Decrease in intramuscular lipid droplets and translocation of HSL in response to muscle contraction and epinephrine
A better understanding of skeletal muscle lipid metabolism is needed to identify the molecular mechanisms relating intramuscular triglyceride (IMTG) to muscle metabolism and insulin sensitivity. An increasing number of proteins have been reported to be associated with intracellular triglyceride (TG)...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2006-11-01
|
Series: | Journal of Lipid Research |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520433759 |
id |
doaj-6fdffc1e15fa4915a7bb19d68b1ed797 |
---|---|
record_format |
Article |
spelling |
doaj-6fdffc1e15fa4915a7bb19d68b1ed7972021-04-27T04:47:24ZengElsevierJournal of Lipid Research0022-22752006-11-01471123922399Decrease in intramuscular lipid droplets and translocation of HSL in response to muscle contraction and epinephrineClara Prats0Morten Donsmark1Klaus Qvortrup2Constantine Londos3Carole Sztalryd4Cecilia Holm5Henrik Galbo6Thorkil Ploug7Copenhagen Muscle Research Center, Department of Medical Physiology, Panum Institute, University of Copenhagen, DK-2200 Copenhagen N, DenmarkCopenhagen Muscle Research Center, Department of Medical Physiology, Panum Institute, University of Copenhagen, DK-2200 Copenhagen N, DenmarkDepartment of Medical Anatomy, Panum Institute, University of Copenhagen, DK-2200 Copenhagen N, DenmarkLaboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-8028Geriatric Research, Education, and Clinical Center, Baltimore Veterans Affairs Health Care Center, Department of Medicine, School of Medicine, University of Maryland, Baltimore, MD 21201Department of Experimental Medical Science, University of Lund, BMC C11, SE-221 84, Lund, SwedenCopenhagen Muscle Research Center, Department of Rheumatology, Bispebjerg Hospital, DK-2400 Copenhagen NV, DenmarkCopenhagen Muscle Research Center, Department of Medical Physiology, Panum Institute, University of Copenhagen, DK-2200 Copenhagen N, DenmarkA better understanding of skeletal muscle lipid metabolism is needed to identify the molecular mechanisms relating intramuscular triglyceride (IMTG) to muscle metabolism and insulin sensitivity. An increasing number of proteins have been reported to be associated with intracellular triglyceride (TG), among them the PAT family members: perilipin, ADRP (for adipocyte differentiation-related protein), and TIP47 (for tail-interacting protein of 47 kDa). Hormone-sensitive lipase (HSL) is thought to be the major enzyme responsible for IMTG hydrolysis in skeletal muscle. In adipocytes, regulation of HSL by intracellular redistribution has been demonstrated. The existence of such regulatory mechanisms in skeletal muscle has long been hypothesized but has never been demonstrated. The aim of this study was to characterize the PAT family proteins associated with IMTG and to investigate the effect of epinephrine stimulation or muscle contraction on skeletal muscle TG content and HSL intracellular distribution. Rat soleus muscles were either incubated with epinephrine or electrically stimulated for 15 min. Single muscle fibers were used for morphological analysis by confocal and transmission electron microscopy. We show a decrease in IMTG in response to both lipolytic stimuli. Furthermore, we identify two PAT family proteins, ADRP and TIP47, associated with IMTG. Finally, we demonstrate HSL translocation to IMTG and ADRP after stimulation with epinephrine or contraction.http://www.sciencedirect.com/science/article/pii/S0022227520433759lipid metabolism regulationskeletal musclehormone-sensitive lipaseadipocyte differentiation-related proteintail-interacting protein of 47 kDa |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Clara Prats Morten Donsmark Klaus Qvortrup Constantine Londos Carole Sztalryd Cecilia Holm Henrik Galbo Thorkil Ploug |
spellingShingle |
