Prevalence of Helicobacter pylori cagA genotype among dyspeptic patients in Southern Thailand

• Main Authors: Sueptrakool Wisessombat, Chatruthai Meethai
• Format: Article
• Language: English
• Published: Wolters Kluwer Medknow Publications 2014-12-01
• Series: Asian Pacific Journal of Reproduction
• Subjects: Helicobacter pylori; Multiplex PCR; Dyspepsia; cagA gene
• Online Access: http://www.sciencedirect.com/science/article/pii/S2305050014600464

Objective: To investigate the prevalence of Helicobacter pylori (H. pylori) infection in dyspepsia patients and its relation to virulence factor cagA gene. Methods: In total, 110 gastric biopsies from dyspeptic patients were comparatively studied using rapid urease test and multiplex PCR. Multiplex PCR detected three genes of 16S rRNA, cagA, and ureC. Results: H. pylori was detected in 14 gastric biopsies (13%). Significantly higher number of female were infected. Furthermore, cagA gene was found in all H. pylori–positive specimens. In addition, the result indicated that the multiplex PCR with annealing temperature at 57 oC was able to effectively amplify specific products. Conclusion: The results confirmed that high preva¬lence of cagA gene in H. pylori among dyspeptic patients in Southern Thailand.