2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside-stimulated dental pulp stem cells-derived conditioned medium enhances cell activity and anti-inflammation
Background/purpose: Dental pulp stem cells (DPSCs) contribute to the regeneration of various tissues and have superior proliferation, immune privilege, and anti-inflammation properties to other mesenchymal stem cells. 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside (THSG) not only enhances the afore...
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Elsevier
2021-03-01
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Series: | Journal of Dental Sciences |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S1991790220302373 |
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Article |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yu-tang Chin Che-ming Liu Ting-yi Chen Yao-yu Chung Chi-yu Lin Chao-nan Hsiung Yun-shen Jan Hsien-chung Chiu Earl Fu Sheng-yang Lee |
spellingShingle |
Yu-tang Chin Che-ming Liu Ting-yi Chen Yao-yu Chung Chi-yu Lin Chao-nan Hsiung Yun-shen Jan Hsien-chung Chiu Earl Fu Sheng-yang Lee 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside-stimulated dental pulp stem cells-derived conditioned medium enhances cell activity and anti-inflammation Journal of Dental Sciences Dental pulp stem cell 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside Conditioned medium Proliferation Anti-inflammation Wound healing |
author_facet |
Yu-tang Chin Che-ming Liu Ting-yi Chen Yao-yu Chung Chi-yu Lin Chao-nan Hsiung Yun-shen Jan Hsien-chung Chiu Earl Fu Sheng-yang Lee |
author_sort |
Yu-tang Chin |
title |
2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside-stimulated dental pulp stem cells-derived conditioned medium enhances cell activity and anti-inflammation |
title_short |
2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside-stimulated dental pulp stem cells-derived conditioned medium enhances cell activity and anti-inflammation |
title_full |
2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside-stimulated dental pulp stem cells-derived conditioned medium enhances cell activity and anti-inflammation |
title_fullStr |
2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside-stimulated dental pulp stem cells-derived conditioned medium enhances cell activity and anti-inflammation |
title_full_unstemmed |
2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside-stimulated dental pulp stem cells-derived conditioned medium enhances cell activity and anti-inflammation |
title_sort |
2,3,5,4′-tetrahydroxystilbene-2-o-β-d-glucoside-stimulated dental pulp stem cells-derived conditioned medium enhances cell activity and anti-inflammation |
publisher |
Elsevier |
series |
Journal of Dental Sciences |
issn |
1991-7902 |
publishDate |
2021-03-01 |
description |
Background/purpose: Dental pulp stem cells (DPSCs) contribute to the regeneration of various tissues and have superior proliferation, immune privilege, and anti-inflammation properties to other mesenchymal stem cells. 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside (THSG) not only enhances the aforementioned properties of DPSCs but also promotes self-renewal and reprogramming-like ability. However, whether THSG enhances the aforementioned properties and abilities through direct or indirect interaction mechanisms remains unclear. To address this knowledge gap, we examined the effects of THSG-stimulated DPSC-derived conditioned medium (THSG-CM) on the activity and anti-inflammation properties of cells. Materials and methods: DPSCs were treated with various concentrations of THSG to produce THSG-CM, which was then collected, analyzed, and lyophilized. A cytokine profiling antibody assay was used to compare protein components between THSG-treated and nontreated CM. Human skin fibroblasts (HSFs) and human gingival fibroblasts (HGFs) were used to investigate the effect of THSG-CM on cell proliferation, anti-inflammation, and wound healing abilities; for this investigation, MTS assay, quantitative real-time PCR analysis, and 2-well silicone inserts wound model were conducted. Results: We observed that THSG enhanced the secretion of growth- and immune-associated proteins in THSG-CM and increased the proliferation of HSFs and HGFs. Furthermore, THSG-CM significantly attenuated lipopolysaccharide-stimulated mRNA levels of cytokines in both cells and improved wound healing abilities. Conclusion: We conclude that THSG-CM had more beneficial effects on cell activity and anti-inflammation in the HSFs and HGFs than DPSC-derived CM. DPSC-derived CM can be developed into a cell-free regenerative strategy in the future, and its therapeutic efficacy may be improved by THSG-CM. |
