Postnatal Calvarial Skeletal Stem Cells Expressing PRX1 Reside Exclusively in the Calvarial Sutures and Are Required for Bone Regeneration
Summary: Post-natal skeletal stem cells expressing PRX1 (pnPRX1+) have been identified in the calvaria and in the axial skeleton. Here we characterize the location and functional capacity of the calvarial pnPRX1+ cells. We found that pnPRX1+ reside exclusively in the calvarial suture niche and decre...
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2017-04-01
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doaj-6f7fbc4259aa4f5d8e1f2950b22e169f2020-11-25T00:49:06ZengElsevierStem Cell Reports2213-67112017-04-0184933946Postnatal Calvarial Skeletal Stem Cells Expressing PRX1 Reside Exclusively in the Calvarial Sutures and Are Required for Bone RegenerationKatarzyna Wilk0Shu-Chi A. Yeh1Luke J. Mortensen2Sasan Ghaffarigarakani3Courtney M. Lombardo4Seyed Hossein Bassir5Zahra A. Aldawood6Charles P. Lin7Giuseppe Intini8Department of Oral Medicine, Infection, and Immunity, Harvard School of Dental Medicine, Boston, MA 02115, USADepartment of Oral Medicine, Infection, and Immunity, Harvard School of Dental Medicine, Boston, MA 02115, USA; Advanced Microscopy Program, Center for Systems Biology and Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USADepartment of Oral Medicine, Infection, and Immunity, Harvard School of Dental Medicine, Boston, MA 02115, USA; Advanced Microscopy Program, Center for Systems Biology and Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA; Regenerative Bioscience Center, Rhodes Center for ADS, and College of Engineering, University of Georgia, Athens, GA 30602, USADepartment of Oral Medicine, Infection, and Immunity, Harvard School of Dental Medicine, Boston, MA 02115, USADepartment of Oral Medicine, Infection, and Immunity, Harvard School of Dental Medicine, Boston, MA 02115, USA; University of Florida College of Dentistry, Gainesville, FL 32608, USADepartment of Oral Medicine, Infection, and Immunity, Harvard School of Dental Medicine, Boston, MA 02115, USADepartment of Oral Medicine, Infection, and Immunity, Harvard School of Dental Medicine, Boston, MA 02115, USAAdvanced Microscopy Program, Center for Systems Biology and Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA; Harvard Stem Cell Institute, Cambridge, MA 02138, USA; Corresponding authorDepartment of Oral Medicine, Infection, and Immunity, Harvard School of Dental Medicine, Boston, MA 02115, USA; Harvard Stem Cell Institute, Cambridge, MA 02138, USA; Corresponding authorSummary: Post-natal skeletal stem cells expressing PRX1 (pnPRX1+) have been identified in the calvaria and in the axial skeleton. Here we characterize the location and functional capacity of the calvarial pnPRX1+ cells. We found that pnPRX1+ reside exclusively in the calvarial suture niche and decrease in number with age. They are distinct from preosteoblasts and osteoblasts of the sutures, respond to WNT signaling in vitro and in vivo by differentiating into osteoblasts, and, upon heterotopic transplantation, are able to regenerate bone. Diphtheria toxin A (DTA)-mediated lineage ablation of pnPRX1+ cells and suturectomy perturb regeneration of calvarial bone defects and confirm that pnPRX1+ cells of the sutures are required for bone regeneration. Orthotopic transplantation of sutures with traceable pnPRX1+ cells into wild-type animals shows that pnPRX1+ cells of the suture contribute to calvarial bone defect regeneration. DTA-mediated lineage ablation of pnPRX1+ does not, however, interfere with calvarial development. : Lin, Intini, and colleagues identify the calvarial suture as the stem cell niche for PRX1-expressing postnatal skeletal stem cells by means of intravital microscopy, lineage-tracing, and lineage-ablation experiments. They show that PRX1-expressing cells of the calvaria are required for bone regeneration but are dispensable for calvarial development. Keywords: calvarial skeletal stem cells, stem cell niche, PRX1, PRRX1, calvarial bone regeneration, calvarial developmenthttp://www.sciencedirect.com/science/article/pii/S2213671117300905 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Katarzyna Wilk Shu-Chi A. Yeh Luke J. Mortensen Sasan Ghaffarigarakani Courtney M. Lombardo Seyed Hossein Bassir Zahra A. Aldawood Charles P. Lin Giuseppe Intini |