Clara Prats Morten Donsmark Klaus Qvortrup Constantine Londos Carole Sztalryd Cecilia Holm Henrik Galbo Thorkil Ploug Decrease in intramuscular lipid droplets and translocation of HSL in response to muscle contraction and epinephrine Journal of Lipid Research lipid metabolism regulation skeletal muscle hormone-sensitive lipase adipocyte differentiation-related protein tail-interacting protein of 47 kDa |
author_facet |
Clara Prats Morten Donsmark Klaus Qvortrup Constantine Londos Carole Sztalryd Cecilia Holm Henrik Galbo Thorkil Ploug |
author_sort |
Clara Prats |
title |
Decrease in intramuscular lipid droplets and translocation of HSL in response to muscle contraction and epinephrine |
title_short |
Decrease in intramuscular lipid droplets and translocation of HSL in response to muscle contraction and epinephrine |
title_full |
Decrease in intramuscular lipid droplets and translocation of HSL in response to muscle contraction and epinephrine |
title_fullStr |
Decrease in intramuscular lipid droplets and translocation of HSL in response to muscle contraction and epinephrine |
title_full_unstemmed |
Decrease in intramuscular lipid droplets and translocation of HSL in response to muscle contraction and epinephrine |
title_sort |
decrease in intramuscular lipid droplets and translocation of hsl in response to muscle contraction and epinephrine |
publisher |
Elsevier |
series |
Journal of Lipid Research |
issn |
0022-2275 |
publishDate |
2006-11-01 |
description |
A better understanding of skeletal muscle lipid metabolism is needed to identify the molecular mechanisms relating intramuscular triglyceride (IMTG) to muscle metabolism and insulin sensitivity. An increasing number of proteins have been reported to be associated with intracellular triglyceride (TG), among them the PAT family members: perilipin, ADRP (for adipocyte differentiation-related protein), and TIP47 (for tail-interacting protein of 47 kDa). Hormone-sensitive lipase (HSL) is thought to be the major enzyme responsible for IMTG hydrolysis in skeletal muscle. In adipocytes, regulation of HSL by intracellular redistribution has been demonstrated. The existence of such regulatory mechanisms in skeletal muscle has long been hypothesized but has never been demonstrated. The aim of this study was to characterize the PAT family proteins associated with IMTG and to investigate the effect of epinephrine stimulation or muscle contraction on skeletal muscle TG content and HSL intracellular distribution. Rat soleus muscles were either incubated with epinephrine or electrically stimulated for 15 min. Single muscle fibers were used for morphological analysis by confocal and transmission electron microscopy. We show a decrease in IMTG in response to both lipolytic stimuli. Furthermore, we identify two PAT family proteins, ADRP and TIP47, associated with IMTG. Finally, we demonstrate HSL translocation to IMTG and ADRP after stimulation with epinephrine or contraction. |
topic |
lipid metabolism regulation skeletal muscle hormone-sensitive lipase adipocyte differentiation-related protein tail-interacting protein of 47 kDa |
url |
http://www.sciencedirect.com/science/article/pii/S0022227520433759 |
work_keys_str_mv |
AT claraprats decreaseinintramuscularlipiddropletsandtranslocationofhslinresponsetomusclecontractionandepinephrine AT mortendonsmark decreaseinintramuscularlipiddropletsandtranslocationofhslinresponsetomusclecontractionandepinephrine AT klausqvortrup decreaseinintramuscularlipiddropletsandtranslocationofhslinresponsetomusclecontractionandepinephrine AT constantinelondos decreaseinintramuscularlipiddropletsandtranslocationofhslinresponsetomusclecontractionandepinephrine AT carolesztalryd decreaseinintramuscularlipiddropletsandtranslocationofhslinresponsetomusclecontractionandepinephrine AT ceciliaholm decreaseinintramuscularlipiddropletsandtranslocationofhslinresponsetomusclecontractionandepinephrine AT henrikgalbo decreaseinintramuscularlipiddropletsandtranslocationofhslinresponsetomusclecontractionandepinephrine AT thorkilploug decreaseinintramuscularlipiddropletsandtranslocationofhslinresponsetomusclecontractionandepinephrine |
_version_ |
1721506347071045632 |