversity of CagliariUniversity of CagliariPhytolacca, which belongs to the family of Phytolaccaceae, are known for their use in popular medicine. Bioactivity of five extracts from Phytolacca dioica seeds were evaluated in four bioassays. A selected group of compounds from the extract that displayed the best bioactivity was analysed. The ethyl acetate extract (EAE) possessed the highest content of phenolics, the highest inhibitory activity on the tyrosinase and xanthine oxidase enzymes and showed a high antioxidant activity. HPLC-DAD-MS was employed to identify the phenolics profile of the most active one (EAE). HSCCC analysis of the EAE led to the isolation of phytolaccoside B and a mixture of 4 isomers, isoamericanol B1, B2, C1 and C2. These isoamericanol isomers presented activity against tyrosinase and xanthine oxidase. Our results revealed for the first time an interesting biological activity of the extract and isolated compounds from P. dioica seeds, which could be considered as a source of bioactive molecules.http://dx.doi.org/10.1080/14756366.2018.1563077phytolacca dioicatyrosinasexanthine oxidaseenzyme inhibitionfatty acids |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Amalia Di Petrillo Ana María González-Paramás Antonella Rosa Valeria Ruggiero Fabio Boylan Amit Kumar Francesca Pintus Celestino Santos-Buelga Antonella Fais Benedetta Era |
spellingShingle |
Amalia Di Petrillo Ana María González-Paramás Antonella Rosa Valeria Ruggiero Fabio Boylan Amit Kumar Francesca Pintus Celestino Santos-Buelga Antonella Fais Benedetta Era Chemical composition and enzyme inhibition of Phytolacca dioica L. seeds extracts Journal of Enzyme Inhibition and Medicinal Chemistry phytolacca dioica tyrosinase xanthine oxidase enzyme inhibition fatty acids |
author_facet |
Amalia Di Petrillo Ana María González-Paramás Antonella Rosa Valeria Ruggiero Fabio Boylan Amit Kumar Francesca Pintus Celestino Santos-Buelga Antonella Fais Benedetta Era |
author_sort |
Amalia Di Petrillo |
title |
Chemical composition and enzyme inhibition of Phytolacca dioica L. seeds extracts |
title_short |
Chemical composition and enzyme inhibition of Phytolacca dioica L. seeds extracts |
title_full |
Chemical composition and enzyme inhibition of Phytolacca dioica L. seeds extracts |
title_fullStr |
Chemical composition and enzyme inhibition of Phytolacca dioica L. seeds extracts |
title_full_unstemmed |
Chemical composition and enzyme inhibition of Phytolacca dioica L. seeds extracts |
title_sort |
chemical composition and enzyme inhibition of phytolacca dioica l. seeds extracts |
publisher |
Taylor & Francis Group |
series |
Journal of Enzyme Inhibition and Medicinal Chemistry |
issn |
1475-6366 1475-6374 |
publishDate |
2019-01-01 |
description |
Phytolacca, which belongs to the family of Phytolaccaceae, are known for their use in popular medicine. Bioactivity of five extracts from Phytolacca dioica seeds were evaluated in four bioassays. A selected group of compounds from the extract that displayed the best bioactivity was analysed. The ethyl acetate extract (EAE) possessed the highest content of phenolics, the highest inhibitory activity on the tyrosinase and xanthine oxidase enzymes and showed a high antioxidant activity. HPLC-DAD-MS was employed to identify the phenolics profile of the most active one (EAE). HSCCC analysis of the EAE led to the isolation of phytolaccoside B and a mixture of 4 isomers, isoamericanol B1, B2, C1 and C2. These isoamericanol isomers presented activity against tyrosinase and xanthine oxidase. Our results revealed for the first time an interesting biological activity of the extract and isolated compounds from P. dioica seeds, which could be considered as a source of bioactive molecules. |
topic |
phytolacca dioica tyrosinase xanthine oxidase enzyme inhibition fatty acids |
url |
http://dx.doi.org/10.1080/14756366.2018.1563077 |
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