Development of a mass spectrometric method to quantitate platelet activating factor in mouse urine.

Development of a mass spectrometric method to quantitate platelet activating factor in mouse urine.

Platelet activating factor (PAF) is a lipid mediator of inflammation released by a variety of stimulated inflammatory cells. It may be involved in immune glomerulonephritis. Thus, its measurement in urine could give information on the mechanism of this disease. We present here a method to measure PA...

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Main Authors: E Benfenati, D Macconi, M Noris, G Icardi, L Bettazzoli, G De Bellis, M Gavinelli, S Rotondo, G Remuzzi
Format: Article
Language:English
Published: Elsevier 1989-12-01
Series:Journal of Lipid Research
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520382092
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spelling doaj-6fc7429de0ca4fe396181f94098d61472021-04-25T04:17:50ZengElsevierJournal of Lipid Research0022-22751989-12-01301219771981Development of a mass spectrometric method to quantitate platelet activating factor in mouse urine.E Benfenati0D Macconi1M Noris2G Icardi3L Bettazzoli4G De Bellis5M Gavinelli6S Rotondo7G Remuzzi8Istituto di Richerche Farmacologiche Mario Negri, Bergamo, Italy.Istituto di Richerche Farmacologiche Mario Negri, Bergamo, Italy.Istituto di Richerche Farmacologiche Mario Negri, Bergamo, Italy.Istituto di Richerche Farmacologiche Mario Negri, Bergamo, Italy.Istituto di Richerche Farmacologiche Mario Negri, Bergamo, Italy.Istituto di Richerche Farmacologiche Mario Negri, Bergamo, Italy.Istituto di Richerche Farmacologiche Mario Negri, Bergamo, Italy.Istituto di Richerche Farmacologiche Mario Negri, Bergamo, Italy.Istituto di Richerche Farmacologiche Mario Negri, Bergamo, Italy.Platelet activating factor (PAF) is a lipid mediator of inflammation released by a variety of stimulated inflammatory cells. It may be involved in immune glomerulonephritis. Thus, its measurement in urine could give information on the mechanism of this disease. We present here a method to measure PAF in mouse urine, using gas-liquid chromatography-mass spectrometry (GLC-MS) in the selected ion recording (SIR) mode. Before instrumental analysis, the extracted and purified samples were hydrolyzed and derivatized with pentafluorobenzoyl chloride. Different experimental conditions are presented and discussed to corroborate the analytical findings. PAF levels in mouse urine were 2.08 +/- 0.46 ng/24 h. This procedure might represent a new experimental tool to establish the possible role of PAF as mediator of tissue damage in renal disease.http://www.sciencedirect.com/science/article/pii/S0022227520382092
collection DOAJ
language English
format Article
sources DOAJ
author E Benfenati
D Macconi
M Noris
G Icardi
L Bettazzoli
G De Bellis
M Gavinelli
S Rotondo
G Remuzzi
spellingShingle E Benfenati
D Macconi
M Noris
G Icardi
L Bettazzoli
G De Bellis
M Gavinelli
S Rotondo
G Remuzzi
Development of a mass spectrometric method to quantitate platelet activating factor in mouse urine.
Journal of Lipid Research
author_facet E Benfenati
D Macconi
M Noris
G Icardi
L Bettazzoli
G De Bellis
M Gavinelli
S Rotondo
G Remuzzi
author_sort E Benfenati
title Development of a mass spectrometric method to quantitate platelet activating factor in mouse urine.
title_short Development of a mass spectrometric method to quantitate platelet activating factor in mouse urine.
title_full Development of a mass spectrometric method to quantitate platelet activating factor in mouse urine.
title_fullStr Development of a mass spectrometric method to quantitate platelet activating factor in mouse urine.
title_full_unstemmed Development of a mass spectrometric method to quantitate platelet activating factor in mouse urine.
title_sort development of a mass spectrometric method to quantitate platelet activating factor in mouse urine.
publisher Elsevier
series Journal of Lipid Research
issn 0022-2275
publishDate 1989-12-01
description Platelet activating factor (PAF) is a lipid mediator of inflammation released by a variety of stimulated inflammatory cells. It may be involved in immune glomerulonephritis. Thus, its measurement in urine could give information on the mechanism of this disease. We present here a method to measure PAF in mouse urine, using gas-liquid chromatography-mass spectrometry (GLC-MS) in the selected ion recording (SIR) mode. Before instrumental analysis, the extracted and purified samples were hydrolyzed and derivatized with pentafluorobenzoyl chloride. Different experimental conditions are presented and discussed to corroborate the analytical findings. PAF levels in mouse urine were 2.08 +/- 0.46 ng/24 h. This procedure might represent a new experimental tool to establish the possible role of PAF as mediator of tissue damage in renal disease.
url http://www.sciencedirect.com/science/article/pii/S0022227520382092
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