<i>Trichoderma</i> Strains and Metabolites Selectively Increase the Production of Volatile Organic Compounds (VOCs) in Olive Trees
Plants emit volatile organic compounds (VOCs) that induce metabolomic, transcriptomic, and behavioral reactions in receiver organisms, including insect pollinators and herbivores. VOCs’ composition and concentration may influence plant-insect or plant-plant interactions and affect soil microbes that...
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doaj-6f1a3844bb8147e8babb32b2b7dc42802021-03-31T23:03:39ZengMDPI AGMetabolites2218-19892021-03-011121321310.3390/metabo11040213<i>Trichoderma</i> Strains and Metabolites Selectively Increase the Production of Volatile Organic Compounds (VOCs) in Olive TreesIrene Dini0Roberta Marra1Pierpaolo Cavallo2Angela Pironti3Immacolata Sepe4Jacopo Troisi5Giovanni Scala6Pasquale Lombari7Francesco Vinale8Department of Pharmacy, University of Naples Federico II, 80141 Naples, ItalyDepartment of Agricultural Sciences, University of Naples Federico II, Portici, 80055 Naples, ItalyDepartment of Physics “E.R. Caianiello”, University of Salerno, Fisciano, 84084 Salerno, ItalyDepartment of Agricultural Sciences, University of Naples Federico II, Portici, 80055 Naples, ItalyDiagnostica Cavallo S.r.l.—Centro di Ricerca Albo MIUR, 84123 Salerno, ItalyDepartment of Chemistry and Biology “A. Zambelli”, University of Salerno, Baronissi, 84081 Salerno, ItalyTheoreo S.r.l., Montecorvino Pugliano, 84090 Salerno, ItalyDepartment of Agricultural Sciences, University of Naples Federico II, Portici, 80055 Naples, ItalyDepartment of Veterinary Medicine and Animal Productions, University of Naples Federico II, 80138 Naples, ItalyPlants emit volatile organic compounds (VOCs) that induce metabolomic, transcriptomic, and behavioral reactions in receiver organisms, including insect pollinators and herbivores. VOCs’ composition and concentration may influence plant-insect or plant-plant interactions and affect soil microbes that may interfere in plant-plant communication. Many <i>Trichoderma</i> fungi act as biocontrol agents of phytopathogens and plant growth promoters. Moreover, they can stimulate plant defense mechanisms against insect pests. This study evaluated VOCs’ emission by olive trees (<i>Olea europaea</i> L.) when selected <i>Trichoderma</i> fungi or metabolites were used as soil treatments. <i>Trichoderma harzianum</i> strains M10, T22, and TH1, <i>T. asperellum</i> strain KV906, <i>T. virens</i> strain GV41, and their secondary metabolites harzianic acid (HA), and 6-pentyl-α-pyrone (6PP) were applied to olive trees. Charcoal cartridges were employed to adsorb olive VOCs, and gas chromatography mass spectrometry (GC-MS) analysis allowed their identification and quantification. A total of 45 volatile compounds were detected, and among these, twenty-five represented environmental pollutants and nineteen compounds were related to olive plant emission. <i>Trichoderma</i> strains and metabolites differentially enhanced VOCs production, affecting three biosynthetic pathways: methylerythritol 1-phosphate (MEP), lipid-signaling, and shikimate pathways. Multivariate analysis models showed a characteristic fingerprint of each plant-fungus/metabolite relationship, reflecting a different emission of VOCs by the treated plants. Specifically, strain M10 and the metabolites 6PP and HA enhanced the monoterpene syntheses by controlling the MEP pathway. Strains GV41, KV906, and the metabolite HA stimulated the hydrocarbon aldehyde formation (nonanal) by regulating the lipid-signaling pathway. Finally, <i>Trichoderma</i> strains GV41, M10, T22, TH1, and the metabolites HA and 6PP improve aromatic syntheses at different steps of the shikimate pathway.https://www.mdpi.com/2218-1989/11/4/213<i>Olea europaea</i>biocontrol agents<i>Trichoderma</i>secondary metabolitesharzianic acid6-pentyl-α-pyrone |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Irene Dini Roberta Marra Pierpaolo Cavallo Angela Pironti Immacolata Sepe Jacopo Troisi Giovanni Scala Pasquale Lombari Francesco Vinale |
