Genetic and biological characterisation of Zika virus isolates from different Brazilian regions
BACKGROUND Zika virus (ZIKV) infections reported in recent epidemics have been linked to clinical complications that had never been associated with ZIKV before. Adaptive mutations could have contributed to the successful emergence of ZIKV as a global health threat to a nonimmune population. However...
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doaj-6fef69296d2c4fde81fcc3865b72dc572020-11-25T01:59:06ZengInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz.1678-806011410.1590/0074-02760190150S0074-02762019000100345Genetic and biological characterisation of Zika virus isolates from different Brazilian regionsDaisy Maria StrottmannCamila ZanlucaAna Luiza Pamplona MosimannAndrea C KoishiNathalia Cavalheiro AuwerterHelisson FaoroAllan Henrique Depieri CataneoDiogo KuczeraPryscilla Fanini WowkJuliano BordignonClaudia Nunes Duarte dos SantosBACKGROUND Zika virus (ZIKV) infections reported in recent epidemics have been linked to clinical complications that had never been associated with ZIKV before. Adaptive mutations could have contributed to the successful emergence of ZIKV as a global health threat to a nonimmune population. However, the causal relationships between the ZIKV genetic determinants, the pathogenesis and the rapid spread in Latin America and in the Caribbean remain widely unknown. OBJECTIVES The aim of this study was to characterise three ZIKV isolates obtained from patient samples during the 2015/2016 Brazilian epidemics. METHODS The ZIKV genomes of these strains were completely sequenced and in vitro infection kinetics experiments were carried out in cell lines and human primary cells. FINDINGS Eight nonsynonymous substitutions throughout the viral genome of the three Brazilian isolates were identified. Infection kinetics experiments were carried out with mammalian cell lines A549, Huh7.5, Vero E6 and human monocyte-derived dendritic cells (mdDCs) and insect cells (Aag2, C6/36 and AP61) and suggest that some of these mutations might be associated with distinct viral fitness. The clinical isolates also presented differences in their infectivity rates when compared to the well-established ZIKV strains (MR766 and PE243), especially in their abilities to infect mammalian cells. MAIN CONCLUSIONS Genomic analysis of three recent ZIKV isolates revealed some nonsynonymous substitutions, which could have an impact on the viral fitness in mammalian and insect cells.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762019000100345&lng=en&tlng=enZika virusFlavivirusmolecular markersbiological characterisation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Daisy Maria Strottmann Camila Zanluca Ana Luiza Pamplona Mosimann Andrea C Koishi Nathalia Cavalheiro Auwerter Helisson Faoro Allan Henrique Depieri Cataneo Diogo Kuczera Pryscilla Fanini Wowk Juliano Bordignon Claudia Nunes Duarte dos Santos |
spellingShingle |
Daisy Maria Strottmann Camila Zanluca Ana Luiza Pamplona Mosimann Andrea C Koishi Nathalia Cavalheiro Auwerter Helisson Faoro Allan Henrique Depieri Cataneo Diogo Kuczera Pryscilla Fanini Wowk Juliano Bordignon Claudia Nunes Duarte dos Santos Genetic and biological characterisation of Zika virus isolates from different Brazilian regions Memórias do Instituto Oswaldo Cruz. Zika virus Flavivirus molecular markers biological characterisation |
author_facet |
Daisy Maria Strottmann Camila Zanluca Ana Luiza Pamplona Mosimann Andrea C Koishi Nathalia Cavalheiro Auwerter Helisson Faoro Allan Henrique Depieri Cataneo Diogo Kuczera Pryscilla Fanini Wowk Juliano Bordignon Claudia Nunes Duarte dos Santos |
author_sort |
Daisy Maria Strottmann |
title |
Genetic and biological characterisation of Zika virus isolates from different Brazilian regions |
title_short |
Genetic and biological characterisation of Zika virus isolates from different Brazilian regions |
title_full |
Genetic and biological characterisation of Zika virus isolates from different Brazilian regions |
title_fullStr |
Genetic and biological characterisation of Zika virus isolates from different Brazilian regions |
title_full_unstemmed |
Genetic and biological characterisation of Zika virus isolates from different Brazilian regions |
title_sort |
genetic and biological characterisation of zika virus isolates from different brazilian regions |
publisher |
Instituto Oswaldo Cruz, Ministério da Saúde |
series |
Memórias do Instituto Oswaldo Cruz. |
issn |
1678-8060 |
description |
BACKGROUND Zika virus (ZIKV) infections reported in recent epidemics have been linked to clinical complications that had never been associated with ZIKV before. Adaptive mutations could have contributed to the successful emergence of ZIKV as a global health threat to a nonimmune population. However, the causal relationships between the ZIKV genetic determinants, the pathogenesis and the rapid spread in Latin America and in the Caribbean remain widely unknown. OBJECTIVES The aim of this study was to characterise three ZIKV isolates obtained from patient samples during the 2015/2016 Brazilian epidemics. METHODS The ZIKV genomes of these strains were completely sequenced and in vitro infection kinetics experiments were carried out in cell lines and human primary cells. FINDINGS Eight nonsynonymous substitutions throughout the viral genome of the three Brazilian isolates were identified. Infection kinetics experiments were carried out with mammalian cell lines A549, Huh7.5, Vero E6 and human monocyte-derived dendritic cells (mdDCs) and insect cells (Aag2, C6/36 and AP61) and suggest that some of these mutations might be associated with distinct viral fitness. The clinical isolates also presented differences in their infectivity rates when compared to the well-established ZIKV strains (MR766 and PE243), especially in their abilities to infect mammalian cells. MAIN CONCLUSIONS Genomic analysis of three recent ZIKV isolates revealed some nonsynonymous substitutions, which could have an impact on the viral fitness in mammalian and insect cells. |
topic |
Zika virus Flavivirus molecular markers biological characterisation |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762019000100345&lng=en&tlng=en |
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