Global rewiring of cellular metabolism renders Saccharomyces cerevisiae Crabtree negative

Global rewiring of cellular metabolism renders Saccharomyces cerevisiae Crabtree negative

Reducing aerobic ethanol production, a phenomenon referred to as the Crabtree effect, may open up new perspectives for using yeast as a cell factory. Here, the authors turn the Crabtree-positive yeast into Crabtree negative by combined engineering of cytosolic acetyl-CoA metabolism and mutating the...

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Main Authors: Zongjie Dai, Mingtao Huang, Yun Chen, Verena Siewers, Jens Nielsen
Format: Article
Language:English
Published: Nature Publishing Group 2018-08-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-018-05409-9
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spelling doaj-7016397e466f4d609b444d494707624d2021-05-11T09:51:40ZengNature Publishing GroupNature Communications2041-17232018-08-01911810.1038/s41467-018-05409-9Global rewiring of cellular metabolism renders Saccharomyces cerevisiae Crabtree negativeZongjie Dai0Mingtao Huang1Yun Chen2Verena Siewers3Jens Nielsen4Department of Biology and Biological Engineering, Chalmers University of TechnologyDepartment of Biology and Biological Engineering, Chalmers University of TechnologyDepartment of Biology and Biological Engineering, Chalmers University of TechnologyDepartment of Biology and Biological Engineering, Chalmers University of TechnologyDepartment of Biology and Biological Engineering, Chalmers University of TechnologyReducing aerobic ethanol production, a phenomenon referred to as the Crabtree effect, may open up new perspectives for using yeast as a cell factory. Here, the authors turn the Crabtree-positive yeast into Crabtree negative by combined engineering of cytosolic acetyl-CoA metabolism and mutating the RNA polymerase II mediator complex.https://doi.org/10.1038/s41467-018-05409-9
collection DOAJ
language English
format Article
sources DOAJ
author Zongjie Dai
Mingtao Huang
Yun Chen
Verena Siewers
Jens Nielsen
spellingShingle Zongjie Dai
Mingtao Huang
Yun Chen
Verena Siewers
Jens Nielsen
Global rewiring of cellular metabolism renders Saccharomyces cerevisiae Crabtree negative
Nature Communications
author_facet Zongjie Dai
Mingtao Huang
Yun Chen
Verena Siewers
Jens Nielsen
author_sort Zongjie Dai
title Global rewiring of cellular metabolism renders Saccharomyces cerevisiae Crabtree negative
title_short Global rewiring of cellular metabolism renders Saccharomyces cerevisiae Crabtree negative
title_full Global rewiring of cellular metabolism renders Saccharomyces cerevisiae Crabtree negative
title_fullStr Global rewiring of cellular metabolism renders Saccharomyces cerevisiae Crabtree negative
title_full_unstemmed Global rewiring of cellular metabolism renders Saccharomyces cerevisiae Crabtree negative
title_sort global rewiring of cellular metabolism renders saccharomyces cerevisiae crabtree negative
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2018-08-01
description Reducing aerobic ethanol production, a phenomenon referred to as the Crabtree effect, may open up new perspectives for using yeast as a cell factory. Here, the authors turn the Crabtree-positive yeast into Crabtree negative by combined engineering of cytosolic acetyl-CoA metabolism and mutating the RNA polymerase II mediator complex.
url https://doi.org/10.1038/s41467-018-05409-9
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AT mingtaohuang globalrewiringofcellularmetabolismrenderssaccharomycescerevisiaecrabtreenegative
AT yunchen globalrewiringofcellularmetabolismrenderssaccharomycescerevisiaecrabtreenegative
AT verenasiewers globalrewiringofcellularmetabolismrenderssaccharomycescerevisiaecrabtreenegative
AT jensnielsen globalrewiringofcellularmetabolismrenderssaccharomycescerevisiaecrabtreenegative
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