Establishment of two hiPSC lines (IIMCBi001-A and IIMCBi002-A) from dermal fibroblasts of healthy donors and characterization of their cell cycle

Establishment of two hiPSC lines (IIMCBi001-A and IIMCBi002-A) from dermal fibroblasts of healthy donors and characterization of their cell cycle

Two human induced pluripotent stem cell (hiPSC) lines (IIMCBi001-A and IIMCBi002-A) were generated from dermal fibroblasts of healthy females 10 and 30 years old, respectively. For the reprogramming lentiviral vector expressing OCT4, SOX2, KLF4 and C-MYC was used. The generated hiPSCs showed typical...

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Main Authors: Ewa Liszewska, Lena Majchrowicz, Ewelina Krogulec, Katarzyna Kotulska, Leszek Kaczmarek, Katarzyna Kalita, Agnieszka Dobrzyń, Jacek Jaworski
Format: Article
Language:English
Published: Elsevier 2021-04-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506121000714
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spelling doaj-6fa24233c04e49dcafdacf66f8efdedc2021-03-25T04:27:39ZengElsevierStem Cell Research1873-50612021-04-0152102225Establishment of two hiPSC lines (IIMCBi001-A and IIMCBi002-A) from dermal fibroblasts of healthy donors and characterization of their cell cycleEwa Liszewska0Lena Majchrowicz1Ewelina Krogulec2Katarzyna Kotulska3Leszek Kaczmarek4Katarzyna Kalita5Agnieszka Dobrzyń6Jacek Jaworski7Laboratory of Molecular and Cellular Neurobiology, International Institute of Molecular and Cell Biology, Warsaw, Poland; Corresponding author.BRAINCITY, Laboratory of Neurobiology, Nencki Institute of Experimental Biology PAS, Warsaw, PolandLaboratory of Cell Signaling and Metabolic Disorders, Nencki Institute of Experimental Biology PAS, Warsaw, PolandDepartment of Neurology and Epileptology, The Children's Memorial Health Institute, Warsaw, PolandBRAINCITY, Laboratory of Neurobiology, Nencki Institute of Experimental Biology PAS, Warsaw, PolandBRAINCITY, Laboratory of Neurobiology, Nencki Institute of Experimental Biology PAS, Warsaw, PolandLaboratory of Cell Signaling and Metabolic Disorders, Nencki Institute of Experimental Biology PAS, Warsaw, PolandLaboratory of Molecular and Cellular Neurobiology, International Institute of Molecular and Cell Biology, Warsaw, PolandTwo human induced pluripotent stem cell (hiPSC) lines (IIMCBi001-A and IIMCBi002-A) were generated from dermal fibroblasts of healthy females 10 and 30 years old, respectively. For the reprogramming lentiviral vector expressing OCT4, SOX2, KLF4 and C-MYC was used. The generated hiPSCs showed typical embryonic stem cell-like morphology and correct diploid karyotype. Characterization of the hiPSC lines confirmed expression of pluripotency markers and demonstrated their ability to differentiate into the three-germ layers. Cell cycle analysis of the hiPSCs allowed to estimate population doubling time (DT), duration time of particular phases of the cell cycle and proportion of cells found at each phase.http://www.sciencedirect.com/science/article/pii/S1873506121000714
collection DOAJ
language English
format Article
sources DOAJ
author Ewa Liszewska
Lena Majchrowicz
Ewelina Krogulec
Katarzyna Kotulska
Leszek Kaczmarek
Katarzyna Kalita
Agnieszka Dobrzyń
Jacek Jaworski
spellingShingle Ewa Liszewska
Lena Majchrowicz
Ewelina Krogulec
Katarzyna Kotulska
Leszek Kaczmarek
Katarzyna Kalita
Agnieszka Dobrzyń
Jacek Jaworski
Establishment of two hiPSC lines (IIMCBi001-A and IIMCBi002-A) from dermal fibroblasts of healthy donors and characterization of their cell cycle
Stem Cell Research
author_facet Ewa Liszewska
Lena Majchrowicz
Ewelina Krogulec
Katarzyna Kotulska
Leszek Kaczmarek
Katarzyna Kalita
Agnieszka Dobrzyń
Jacek Jaworski
author_sort Ewa Liszewska
title Establishment of two hiPSC lines (IIMCBi001-A and IIMCBi002-A) from dermal fibroblasts of healthy donors and characterization of their cell cycle
title_short Establishment of two hiPSC lines (IIMCBi001-A and IIMCBi002-A) from dermal fibroblasts of healthy donors and characterization of their cell cycle
title_full Establishment of two hiPSC lines (IIMCBi001-A and IIMCBi002-A) from dermal fibroblasts of healthy donors and characterization of their cell cycle
title_fullStr Establishment of two hiPSC lines (IIMCBi001-A and IIMCBi002-A) from dermal fibroblasts of healthy donors and characterization of their cell cycle
title_full_unstemmed Establishment of two hiPSC lines (IIMCBi001-A and IIMCBi002-A) from dermal fibroblasts of healthy donors and characterization of their cell cycle
title_sort establishment of two hipsc lines (iimcbi001-a and iimcbi002-a) from dermal fibroblasts of healthy donors and characterization of their cell cycle
publisher Elsevier
series Stem Cell Research
issn 1873-5061
publishDate 2021-04-01
description Two human induced pluripotent stem cell (hiPSC) lines (IIMCBi001-A and IIMCBi002-A) were generated from dermal fibroblasts of healthy females 10 and 30 years old, respectively. For the reprogramming lentiviral vector expressing OCT4, SOX2, KLF4 and C-MYC was used. The generated hiPSCs showed typical embryonic stem cell-like morphology and correct diploid karyotype. Characterization of the hiPSC lines confirmed expression of pluripotency markers and demonstrated their ability to differentiate into the three-germ layers. Cell cycle analysis of the hiPSCs allowed to estimate population doubling time (DT), duration time of particular phases of the cell cycle and proportion of cells found at each phase.
url http://www.sciencedirect.com/science/article/pii/S1873506121000714
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