Incidence of multidrug-resistant pseudomonas spp. in ICU patients with special reference to ESBL, AMPC, MBL and biofilm production
Background: Multidrug-resistant (MDR) Pseudomonas spp. have been reported to be the important cause of ICU infections. The appearance of ESBL, AmpC and MBL genes and their spread among bacterial pathogens is a matter of great concern. Biofilm production also attributes to antimicrobial resistance du...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Wolters Kluwer Medknow Publications
2016-01-01
|
Series: | Journal of Global Infectious Diseases |
Subjects: | |
Online Access: | http://www.jgid.org/article.asp?issn=0974-777X;year=2016;volume=8;issue=1;spage=25;epage=31;aulast=Gupta |
id |
doaj-702773b4e786410e85ead8016ee26845 |
---|---|
record_format |
Article |
spelling |
doaj-702773b4e786410e85ead8016ee268452020-11-25T01:35:10ZengWolters Kluwer Medknow PublicationsJournal of Global Infectious Diseases0974-777X2016-01-0181253110.4103/0974-777X.176142Incidence of multidrug-resistant pseudomonas spp. in ICU patients with special reference to ESBL, AMPC, MBL and biofilm productionRicha GuptaAbida MalikMeher RizviS Moied AhmedBackground: Multidrug-resistant (MDR) Pseudomonas spp. have been reported to be the important cause of ICU infections. The appearance of ESBL, AmpC and MBL genes and their spread among bacterial pathogens is a matter of great concern. Biofilm production also attributes to antimicrobial resistance due to close cell to cell contact that permits bacteria to more effectively transfer plasmids to one another. This study aimed at determining the incidence of ESBL, AmpC, MBL and biofilm producing Pseudomonas spp. in ICU patients. Material and Methods: The clinical specimens were collected aseptically from 150 ICU patients from February 2012 to October 2013. Identification and antimicrobial susceptibility was performed according to Clinical and Laboratory Standards Institute (CLSI) guidelines. ESBLs and AmpC were detected phenotypically and genotypically. MBL was detected by modified Hodge and imipenem-EDTA double-disk synergy test. Results: Pseudomonas spp. 35(28%) were the most prevalent pathogen in ICU infections. Multidrug resistance and biofilm production was observed in 80.1% and 60.4% isolates, respectively. Prevalence of ESBL, AmpC and MBL was 22.9%, 42.8% and 14.4%, respectively. The average hospital stay was 25 days and was associated with 20% mortality. Conclusions: A regular surveillance is required to detect ESBL, AmpC and MBL producers especially in ICU patients. Carbapenems should be judiciously used to prevent their spread. The effective antibiotics, such as fluoroquinolones and piperacillin-tazobactum should be used after sensitivity testing.http://www.jgid.org/article.asp?issn=0974-777X;year=2016;volume=8;issue=1;spage=25;epage=31;aulast=GuptaICUmultidrug resistancepseudomonas |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Richa Gupta Abida Malik Meher Rizvi S Moied Ahmed |
spellingShingle |
Richa Gupta Abida Malik Meher Rizvi S Moied Ahmed Incidence of multidrug-resistant pseudomonas spp. in ICU patients with special reference to ESBL, AMPC, MBL and biofilm production Journal of Global Infectious Diseases ICU multidrug resistance pseudomonas |
author_facet |
Richa Gupta Abida Malik Meher Rizvi S Moied Ahmed |
author_sort |
Richa Gupta |
title |
Incidence of multidrug-resistant pseudomonas spp. in ICU patients with special reference to ESBL, AMPC, MBL and biofilm production |
title_short |
Incidence of multidrug-resistant pseudomonas spp. in ICU patients with special reference to ESBL, AMPC, MBL and biofilm production |
title_full |
Incidence of multidrug-resistant pseudomonas spp. in ICU patients with special reference to ESBL, AMPC, MBL and biofilm production |
title_fullStr |
Incidence of multidrug-resistant pseudomonas spp. in ICU patients with special reference to ESBL, AMPC, MBL and biofilm production |
title_full_unstemmed |
Incidence of multidrug-resistant pseudomonas spp. in ICU patients with special reference to ESBL, AMPC, MBL and biofilm production |
title_sort |
incidence of multidrug-resistant pseudomonas spp. in icu patients with special reference to esbl, ampc, mbl and biofilm production |
publisher |
Wolters Kluwer Medknow Publications |
series |
Journal of Global Infectious Diseases |
issn |
0974-777X |
publishDate |
2016-01-01 |
description |
Background: Multidrug-resistant (MDR) Pseudomonas spp. have been reported to be the important cause of ICU infections. The appearance of ESBL, AmpC and MBL genes and their spread among bacterial pathogens is a matter of great concern. Biofilm production also attributes to antimicrobial resistance due to close cell to cell contact that permits bacteria to more effectively transfer plasmids to one another. This study aimed at determining the incidence of ESBL, AmpC, MBL and biofilm producing Pseudomonas spp. in ICU patients. Material and Methods: The clinical specimens were collected aseptically from 150 ICU patients from February 2012 to October 2013. Identification and antimicrobial susceptibility was performed according to Clinical and Laboratory Standards Institute (CLSI) guidelines. ESBLs and AmpC were detected phenotypically and genotypically. MBL was detected by modified Hodge and imipenem-EDTA double-disk synergy test. Results: Pseudomonas spp. 35(28%) were the most prevalent pathogen in ICU infections. Multidrug resistance and biofilm production was observed in 80.1% and 60.4% isolates, respectively. Prevalence of ESBL, AmpC and MBL was 22.9%, 42.8% and 14.4%, respectively. The average hospital stay was 25 days and was associated with 20% mortality. Conclusions: A regular surveillance is required to detect ESBL, AmpC and MBL producers especially in ICU patients. Carbapenems should be judiciously used to prevent their spread. The effective antibiotics, such as fluoroquinolones and piperacillin-tazobactum should be used after sensitivity testing. |
topic |
ICU multidrug resistance pseudomonas |
url |
http://www.jgid.org/article.asp?issn=0974-777X;year=2016;volume=8;issue=1;spage=25;epage=31;aulast=Gupta |
work_keys_str_mv |
AT richagupta incidenceofmultidrugresistantpseudomonassppinicupatientswithspecialreferencetoesblampcmblandbiofilmproduction AT abidamalik incidenceofmultidrugresistantpseudomonassppinicupatientswithspecialreferencetoesblampcmblandbiofilmproduction AT meherrizvi incidenceofmultidrugresistantpseudomonassppinicupatientswithspecialreferencetoesblampcmblandbiofilmproduction AT smoiedahmed incidenceofmultidrugresistantpseudomonassppinicupatientswithspecialreferencetoesblampcmblandbiofilmproduction |
_version_ |
1725068088970313728 |