spellingShingle |
Irene Dini Roberta Marra Pierpaolo Cavallo Angela Pironti Immacolata Sepe Jacopo Troisi Giovanni Scala Pasquale Lombari Francesco Vinale <i>Trichoderma</i> Strains and Metabolites Selectively Increase the Production of Volatile Organic Compounds (VOCs) in Olive Trees Metabolites <i>Olea europaea</i> biocontrol agents <i>Trichoderma</i> secondary metabolites harzianic acid 6-pentyl-α-pyrone |
author_facet |
Irene Dini Roberta Marra Pierpaolo Cavallo Angela Pironti Immacolata Sepe Jacopo Troisi Giovanni Scala Pasquale Lombari Francesco Vinale |
author_sort |
Irene Dini |
title |
<i>Trichoderma</i> Strains and Metabolites Selectively Increase the Production of Volatile Organic Compounds (VOCs) in Olive Trees |
title_short |
<i>Trichoderma</i> Strains and Metabolites Selectively Increase the Production of Volatile Organic Compounds (VOCs) in Olive Trees |
title_full |
<i>Trichoderma</i> Strains and Metabolites Selectively Increase the Production of Volatile Organic Compounds (VOCs) in Olive Trees |
title_fullStr |
<i>Trichoderma</i> Strains and Metabolites Selectively Increase the Production of Volatile Organic Compounds (VOCs) in Olive Trees |
title_full_unstemmed |
<i>Trichoderma</i> Strains and Metabolites Selectively Increase the Production of Volatile Organic Compounds (VOCs) in Olive Trees |
title_sort |
<i>trichoderma</i> strains and metabolites selectively increase the production of volatile organic compounds (vocs) in olive trees |
publisher |
MDPI AG |
series |
Metabolites |
issn |
2218-1989 |
publishDate |
2021-03-01 |
description |
Plants emit volatile organic compounds (VOCs) that induce metabolomic, transcriptomic, and behavioral reactions in receiver organisms, including insect pollinators and herbivores. VOCs’ composition and concentration may influence plant-insect or plant-plant interactions and affect soil microbes that may interfere in plant-plant communication. Many <i>Trichoderma</i> fungi act as biocontrol agents of phytopathogens and plant growth promoters. Moreover, they can stimulate plant defense mechanisms against insect pests. This study evaluated VOCs’ emission by olive trees (<i>Olea europaea</i> L.) when selected <i>Trichoderma</i> fungi or metabolites were used as soil treatments. <i>Trichoderma harzianum</i> strains M10, T22, and TH1, <i>T. asperellum</i> strain KV906, <i>T. virens</i> strain GV41, and their secondary metabolites harzianic acid (HA), and 6-pentyl-α-pyrone (6PP) were applied to olive trees. Charcoal cartridges were employed to adsorb olive VOCs, and gas chromatography mass spectrometry (GC-MS) analysis allowed their identification and quantification. A total of 45 volatile compounds were detected, and among these, twenty-five represented environmental pollutants and nineteen compounds were related to olive plant emission. <i>Trichoderma</i> strains and metabolites differentially enhanced VOCs production, affecting three biosynthetic pathways: methylerythritol 1-phosphate (MEP), lipid-signaling, and shikimate pathways. Multivariate analysis models showed a characteristic fingerprint of each plant-fungus/metabolite relationship, reflecting a different emission of VOCs by the treated plants. Specifically, strain M10 and the metabolites 6PP and HA enhanced the monoterpene syntheses by controlling the MEP pathway. Strains GV41, KV906, and the metabolite HA stimulated the hydrocarbon aldehyde formation (nonanal) by regulating the lipid-signaling pathway. Finally, <i>Trichoderma</i> strains GV41, M10, T22, TH1, and the metabolites HA and 6PP improve aromatic syntheses at different steps of the shikimate pathway. |
topic |
<i>Olea europaea</i> biocontrol agents <i>Trichoderma</i> secondary metabolites harzianic acid 6-pentyl-α-pyrone |
url |
https://www.mdpi.com/2218-1989/11/4/213 |
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