Epinephrine Biosensor Using Tyrosinase Immobilized Eggshell Membrane

• Main Authors: Sanket Tembe, Sudha Kulkarni, Meena Karve, S. F. D’Souza
• Format: Article
• Language: English
• Published: IFSA Publishing, S.L. 2009-08-01
• Series: Sensors & Transducers
• Subjects: Tyrosinase; Epinephrine; Biopolymers; Electrochemical biosensor
• Online Access: http://www.sensorsportal.com/HTML/DIGEST/august_09/P_480.pdf

Electrochemical biosensor based on immobilized tyrosinase was developed for the determination of epinephrine. The enzyme was extracted from a plant source Amorphophallus companulatus and immobilized on eggshell membrane. Tyrosinase catalyses oxidation of epinephrine to epinephrinequinone which is electrochemically reduced at -0.17 V versus Ag/AgCl (3 M KCl). The resulting current was used for epinephrine quantification. The sensor showed linearity in the range 3 x 10-5 to 3 x 10-4 M with a detection limit of 1 x 10-5 M. It has reusability upto 15 cycles and a shelf life of more than 6 months when stored at 4 o C.