Improving precision of proximity ligation assay by amplified single molecule detection.

Improving precision of proximity ligation assay by amplified single molecule detection.

Proximity ligation assay (PLA) has been proven to be a robust protein detection method. The technique is characterized by high sensitivity and specificity, but the assay precision is probably limited by the PCR readout. To investigate this potential limitation and to improve precision, we developed...

Full description

Bibliographic Details
Main Authors: Rongqin Ke, Rachel Yuan Nong, Simon Fredriksson, Ulf Landegren, Mats Nilsson
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3713053?pdf=render
id doaj-6f9414c9ca614aa098ffc687babfe575
record_format Article
spelling doaj-6f9414c9ca614aa098ffc687babfe5752020-11-24T21:55:22ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0187e6981310.1371/journal.pone.0069813Improving precision of proximity ligation assay by amplified single molecule detection.Rongqin KeRachel Yuan NongSimon FredrikssonUlf LandegrenMats NilssonProximity ligation assay (PLA) has been proven to be a robust protein detection method. The technique is characterized by high sensitivity and specificity, but the assay precision is probably limited by the PCR readout. To investigate this potential limitation and to improve precision, we developed a digital proximity ligation assay for protein measurement in fluids based on amplified single molecule detection. The assay showed significant improvements in precision, and thereby also detection sensitivity, over the conventional real-time PCR readout.http://europepmc.org/articles/PMC3713053?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Rongqin Ke
Rachel Yuan Nong
Simon Fredriksson
Ulf Landegren
Mats Nilsson
spellingShingle Rongqin Ke
Rachel Yuan Nong
Simon Fredriksson
Ulf Landegren
Mats Nilsson
Improving precision of proximity ligation assay by amplified single molecule detection.
PLoS ONE
author_facet Rongqin Ke
Rachel Yuan Nong
Simon Fredriksson
Ulf Landegren
Mats Nilsson
author_sort Rongqin Ke
title Improving precision of proximity ligation assay by amplified single molecule detection.
title_short Improving precision of proximity ligation assay by amplified single molecule detection.
title_full Improving precision of proximity ligation assay by amplified single molecule detection.
title_fullStr Improving precision of proximity ligation assay by amplified single molecule detection.
title_full_unstemmed Improving precision of proximity ligation assay by amplified single molecule detection.
title_sort improving precision of proximity ligation assay by amplified single molecule detection.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2013-01-01
description Proximity ligation assay (PLA) has been proven to be a robust protein detection method. The technique is characterized by high sensitivity and specificity, but the assay precision is probably limited by the PCR readout. To investigate this potential limitation and to improve precision, we developed a digital proximity ligation assay for protein measurement in fluids based on amplified single molecule detection. The assay showed significant improvements in precision, and thereby also detection sensitivity, over the conventional real-time PCR readout.
url http://europepmc.org/articles/PMC3713053?pdf=render
work_keys_str_mv AT rongqinke improvingprecisionofproximityligationassaybyamplifiedsinglemoleculedetection
AT rachelyuannong improvingprecisionofproximityligationassaybyamplifiedsinglemoleculedetection
AT simonfredriksson improvingprecisionofproximityligationassaybyamplifiedsinglemoleculedetection
AT ulflandegren improvingprecisionofproximityligationassaybyamplifiedsinglemoleculedetection
AT matsnilsson improvingprecisionofproximityligationassaybyamplifiedsinglemoleculedetection
_version_ 1725863114556047360

Similar Items