Insulin In The Carotid Sinus Increases Suprahepatic And Arterial Glucose Levels
Objective: Considering that insulin is a clue hormone in glucose homeostasis, the aim of this study was to analyze the effects of this hormone infused into the isolated carotid sinus (ICS), on suprahepatic and arterial glucose levels. Methods: All procedures were carried out in accordance with the U...
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Centro Nacional de Información de Ciencias Médicas. Editorial de Ciencias Médicas (ECIMED)
2019-05-01
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doaj-6f0ff12b7f874905a23780011eba1c442020-11-25T03:02:52ZspaCentro Nacional de Información de Ciencias Médicas. Editorial de Ciencias Médicas (ECIMED)Revista Cubana de Investigaciones Biomédicas0864-03001561-30112019-05-01381117Insulin In The Carotid Sinus Increases Suprahepatic And Arterial Glucose LevelsMónica Lemus VidalJosé Luis Cadenas FreixasHéctor R. Tejeda ChávezEdgar U. Mejía ChávezJulián E. Martínez SánchezSergio A. Montero VillegasClaudia Z. Valle RubioJosé A. Maturano MelgozaSergio A. Montero CruzElena Roces DorronsoroObjective: Considering that insulin is a clue hormone in glucose homeostasis, the aim of this study was to analyze the effects of this hormone infused into the isolated carotid sinus (ICS), on suprahepatic and arterial glucose levels. Methods: All procedures were carried out in accordance with the United States National Institutes of Health. Ten male Wistar rats 280-300 g anesthetized with sodium pentobarbital (5 mg /100 g i.p.) after 12 h fasting were randomly divided into control and experimental groups. Saline (100µL) or insulin (15 mIU/rat in 100 μL saline) were injected into the ICS. Blood was collected from catheters inserted in the suprahepatic vein (SHV), starting in the jugular vein and into the femoral artery (FA) were placed. Glucose levels were determined at -10 and -5 min before saline or insulin were injected in the ICS; and 1, 5, 10, 20, and 40 min after the above injection. Results:Insulin injection significantly increased glucose levels in the SHV from 128.8 ± 5.2 mg/dL to 207.4 ± 10.6 mg/dL (p = 0.00005), while in the FA they increase from 123.4 ± 6.7 mg/dL to 199.8 ± 9.6 mg/dL(p = 0.0008) at 40 min after insulin injection. Control group with saline did not show significant changes (p = 0.97 in FA) and (p = 0.34 in SHV). The comparison between both groups was significant on arterial (p = 0.007) and venous (p = 0.003) blood glucose levels. Conclusion: As other studies report overactivation of the carotid bodies and sympathetic activity increase after insulin injections in the carotid artery, we assume that insulin in the carotid bodies (CBs)) activates hepatic glycogenolysis to increase blood glucose levels (hyperglycemia).http://www.revibiomedica.sld.cu/index.php/ibi/article/view/102carotid body chemoreceptorsinsulinisolated carotid sinusglycemia |
collection |
DOAJ |
language |
Spanish |
format |
Article |
sources |
DOAJ |
author |
Mónica Lemus Vidal José Luis Cadenas Freixas Héctor R. Tejeda Chávez Edgar U. Mejía Chávez Julián E. Martínez Sánchez Sergio A. Montero Villegas Claudia Z. Valle Rubio José A. Maturano Melgoza Sergio A. Montero Cruz Elena Roces Dorronsoro |
spellingShingle |
Mónica Lemus Vidal José Luis Cadenas Freixas Héctor R. Tejeda Chávez Edgar U. Mejía Chávez Julián E. Martínez Sánchez Sergio A. Montero Villegas Claudia Z. Valle Rubio José A. Maturano Melgoza Sergio A. Montero Cruz Elena Roces Dorronsoro Insulin In The Carotid Sinus Increases Suprahepatic And Arterial Glucose Levels Revista Cubana de Investigaciones Biomédicas carotid body chemoreceptors insulin isolated carotid sinus glycemia |
author_facet |
Mónica Lemus Vidal José Luis Cadenas Freixas Héctor R. Tejeda Chávez Edgar U. Mejía Chávez Julián E. Martínez Sánchez Sergio A. Montero Villegas Claudia Z. Valle Rubio José A. Maturano Melgoza Sergio A. Montero Cruz Elena Roces Dorronsoro |
author_sort |
Mónica Lemus Vidal |
title |
Insulin In The Carotid Sinus Increases Suprahepatic And Arterial Glucose Levels |
title_short |
Insulin In The Carotid Sinus Increases Suprahepatic And Arterial Glucose Levels |
title_full |
Insulin In The Carotid Sinus Increases Suprahepatic And Arterial Glucose Levels |
title_fullStr |
Insulin In The Carotid Sinus Increases Suprahepatic And Arterial Glucose Levels |
title_full_unstemmed |
Insulin In The Carotid Sinus Increases Suprahepatic And Arterial Glucose Levels |
title_sort |
insulin in the carotid sinus increases suprahepatic and arterial glucose levels |
publisher |
Centro Nacional de Información de Ciencias Médicas. Editorial de Ciencias Médicas (ECIMED) |
series |
Revista Cubana de Investigaciones Biomédicas |
issn |
0864-0300 1561-3011 |
publishDate |
2019-05-01 |
description |
Objective: Considering that insulin is a clue hormone in glucose homeostasis, the aim of this study was to analyze the effects of this hormone infused into the isolated carotid sinus (ICS), on suprahepatic and arterial glucose levels. Methods: All procedures were carried out in accordance with the United States National Institutes of Health. Ten male Wistar rats 280-300 g anesthetized with sodium pentobarbital (5 mg /100 g i.p.) after 12 h fasting were randomly divided into control and experimental groups. Saline (100µL) or insulin (15 mIU/rat in 100 μL saline) were injected into the ICS. Blood was collected from catheters inserted in the suprahepatic vein (SHV), starting in the jugular vein and into the femoral artery (FA) were placed. Glucose levels were determined at -10 and -5 min before saline or insulin were injected in the ICS; and 1, 5, 10, 20, and 40 min after the above injection. Results:Insulin injection significantly increased glucose levels in the SHV from 128.8 ± 5.2 mg/dL to 207.4 ± 10.6 mg/dL (p = 0.00005), while in the FA they increase from 123.4 ± 6.7 mg/dL to 199.8 ± 9.6 mg/dL(p = 0.0008) at 40 min after insulin injection. Control group with saline did not show significant changes (p = 0.97 in FA) and (p = 0.34 in SHV). The comparison between both groups was significant on arterial (p = 0.007) and venous (p = 0.003) blood glucose levels. Conclusion: As other studies report overactivation of the carotid bodies and sympathetic activity increase after insulin injections in the carotid artery, we assume that insulin in the carotid bodies (CBs)) activates hepatic glycogenolysis to increase blood glucose levels (hyperglycemia). |
topic |
carotid body chemoreceptors insulin isolated carotid sinus glycemia |
url |
http://www.revibiomedica.sld.cu/index.php/ibi/article/view/102 |
work_keys_str_mv |
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