topic |
Dental pulp stem cell 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside Conditioned medium Proliferation Anti-inflammation Wound healing |
url |
http://www.sciencedirect.com/science/article/pii/S1991790220302373 |
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doaj-6f49999dac574d6181fd48c9c34c89c52021-03-27T04:26:58ZengElsevierJournal of Dental Sciences1991-79022021-03-011625865982,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside-stimulated dental pulp stem cells-derived conditioned medium enhances cell activity and anti-inflammationYu-tang Chin0Che-ming Liu1Ting-yi Chen2Yao-yu Chung3Chi-yu Lin4Chao-nan Hsiung5Yun-shen Jan6Hsien-chung Chiu7Earl Fu8Sheng-yang Lee9School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan; Center for Tooth Bank and Dental Stem Cell Technology, Taipei Medical University, Taipei, TaiwanSchool of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan; Department of Dentistry, Wan-Fang Medical Center, Taipei Medical University, Taipei, TaiwanSchool of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan; Department of Dentistry, Wan-Fang Medical Center, Taipei Medical University, Taipei, TaiwanSchool of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, TaiwanSchool of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan; Center for Tooth Bank and Dental Stem Cell Technology, Taipei Medical University, Taipei, TaiwanSchool of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, TaiwanGraduate Institute of Pharmacognosy, College of Pharmacy, Taipei Medical University, Taipei, TaiwanDepartment of Periodontology, School of Dentistry, National Defense Medical Center and Tri-Service General Hospital, Taipei, TaiwanDepartment of Dentistry, Taipei Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Xindian, New Taipei City, TaiwanSchool of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan; Center for Tooth Bank and Dental Stem Cell Technology, Taipei Medical University, Taipei, Taiwan; Department of Dentistry, Wan-Fang Medical Center, Taipei Medical University, Taipei, Taiwan; Corresponding author. School of Dentistry, College of Oral Medicine, Taipei Medical University, 250 Wu-Hsing Street, Taipei, 11031, Taiwan.Background/purpose: Dental pulp stem cells (DPSCs) contribute to the regeneration of various tissues and have superior proliferation, immune privilege, and anti-inflammation properties to other mesenchymal stem cells. 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside (THSG) not only enhances the aforementioned properties of DPSCs but also promotes self-renewal and reprogramming-like ability. However, whether THSG enhances the aforementioned properties and abilities through direct or indirect interaction mechanisms remains unclear. To address this knowledge gap, we examined the effects of THSG-stimulated DPSC-derived conditioned medium (THSG-CM) on the activity and anti-inflammation properties of cells. Materials and methods: DPSCs were treated with various concentrations of THSG to produce THSG-CM, which was then collected, analyzed, and lyophilized. A cytokine profiling antibody assay was used to compare protein components between THSG-treated and nontreated CM. Human skin fibroblasts (HSFs) and human gingival fibroblasts (HGFs) were used to investigate the effect of THSG-CM on cell proliferation, anti-inflammation, and wound healing abilities; for this investigation, MTS assay, quantitative real-time PCR analysis, and 2-well silicone inserts wound model were conducted. Results: We observed that THSG enhanced the secretion of growth- and immune-associated proteins in THSG-CM and increased the proliferation of HSFs and HGFs. Furthermore, THSG-CM significantly attenuated lipopolysaccharide-stimulated mRNA levels of cytokines in both cells and improved wound healing abilities. Conclusion: We conclude that THSG-CM had more beneficial effects on cell activity and anti-inflammation in the HSFs and HGFs than DPSC-derived CM. DPSC-derived CM can be developed into a cell-free regenerative strategy in the future, and its therapeutic efficacy may be improved by THSG-CM.http://www.sciencedirect.com/science/article/pii/S1991790220302373Dental pulp stem cell2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucosideConditioned mediumProliferationAnti-inflammationWound healing |