Septoria Leaf Blotch and Reduced Nitrogen Availability Alter WRKY Transcription Factor Expression in a Codependent Manner
A major cause of yield loss in wheat worldwide is the fungal pathogen <i>Zymoseptoria tritici</i>, a hemibiotrophic fungus which causes Septoria leaf blotch, the most destructive wheat disease in Europe. Resistance in commercial wheat varieties is poor, however, a link between reduced ni...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2020-06-01
|
Series: | International Journal of Molecular Sciences |
Subjects: | |
Online Access: | https://www.mdpi.com/1422-0067/21/11/4165 |
id |
doaj-6fb20ed18d8a4186b0ace6d252b6f9a9 |
---|---|
record_format |
Article |
spelling |
doaj-6fb20ed18d8a4186b0ace6d252b6f9a92020-11-25T03:03:32ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-06-01214165416510.3390/ijms21114165Septoria Leaf Blotch and Reduced Nitrogen Availability Alter WRKY Transcription Factor Expression in a Codependent MannerAlistair A. Poll0Jack Lee1Roy A. Sanderson2Ed Byrne3John A. Gatehouse4Ari Sadanandom5Angharad M. R. Gatehouse6Martin G. Edwards7School of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne NE1 7RU, UKSchool of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne NE1 7RU, UKSchool of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne NE1 7RU, UKKWS UK Ltd., 56 Church St, Thriplow, Royston SG8 7RE, UKDepartment of Biosciences, Durham University, Durham DH1 3LE, UKDepartment of Biosciences, Durham University, Durham DH1 3LE, UKSchool of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne NE1 7RU, UKSchool of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne NE1 7RU, UKA major cause of yield loss in wheat worldwide is the fungal pathogen <i>Zymoseptoria tritici</i>, a hemibiotrophic fungus which causes Septoria leaf blotch, the most destructive wheat disease in Europe. Resistance in commercial wheat varieties is poor, however, a link between reduced nitrogen availability and increased Septoria tolerance has been observed. We have shown that Septoria load is not affected by nitrogen, whilst the fungus is in its first, symptomless stage of growth. This suggests that a link between nitrogen and Septoria is only present during the necrotrophic phase of Septoria infection. Quantitative real-time PCR data demonstrated that WRKYs, a superfamily of plant-specific transcription factors, are differentially expressed in response to both reduced nitrogen and Septoria. <i>WRKY39</i> was downregulated over 30-fold in response to necrotrophic stage Septoria, whilst changes in the expression of <i>WRKY68a</i> during the late biotrophic phase were dependent on the concentration of nitrogen under which wheat is grown. WRKY68a may therefore mediate a link between nitrogen and Septoria. The potential remains to identify key regulators in the link between nitrogen and Septoria, and as such, elucidate molecular markers for wheat breeding, or targets for molecular-based breeding approaches.https://www.mdpi.com/1422-0067/21/11/4165Septoria leaf blotchstresstranscription factorWRKYwheat<i>Zymoseptoria tritici</i> |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alistair A. Poll Jack Lee Roy A. Sanderson Ed Byrne John A. Gatehouse Ari Sadanandom Angharad M. R. Gatehouse Martin G. Edwards |
spellingShingle |
Alistair A. Poll Jack Lee Roy A. Sanderson Ed Byrne John A. Gatehouse Ari Sadanandom Angharad M. R. Gatehouse Martin G. Edwards Septoria Leaf Blotch and Reduced Nitrogen Availability Alter WRKY Transcription Factor Expression in a Codependent Manner International Journal of Molecular Sciences Septoria leaf blotch stress transcription factor WRKY wheat <i>Zymoseptoria tritici</i> |
author_facet |
Alistair A. Poll Jack Lee Roy A. Sanderson Ed Byrne John A. Gatehouse Ari Sadanandom Angharad M. R. Gatehouse Martin G. Edwards |
author_sort |
Alistair A. Poll |
title |
Septoria Leaf Blotch and Reduced Nitrogen Availability Alter WRKY Transcription Factor Expression in a Codependent Manner |
title_short |
Septoria Leaf Blotch and Reduced Nitrogen Availability Alter WRKY Transcription Factor Expression in a Codependent Manner |
title_full |
Septoria Leaf Blotch and Reduced Nitrogen Availability Alter WRKY Transcription Factor Expression in a Codependent Manner |
title_fullStr |
Septoria Leaf Blotch and Reduced Nitrogen Availability Alter WRKY Transcription Factor Expression in a Codependent Manner |
title_full_unstemmed |
Septoria Leaf Blotch and Reduced Nitrogen Availability Alter WRKY Transcription Factor Expression in a Codependent Manner |
title_sort |
septoria leaf blotch and reduced nitrogen availability alter wrky transcription factor expression in a codependent manner |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2020-06-01 |
description |
A major cause of yield loss in wheat worldwide is the fungal pathogen <i>Zymoseptoria tritici</i>, a hemibiotrophic fungus which causes Septoria leaf blotch, the most destructive wheat disease in Europe. Resistance in commercial wheat varieties is poor, however, a link between reduced nitrogen availability and increased Septoria tolerance has been observed. We have shown that Septoria load is not affected by nitrogen, whilst the fungus is in its first, symptomless stage of growth. This suggests that a link between nitrogen and Septoria is only present during the necrotrophic phase of Septoria infection. Quantitative real-time PCR data demonstrated that WRKYs, a superfamily of plant-specific transcription factors, are differentially expressed in response to both reduced nitrogen and Septoria. <i>WRKY39</i> was downregulated over 30-fold in response to necrotrophic stage Septoria, whilst changes in the expression of <i>WRKY68a</i> during the late biotrophic phase were dependent on the concentration of nitrogen under which wheat is grown. WRKY68a may therefore mediate a link between nitrogen and Septoria. The potential remains to identify key regulators in the link between nitrogen and Septoria, and as such, elucidate molecular markers for wheat breeding, or targets for molecular-based breeding approaches. |
topic |
Septoria leaf blotch stress transcription factor WRKY wheat <i>Zymoseptoria tritici</i> |
url |
https://www.mdpi.com/1422-0067/21/11/4165 |
work_keys_str_mv |
AT alistairapoll septorialeafblotchandreducednitrogenavailabilityalterwrkytranscriptionfactorexpressioninacodependentmanner AT jacklee septorialeafblotchandreducednitrogenavailabilityalterwrkytranscriptionfactorexpressioninacodependentmanner AT royasanderson septorialeafblotchandreducednitrogenavailabilityalterwrkytranscriptionfactorexpressioninacodependentmanner AT edbyrne septorialeafblotchandreducednitrogenavailabilityalterwrkytranscriptionfactorexpressioninacodependentmanner AT johnagatehouse septorialeafblotchandreducednitrogenavailabilityalterwrkytranscriptionfactorexpressioninacodependentmanner AT arisadanandom septorialeafblotchandreducednitrogenavailabilityalterwrkytranscriptionfactorexpressioninacodependentmanner AT angharadmrgatehouse septorialeafblotchandreducednitrogenavailabilityalterwrkytranscriptionfactorexpressioninacodependentmanner AT martingedwards septorialeafblotchandreducednitrogenavailabilityalterwrkytranscriptionfactorexpressioninacodependentmanner |
_version_ |
1724685301966700544 |