Integrated Amplification Microarrays for Infectious Disease Diagnostics
This overview describes microarray-based tests that combine solution-phase amplification chemistry and microarray hybridization within a single microfluidic chamber. The integrated biochemical approach improves microarray workflow for diagnostic applications by reducing the number of steps and minim...
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doaj-6fb3faf8a7b04533b1d83417a280797b2020-11-24T21:54:51ZengMDPI AGMicroarrays2076-39052012-11-011310712410.3390/microarrays1030107Integrated Amplification Microarrays for Infectious Disease DiagnosticsDarrell P. ChandlerLexi BryantSara B. GriesemerRui GuChristopher KnickerbockerAlexander KukhtinJennifer ParkerCynthia ZimmermanKirsten St. GeorgeChristopher G. CooneyThis overview describes microarray-based tests that combine solution-phase amplification chemistry and microarray hybridization within a single microfluidic chamber. The integrated biochemical approach improves microarray workflow for diagnostic applications by reducing the number of steps and minimizing the potential for sample or amplicon cross-contamination. Examples described herein illustrate a basic, integrated approach for DNA and RNA genomes, and a simple consumable architecture for incorporating wash steps while retaining an entirely closed system. It is anticipated that integrated microarray biochemistry will provide an opportunity to significantly reduce the complexity and cost of microarray consumables, equipment, and workflow, which in turn will enable a broader spectrum of users to exploit the intrinsic multiplexing power of microarrays for infectious disease diagnostics.http://www.mdpi.com/2076-3905/1/3/107microfluidicsdiagnosticsgel element arraysasymmetric PCRRT-PCRreverse transcriptasemultiplexintegrated microarrays |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Darrell P. Chandler Lexi Bryant Sara B. Griesemer Rui Gu Christopher Knickerbocker Alexander Kukhtin Jennifer Parker Cynthia Zimmerman Kirsten St. George Christopher G. Cooney |
spellingShingle |
Darrell P. Chandler Lexi Bryant Sara B. Griesemer Rui Gu Christopher Knickerbocker Alexander Kukhtin Jennifer Parker Cynthia Zimmerman Kirsten St. George Christopher G. Cooney Integrated Amplification Microarrays for Infectious Disease Diagnostics Microarrays microfluidics diagnostics gel element arrays asymmetric PCR RT-PCR reverse transcriptase multiplex integrated microarrays |
author_facet |
Darrell P. Chandler Lexi Bryant Sara B. Griesemer Rui Gu Christopher Knickerbocker Alexander Kukhtin Jennifer Parker Cynthia Zimmerman Kirsten St. George Christopher G. Cooney |
author_sort |
Darrell P. Chandler |
title |
Integrated Amplification Microarrays for Infectious Disease Diagnostics |
title_short |
Integrated Amplification Microarrays for Infectious Disease Diagnostics |
title_full |
Integrated Amplification Microarrays for Infectious Disease Diagnostics |
title_fullStr |
Integrated Amplification Microarrays for Infectious Disease Diagnostics |
title_full_unstemmed |
Integrated Amplification Microarrays for Infectious Disease Diagnostics |
title_sort |
integrated amplification microarrays for infectious disease diagnostics |
publisher |
MDPI AG |
series |
Microarrays |
issn |
2076-3905 |
publishDate |
2012-11-01 |
description |
This overview describes microarray-based tests that combine solution-phase amplification chemistry and microarray hybridization within a single microfluidic chamber. The integrated biochemical approach improves microarray workflow for diagnostic applications by reducing the number of steps and minimizing the potential for sample or amplicon cross-contamination. Examples described herein illustrate a basic, integrated approach for DNA and RNA genomes, and a simple consumable architecture for incorporating wash steps while retaining an entirely closed system. It is anticipated that integrated microarray biochemistry will provide an opportunity to significantly reduce the complexity and cost of microarray consumables, equipment, and workflow, which in turn will enable a broader spectrum of users to exploit the intrinsic multiplexing power of microarrays for infectious disease diagnostics. |
topic |
microfluidics diagnostics gel element arrays asymmetric PCR RT-PCR reverse transcriptase multiplex integrated microarrays |
url |
http://www.mdpi.com/2076-3905/1/3/107 |
work_keys_str_mv |
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1725865307455619072 |