Amelioration of CCl4-Induced Hepatotoxicity in Rabbits by Lepidium sativum Seeds
The current study aimed to evaluate the probable protective effect of Lepidium sativum seeds (LSS) against CCl4 induced hepatic injury in New-Zealand rabbits. Rabbits were randomly divided into two main groups; group-A (noninjured group, n=15) was divided to subgroups A1 (untreated control) and A2 a...
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Online Access: | http://dx.doi.org/10.1155/2019/5947234 |
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doaj-70092cbcc8da4d5b8f94974986026e7b2020-11-25T00:13:52ZengHindawi LimitedEvidence-Based Complementary and Alternative Medicine1741-427X1741-42882019-01-01201910.1155/2019/59472345947234Amelioration of CCl4-Induced Hepatotoxicity in Rabbits by Lepidium sativum SeedsMazin A. Zamzami0Othman A. S. Baothman1Fatma Samy2Mohamed Kamel Abo-Golayel3Biochemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi ArabiaBiochemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi ArabiaPathology Department, Faculty of Medicine, Ain Shams University, Cairo, EgyptBiochemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi ArabiaThe current study aimed to evaluate the probable protective effect of Lepidium sativum seeds (LSS) against CCl4 induced hepatic injury in New-Zealand rabbits. Rabbits were randomly divided into two main groups; group-A (noninjured group, n=15) was divided to subgroups A1 (untreated control) and A2 and A3 which received 200 & 400 mg/kg bw of LSS, respectively, in their diet daily. Group-B (injured group, n=30) were subcutaneously injected with CCl4 (0.5 ml/kg bw) starting from day one of the experiment and were equally divided into 3 subgroups: B1 received normal standard diet and B2 & B3 received 200 & 400 mg/kg bw of LSS, respectively, in their diet daily. Five rabbits of all subgroups were decapitated 5 and 10 weeks after experimental running. Biochemical analysis revealed significant decrease in serum levels of transaminases, γ-GT, ALP, total bilirubin, cholesterol, triglycerides associated with significant increase in the serum levels of T protein and albumin of 200 and 400 mg/kg bw of LSS protected rabbits for 5 and 10 weeks as compared with CCl4 treated rabbits. Oxidative stress and depressed antioxidant system of the liver tissues were markedly obvious in the CCl4 treated group. LSS administration reversed these results towards normalization. Histopathological examination of LSS protected rabbits (200 mg/kg bw of LSS for 10 weeks) showed improvement of the histoarchitectural changes of the liver induced by CCl4 to the normal aspect, showing regenerating hepatocytes with no steatosis, discrete chronic venous congestion, and discrete inflammatory infiltrate. The current findings provide new evidence that LSS could reverse the hepatotoxic effects of CCl4 and repair the liver functions.http://dx.doi.org/10.1155/2019/5947234 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mazin A. Zamzami Othman A. S. Baothman Fatma Samy Mohamed Kamel Abo-Golayel |
spellingShingle |
Mazin A. Zamzami Othman A. S. Baothman Fatma Samy Mohamed Kamel Abo-Golayel Amelioration of CCl4-Induced Hepatotoxicity in Rabbits by Lepidium sativum Seeds Evidence-Based Complementary and Alternative Medicine |
author_facet |
Mazin A. Zamzami Othman A. S. Baothman Fatma Samy Mohamed Kamel Abo-Golayel |
author_sort |
Mazin A. Zamzami |
title |
Amelioration of CCl4-Induced Hepatotoxicity in Rabbits by Lepidium sativum Seeds |
title_short |
Amelioration of CCl4-Induced Hepatotoxicity in Rabbits by Lepidium sativum Seeds |
title_full |
Amelioration of CCl4-Induced Hepatotoxicity in Rabbits by Lepidium sativum Seeds |
title_fullStr |
Amelioration of CCl4-Induced Hepatotoxicity in Rabbits by Lepidium sativum Seeds |
title_full_unstemmed |
Amelioration of CCl4-Induced Hepatotoxicity in Rabbits by Lepidium sativum Seeds |
title_sort |
amelioration of ccl4-induced hepatotoxicity in rabbits by lepidium sativum seeds |
publisher |
Hindawi Limited |
series |
Evidence-Based Complementary and Alternative Medicine |
issn |
1741-427X 1741-4288 |
publishDate |
2019-01-01 |
description |
The current study aimed to evaluate the probable protective effect of Lepidium sativum seeds (LSS) against CCl4 induced hepatic injury in New-Zealand rabbits. Rabbits were randomly divided into two main groups; group-A (noninjured group, n=15) was divided to subgroups A1 (untreated control) and A2 and A3 which received 200 & 400 mg/kg bw of LSS, respectively, in their diet daily. Group-B (injured group, n=30) were subcutaneously injected with CCl4 (0.5 ml/kg bw) starting from day one of the experiment and were equally divided into 3 subgroups: B1 received normal standard diet and B2 & B3 received 200 & 400 mg/kg bw of LSS, respectively, in their diet daily. Five rabbits of all subgroups were decapitated 5 and 10 weeks after experimental running. Biochemical analysis revealed significant decrease in serum levels of transaminases, γ-GT, ALP, total bilirubin, cholesterol, triglycerides associated with significant increase in the serum levels of T protein and albumin of 200 and 400 mg/kg bw of LSS protected rabbits for 5 and 10 weeks as compared with CCl4 treated rabbits. Oxidative stress and depressed antioxidant system of the liver tissues were markedly obvious in the CCl4 treated group. LSS administration reversed these results towards normalization. Histopathological examination of LSS protected rabbits (200 mg/kg bw of LSS for 10 weeks) showed improvement of the histoarchitectural changes of the liver induced by CCl4 to the normal aspect, showing regenerating hepatocytes with no steatosis, discrete chronic venous congestion, and discrete inflammatory infiltrate. The current findings provide new evidence that LSS could reverse the hepatotoxic effects of CCl4 and repair the liver functions. |
url |
http://dx.doi.org/10.1155/2019/5947234 |
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