spellingShingle |
Katarzyna Wilk Shu-Chi A. Yeh Luke J. Mortensen Sasan Ghaffarigarakani Courtney M. Lombardo Seyed Hossein Bassir Zahra A. Aldawood Charles P. Lin Giuseppe Intini Postnatal Calvarial Skeletal Stem Cells Expressing PRX1 Reside Exclusively in the Calvarial Sutures and Are Required for Bone Regeneration Stem Cell Reports |
author_facet |
Katarzyna Wilk Shu-Chi A. Yeh Luke J. Mortensen Sasan Ghaffarigarakani Courtney M. Lombardo Seyed Hossein Bassir Zahra A. Aldawood Charles P. Lin Giuseppe Intini |
author_sort |
Katarzyna Wilk |
title |
Postnatal Calvarial Skeletal Stem Cells Expressing PRX1 Reside Exclusively in the Calvarial Sutures and Are Required for Bone Regeneration |
title_short |
Postnatal Calvarial Skeletal Stem Cells Expressing PRX1 Reside Exclusively in the Calvarial Sutures and Are Required for Bone Regeneration |
title_full |
Postnatal Calvarial Skeletal Stem Cells Expressing PRX1 Reside Exclusively in the Calvarial Sutures and Are Required for Bone Regeneration |
title_fullStr |
Postnatal Calvarial Skeletal Stem Cells Expressing PRX1 Reside Exclusively in the Calvarial Sutures and Are Required for Bone Regeneration |
title_full_unstemmed |
Postnatal Calvarial Skeletal Stem Cells Expressing PRX1 Reside Exclusively in the Calvarial Sutures and Are Required for Bone Regeneration |
title_sort |
postnatal calvarial skeletal stem cells expressing prx1 reside exclusively in the calvarial sutures and are required for bone regeneration |
publisher |
Elsevier |
series |
Stem Cell Reports |
issn |
2213-6711 |
publishDate |
2017-04-01 |
description |
Summary: Post-natal skeletal stem cells expressing PRX1 (pnPRX1+) have been identified in the calvaria and in the axial skeleton. Here we characterize the location and functional capacity of the calvarial pnPRX1+ cells. We found that pnPRX1+ reside exclusively in the calvarial suture niche and decrease in number with age. They are distinct from preosteoblasts and osteoblasts of the sutures, respond to WNT signaling in vitro and in vivo by differentiating into osteoblasts, and, upon heterotopic transplantation, are able to regenerate bone. Diphtheria toxin A (DTA)-mediated lineage ablation of pnPRX1+ cells and suturectomy perturb regeneration of calvarial bone defects and confirm that pnPRX1+ cells of the sutures are required for bone regeneration. Orthotopic transplantation of sutures with traceable pnPRX1+ cells into wild-type animals shows that pnPRX1+ cells of the suture contribute to calvarial bone defect regeneration. DTA-mediated lineage ablation of pnPRX1+ does not, however, interfere with calvarial development. : Lin, Intini, and colleagues identify the calvarial suture as the stem cell niche for PRX1-expressing postnatal skeletal stem cells by means of intravital microscopy, lineage-tracing, and lineage-ablation experiments. They show that PRX1-expressing cells of the calvaria are required for bone regeneration but are dispensable for calvarial development. Keywords: calvarial skeletal stem cells, stem cell niche, PRX1, PRRX1, calvarial bone regeneration, calvarial development |
url |
http://www.sciencedirect.com/science/article/pii/S2213671117300905 |
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